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All Journal Biogenesis: Jurnal Ilmiah Biologi Biodiversitas Journal of Biological Diversity
Mohd Yunus Shukor
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Arrhenius Plot Analysis of the Temperature Effect on the Biodegradation Rate of 2-chloro-4-nitrophenol Mohd Yunus Shukor
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.17419

Abstract

Several models are available to determine the effect of temperature on the growth rate of microorganisms on substrates. An example is Arrhenius model, which is very popular because it has few parameters. For the first time, a discontinuous chevron-like graph of apparent activation energy based on the Arrhenius plot on the growth of 2-chloro-4-nitrophenol by Cupriavidus sp. is reported. The plot of ln mm against 1/T shows a discontinuous chevron-like graph for the entire investigated temperature range with an inflection at 27.75°C. This indicates that the existence of 2 activation energies for growth on 2-chloro-4-nitrophenol ranges from 20 to 40°C. Furthermore, a regression analysis from 20–25°C and 30–40°C results in activation energies of 88.71 kJmol-1 and 75.16kJ mol-1, respectively. This is probably the first time a Chevron-like graph was observed for the Arrhenius plot on the effect of temperature on the growth rate of 2-chloro-4-nitrophenol.
Isolation and characterization of a molybdenum-reducing and SDSdegrading Klebsiella oxytoca strain Aft-7 and its bioremediation application in the environment NORAZLINA MASDOR; MOHD SHUKRI ABD SHUKOR; AFTAB KHAN; MOHD IZUAN EFFENDI BINMOHD HALMI; SITI ROZAIMAH SHEIKH ABDULLAH; NOR ARIPIN SHAMAAN; MOHD YUNUS SHUKOR
Biodiversitas Journal of Biological Diversity Vol. 16 No. 2 (2015)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d160219

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Masdor N, Shukor MSA, Khan A, Halmi MIE, Abdullah SRS, Shamaan NA, Shukor MY. 2015. Taxonomy and distribution of species of the genus Acanthus (Acanthaceae) in mangroves of the Andaman and Nicobar Islands, India. Biodiversitas 16: 238-246.Pollution as a result of anthropogenic activities is a severe global issue. These activities including inappropriate disposal, industrialand prospecting activities and unnecessary use of agricultural chemicals have triggered international initiatives to eliminate thesecontaminants. In this work we screen the ability of a molybdenum-reducing bacterium isolated from contaminated soil to grow andreduce molybdenum on various detergents. The bacterium was able to grow on SDS as a carbon source although the compound did notsupport molybdenum reduction. The bacterium reduces molybdate to Mo-blue optimally between pH 5.8 and 6.3 and between 25 and34oC. Glucose was the best electron donor for supporting molybdate reduction followed by sucrose, D-mannitol, D-sorbitol, lactose,salicin, trehalose, maltose and myo-Inositol in descending order. Other requirements include a phosphate concentration between 5.0 and7.5 mM and a molybdate concentration between 5 and 20 mM. The absorption spectrum of the Mo-blue produced was similar toprevious Mo-reducing bacterium, and closely resembles a reduced phosphomolybdate. Molybdenum reduction was inhibited by mercury(ii), silver (i) and copper (ii) at 2 ppm by 62.1, 33.9 and 33.6%, respectively. Biochemical analysis resulted in a tentative identificationof the bacterium as Klebsiella oxytoca strain Aft-7. The ability of this bacterium to detoxify molybdenum and degrade detergent makesthis bacterium an important tool for bioremediation.
Co-Authors AFTAB KHAN MOHD IZUAN EFFENDI BINMOHD HALMI MOHD SHUKRI ABD SHUKOR NOR ARIPIN SHAMAAN NORAZLINA MASDOR SITI ROZAIMAH SHEIKH ABDULLAH