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All Journal Biogenesis: Jurnal Ilmiah Biologi Jurnal Kesehatan Poltekkes Kemenkes RI Pangkalpinang
Auronita Puspa Pratiwi
Poltekkes Kemenkes Pangkalpinang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Variasi Genetik Attacus atlas L. (Lepidoptera: Saturniidae) Berdasar Penanda Molekuler ISSR (Inter Simple Sequence Repeat) Auronita Puspa Pratiwi
Biogenesis: Jurnal Ilmiah Biologi Vol 2 No 1 (2014)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v2i1.464

Abstract

Attacus atlas is one of potent silk-producing insect and possesses a significant economic value. A. atlas is part of genus Attacus which inhabits in several quite large areas. A.atlas can be found in Sumatera, Java, Borneo, Halmahera Islands, and Papua. Mostly, species which inhabits in wide spread area, has a high level of diversity. Applied studies on Antheraea dan Samia cynthia ricini showed that geographical differences led to genetic variations occurred upon those species. The aim of this research was to study and analyze the genetic variation A. Atlas, which is obtained from multiple sampling locations, ie Berbah, Dlingo, Ngawen, and Pracimantoro, based on ISSR marker. Analysis of genetic variation performed with 4 ISSR primer, ISSR1, ISSR2, ISSR6, and ISSR7 gave forth high genetic variations among individuals of A.atlas by indicating the average polymorphic percentage of all primer of 97,78%. Clustering analysis with unweighted pair group with arithmetic average (UPGMA) method resulted dendrogram that exhibiting similarity values among individuals in range between 61% - 87% and clustered into two main clusters at 61% similarity value. The first cluster consists of samples taken from from Dlingo, Ngawen and Pracimantoro which has elevation above 200 m asl, while the second cluster consists of samples originating from Berbah which has elevation below 200 m asl. Amplification used 4 ISSR primer performed 2 monomorphic loci and 82 polymorphic loci. Monomorphic at 300 bp showed by ISSR2 and at 1800 bp showed by ISSR7 may be a candidate locus identifier species of A. atlas.Keywords: Attacus atlas, genetic variation, PCR-ISSR
POTENSI ANTIBAKTERI EKSTRAK DAUN PELAWAN MERAH (Tristaniopsis merguensis Griff.) Auronita Puspa Pratiwi
JURNAL KESEHATAN POLTEKKES KEMENKES RI PANGKALPINANG Vol 7, No 1 (2019): JKP Juni 2019
Publisher : Poltekkes Kemenkes Pangkalpinang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32922/jkp.v7i1.79

Abstract

Daun Pelawan Merah dikenal masyarakat sebagai tumbuhan yang dimanfaatkan untuk obat tradisional diare. Diduga kandungan fitokimianya berpotensi sebagai bahan alami antibiotik. Penelitian ini bertujuan untuk melihat potensi daun Pelawan Merah sebagai antibakteri. Ekstrak dibuat dengan 3 pelarut, yaitu etanol, etil asetat dan n-heksana. Pengujian dilakukan dengan metode difusi cakram terhadap 3 bakteri yaitu Staphylococcus aureus, Escherichia coli, dan Bacillus subtilis. Aktivitas antibakteri diketahui melalui pengukuran diameter zona hambat yang terbentuk pada media bakteri setelah diberi perlakuan dan diinkubasi selama 24 jam. Hasil penelitian menunjukkan ekstrak n-Heksana memiliki daya hambat lemah terhadap S.aureus dan B.subtilis. Ekstrak etanol menunjukkan daya hambat lemah hingga sedang terhadap S.aureus, sedangkan pada E.coli dan B.subtilis tampak adanya daya hambat sedang. Ekstrak etil asetat menunjukkan daya hambat sedang terhadap E.coli, dan daya hambat lemah hingga kuat terhadap B.subtilis dan S.aureus.
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