Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal Universa Medicina JKKI : Jurnal Kedokteran dan Kesehatan Indonesia
Kuswati Kuswati
Department of Anatomy, Faculty of Medicine, Islamic University of Indonesia
Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Centella asiatica increases B-cell lymphoma 2 expression in rat prefrontal cortex Kuswati Kuswati; Djoko Prakosa; Brian Wasita; Nanang Wiyono
Universa Medicina Vol. 34 No. 1 (2015)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2015.v34.10-16

Abstract

BACKGROUNDStress is one of the factors that cause apoptosis in neuronal cells. Centellaasiatica has a neuroprotective effect that can inhibit apoptosis. This studyaimed to examine the effect of Centella asiatica ethanol extract on B-celllymphoma 2 (Bcl-2) protein expression in the prefrontal cortex of rats.METHODSAn experimental study was conducted on 34 brain tissue samples from maleSprague Dawley rats exposed to chronic restraint stress for 21 days. Thesamples were taken from following groups: non-stress group K, negativecontrol group P1 (stress + arabic gum powder), P2 (stress + C.asiatica at150 mg/kgBW), P3 (stress + C.asiatica at 300 mg/kg BW), P4 (stress +C.asiatica at 600 mg/kg body weight) and positive control group P5 (stress+ fluoxetine at 10 mg/kgBW). The samples were made into sections thatwere stained immunohistochemically using Bcl-2 antibody to determine thepercentage of cells expressing Bcl-2. Data were analyzed using one wayANOVA test followed by a post - hoc test.RESULTSThere were significant differences in mean Bcl-2 expression between thegroups receiving Centella asiatica compared with the non-stress group andstress-only group (negative control group) (p<0.05). The results werecomparable to those of the fluoxetine treatment group.CONCLUSIONThe Centella asiatica ethanol extract was able to increase Bcl-2 expressionin the prefrontal cortex of Sprague Dawley rats exposed to restraint stress.This study suggests that Centella asiatica may be useful in the treatment ofcerebral stress.
The effect of centella asiatica ethanolic extract on caspase-3 expression in prefrontal cortex of chronic restraint stress induced sprague dawley rat Kuswati Kuswati
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 7, No 2, (2015)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol7.Iss2.art6

Abstract

Introduction: Stress cause changes in the structure and function of the prefrontal cortex. Chronic restraint stress causes the shortening of dendrites in the medial prefrontal cortex. Centella asiatica is an herb that has antioxidant and neuroprotective effects. The purpose of this study is to understand the effect of Centella asiatica on the active protein caspase-3 apoptosis executor expression in the prefrontal cortex. Methods: This study was a laboratory experimental using posttest only control group design. The study used 25 pieces of stored biological material sample in the form of rat brain tissue paraffin blocks. These brain tissues came from 25 rats that were divided into groups of P1, P2, P3, P4 and P5. P1 group treated with restraint stress. Group P2, P3 and P4 were subjected to restraint stress and Centella asiatica ethanolic extract with therapeutic dose of 150, 300 and 600 mg/kg/day. P5 group received stress treatment and fluoxetine with therapeutic dose of 10 mg/kg/day. On the 23rd day, termination, perfusion and brain tissues sampling were executed. Furthermore, the brain tissues were made into paraffin blocks, cut into specimen, immunohistochemical stained, and count the cells which were expressing active caspase-3. Results: There were significant differences in the expression of active caspase-3 between P1 to P2, P3, P4 and P5 groups (p = 0.00). There were no significant differences between P2, P3, P4 and P5 groups. Conclusion: Centella asiatica ethanolic extract can inhibit active caspase-3 expression in the prefrontal cortex of Sprague Dawley rats that were induced with chronic restraint stress.
Co-Authors Brian Wasita Djoko Prakosa Nanang Wiyono