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Tek An Lie
Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University
Agriculture, Biological Sciences & Forestry

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EFFECT OF PHOSPHATE ON NODULE PRIMORDIA OF SOYBEAN (Glycine max Merrill) IN ACID SOILS IN RHIZOTRON EXPERIMENTS Setiyo Hadi Waluyo; Tek An Lie; Leendert ’t Mannetje
Indonesian Journal of Agricultural Science Vol 5, No 2 (2004): October 2004
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v5n2.2004.p37-44

Abstract

To clarify whether P had a direct or indirect effect on the nodulation  process of soybean grown in acid soils from Sitiung, West Sumatra, Indonesia, a series of rhizotron experiments, with special attention given to formation of nodule primordia, was conducted at Laboratory of  Microbiology, Wageningen University in 1998-2000. It was shown that Ca and P were essential nutrients for root growth, nodule formation, and growth of soybean in the acid soils (Oxisols). Ca increased root growth, number of nodule primordia, nodules, and growth of the soybean plant. This positive effect of Ca was increased considerably by the application of P. Ca and P have a synergistic effect on biological nitrogen fixation (BNF) of soybean in acid soils. Ca is important for the establishment of nodules, whilst P is essential for the development and function of the formed nodules. P increased number of nodule primordia, thus it also has an important role in the initiation of nodule formation. From this study, it can be concluded that Ca and P are the most limiting nutrients for BNF of soybean in the acid soils of Sitiung, West Sumatra, Indonesia.
CHARACTERISATION OF SOYBEAN RHIZOBIAL STRAINS FROM JAVA AND SUMATRA Setiyo Hadi Waluyo; Tek An Lie; Leendert’t Mannetje; Willem M. de Vos
Indonesian Journal of Agricultural Science Vol 6, No 1 (2005): April 2005
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v6n1.2005.p10-19

Abstract

To get insight in the structure of soybean rhizobial population native to Indonesian soils, a thorough survey of the occurrence of the soybean rhizobia were conducted in several locations in Java and Sumatra. A total of 51 different isolates of rhizobial strains were characterised phenotypically based on their symbiotic properties, and genetically using amplified ribosomal DNA restriction analysis (ARDRA). Based on their nodulation capacity on both soybean and the native legume mungbean, these rhizobial strains could be divided into a group of 16 strains specific for soybean only and another group of 35 promiscuous strains that nodulated both leguminous plants. Based on ARDRA of PCRamplified 16S rDNA and 16S-23S rDNA spacer fragments, the rhizobial strains isolated from Java differed with those from Sumatra. Six Java isolates and only one Sumatra isolate were classified as Bradyrhizobium japonicum and these similar to that of B. japonicum strain USDA 110. All these B. japonicum strains were highly specific for soybean. One isolate from Java showed a rather unique position. The remaining strains from Java (20), which were symbiotically promiscuous strains, were clustered in another group. This group and another group containing most Sumatra isolates were distinct from B. japonicum USDA 110 and therefore it is tempting to speculate that these represent indigenous soybean rhizobial bacteria. Application of agricultural practices, such as enhancement of rhizobial population, to increase soybean production is still essential and noteworthy in Sumatra.
Co-Authors Leendert ’t Mannetje Leendert’t Mannetje Setiyo Hadi Waluyo Willem M. de Vos