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All Journal Indonesian Journal of Agricultural Science Jurnal AgroBiogen
Wage Ratna Rohaeni
Indonesian Center for Rice Research Jl. Raya 9 Sukamandi Subang Jawa Barat
Agriculture, Biological Sciences & Forestry

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LOW ASSOCIATION OF Bph17 ALLELE IN LANDRACES AND IMPROVED VARIETIES OF RICE RESISTANT TO BROWN PLANTHOPPER Wage Ratna Rohaeni; Untung Susanto; Aida F.V. Yuningsih
Indonesian Journal of Agricultural Science Vol 18, No 1 (2017): June 2017
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v18n1.2017.p1-6

Abstract

Resistance traits to brown planthopper on rice varieties are controlled by dominant and recessive genes called Bph/bph. Bph17 is one of dominant genes that control rice resistance to brown planthopper.  Marker of Bph17 allele can be used as a tool of marker assisted selection (MAS) in breeding activity. Association of Bph17 allele and resistance to brown planthopper in Indonesian landraces and new-improved varieties of rice is not clearly known. The study aimed to determine the association of Bph17 allele in landraces and new-improved varieties of rice resistant to brown planthopper. Twenty-one rice genotypes were used in the study, consisting of 13 landraces, 5 improved varieties, 3 popular varieties and a check variety Rathu Heenati. Two simple sequence repeat markers linked to Bph17 allele were used, i.e. RM8213 and RM5953. The results showed that association of Bph17 allele in landraces and new-improved varieties of rice resistant to brown planthopper resistance was very low (r = -0.019 and -0.023, respectively). The presence of Bph17 allele did not constantly express resistance to brown planthopper. The study suggests that Bph17 allele cannot be used as a tool of MAS for evaluating resistance of landraces and new-improved varieties of rice to brown planthopper. Further research is needed to obtain a specific gene marker that can be used as a tool of MAS and applicable for Indonesian differential rice varieties. 
Genotypic Analysis of Two Local Swamp Rice Based on Microsatellite Markers Wage Ratna Rohaeni; Indrastuti A. Rumanti
Jurnal AgroBiogen Vol 17, No 2 (2021): DESEMBER
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v17n2.2021.p75-82

Abstract

Argo is a local swamp rice from Kalimantan which is widely cultivated by local farmers. Exploration activities in Kalimantan found two types of local rice named Argo but demonstrated different phenotypes. Therefore, genotypic verification is needed to determine the differences between the two Argo rice genotypes. DNA fingerprint could help genotype verification rapidly and the result is not influenced by environmental factors. The aims of this study were to determine the level of similarity of Argo-1 and Argo-2 with swamp rice and other irrigated rice genotypes using microsatellite markers. A total of eight improved and local varieties were used as genetic material with the two local rice Argo (Argo-1 and Argo-2). The rice genotypes were analyzed by using eight microsatellite markers. The results of the molecular analysis showed that the two types of Argo showed different DNA bands based on the RM228 marker but genetically they were closely related. The genetic distance between the two Argo cultivars is 0.143. Argo-1 and Argo-2 were located in the same cluster as Inpara 4 and Siam KDK, but they were in the different groups from those of irrigated rice varieties such as Mekongga and Inpari 9. Based on this research, Argo-1 and Argo-2 are not recommended to be crossed with Inpara 4 and Siam KDK because the genetic diversity formed will be narrow.
Co-Authors Aida F.V. Yuningsih Indrastuti A. Rumanti Untung Susanto