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Ahmad Syauqi
Department of Biology, Faculty of Mathematics and Natural Sciences, Islamic University of Malang (UNISMA)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Materials Science & Nanotechnology

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Comparative Study of References and Protein Quantifications Using Biuret-Spectrophotometric Method Ahmad Syauqi; Muhamad Fuadi; Hari Santoso
Chimica et Natura Acta Vol 6, No 2 (2018)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.216 KB) | DOI: 10.24198/cna.v6.n2.19224

Abstract

Comparison between the references and the quantification of biuret standard curves can be performed using statistical methods. The objective of this study was to select a method of protein quantification of the biuret-spectrophotometric based on the similar standard curve of the compound. The study used experimental methods in the laboratory and references from a comparative scheme of mean that has categories of statistics called academic and practical terms. Two standard curves were tested with the reference data. The academic way was performed with a comparison of 11.779-12.401% confidence, and the quantification of the results of the protein mean of 8.211% and 10.17% showed no significant difference. The practical methods were carried out with the original reference data of 10.8-12.8%, and the result of the test confidence values of 9.163-11.180% and 7.596-8.826% showed different accuracy results. The quantifications of the protein biuret method displayed different results on how to compare according to academic and practical ways. The quantification method using the Biuret-spectrophotometric practical way shows accuracy by a certain standard curve compared with the original values of references.
Co-Authors Hari Santoso Muhamad Fuadi