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BIOLOGICAL CONTROL OF BANDED LEAF AND SHEATH BLIGHT DISEASE (Rhizoctonia solani KUHN) IN CORN WITH FORMULATED Bacillus subtilis BR23 Amran Muis; Arcadio J. Quimio
Indonesian Journal of Agricultural Science Vol 7, No 1 (2006): April 2006
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v7n1.2006.p1-7

Abstract

Rhizoctonia solani Kuhn. causing banded leaf and sheath blight diseases is one of the important fungi of corn world wide. The fungus is commonly controlled by using fungicide because no resistant variety available. The objective of the study was to develop a seed treatment formulation of the selected Bacillus subtilis to control R. solani in corn. The study was conducted in the Department of Plant Pathology, College of Agriculture, University of the Philippines Los Bañòs, College, Laguna from May 2004 to August 2005, using sweet corn var. IPB Supersweet as test plant. Corn seeds were surface sterilized for 10 minutes in 1% sodium hypochlorite solution and 5% ethanol, washed thrice with sterile distilled water and air-dried. The seeds were coated with formulated B. subtilis BR23 and used for several experiments, such as evaluation for their germination and growth in the laboratory, effectively on R. solani in the baked and nonbaked field soil under greenhouse condition, and in the microplots artificially infested with R. solani. The treatment was compared with other standard seed treatment of synthetic fungicides such as captan (10 g per kg seeds) and metalaxyl (10 g per kg seeds). The experiments were designed in a completely random design with three replications. Parameters observed were seed germination, plant height, disease scores, and plant yield. Laboratory formulated B. subtilis BR23 used as seed treatment had no detrimental effects on seed germination and seedling vigor. In microplots artificially infested with a selected highly virulent R. solani, seed treatment with the same formulation increased grain yield by 27% compared to that of the control captan seed treatment with 14.4%. The studies showed the potential of B. subtilis BR23 for commercialization as a seed treatment for the control of banded leaf and sheath blight disease (R. solani) in corn.
The Response of Foxtail Millet Candidate Varieties from Nagekeo Regency to Leaf Blight (Bipolaris setariae) Suriani Suriani; Amran Muis; Septian Hary Kalqutny
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 5 No 1 (2021): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (920.153 KB) | DOI: 10.33019/agrosainstek.v5i1.207

Abstract

Foxtail millet has the potential to be developed as a healthier food alternative because of its high nutritional value. Disease such as leaf blight caused by Bipolaris setariae is one of the limiting factors in Foxtail millet productivity. One of the efforts to control the pathogen is by utilizing resistant varieties. In this study, two candidate varieties and two germplasm accessions were tested to determine the level of resistance to Bipolaris setariae leaf blight. The study was arranged based on a complete randomized design with six replications. Each test material was inoculated with the spore suspension at 4 WAP. Disease intensity was observed based on the disease scoring at 7, 9, and 11 WAP. AUDPC value is calculated based on the intensity of the attack at a particular observation time. Grain weight was recorded and statistically analyzed. The two candidate varieties of foxtail millet Pagamogo and Tedamude from Nagekeo Regency showed a moderately resistant response to leaf blight and had the lowest AUDPC values of 907.69 and 912.31. The highest increase in AUDPC values was observed in the initial observation period at 0-49 DAP.