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Sriwuryandari, L. -
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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OUTDOORS BATCH CULTIVATION OF MARINE MICROALGAE Nannochloropsis sp USING PARALLEL GLASS TUBULAR PHOTOBIOREACTOR Astuti, J.T -; Sriwuryandari, L. -; Priantoro, E.A -; Sembiring, T -
Bionatura Vol 14, No 3 (2012): Bionatura Nopember 2012
Publisher : Direktorat Sumber Daya Akademik dan Perpustakaan

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ABSTRACTMicroalgae Nannochloropsis sp is autotrophic organism that can utilize atmospheric CO2 and sunlightfor growth via photosynthesis. It is potential sources for biodiesel due to its high lipid content. Formass production, two basic designs of photo-bioreactor are available, i.e. open and enclosed systems.Study was conducted to investigate the growth performance of Nannochloropsis sp in outdoor batchculture system using Parallel Enclosed Glass-Tubular Photo-bioreactor (PBR) for 18 days withmodified f/2 medium. Nutrient N-P was calculated based on the empiric structure of microalgae cell(C1O0.48H1.83N0.11P0.01) with target of biomass yield was 1gL-1. Temperature, pH, Optical Density (OD),Dry Cell Weight (DCW), population, lipid content, and fatty acid were observed. Data showed thattemperature fluctuated between 22oC and 35oC, pH was stable at 7.0-7.5, OD increased from 0.134 to0.878, DCW increased 39 times higher than its initial value. At the 18th day, the cell diameter variedin range of 1-9 μm, cell weight increased 5.3 times (3.5 to 18.4 pg.cell-1), and lipid content is 35.71%.Fatty acid was composed by saturated fatty acids (92.39%) that covered of lauric (20.30%), myristic(12.69%), palmitic (50.05%), and stearic (9.35%) that was suitable for biodiesel. Unsaturated fatty acidwas performed by oleic acid (3.49%).Key words: CO2 mitigation, biodiesel, Nannochloropsis sp, photo bioreactor
BIODIESEL DARI MIKROALGA: PERBANYAKAN BIOMASSA MELALUI PENAMBAHAN NUTRISI SECARA BERTAHAP Astuti, J T -; Sriwuryandari, L. -
Bionatura Vol 12, No 3 (2010): Bionatura Nopember 2010
Publisher : Direktorat Sumber Daya Akademik dan Perpustakaan

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Abstract

Pemanfaatan sinar matahari dan CO2 pada fotosintesis oleh mikroalga adalah satu teknologi untuk memperoleh energi alternatif yang dapat diperbaharui. Energi tersebut tersimpan dalam biomassa sel dan dapat diubah menjadi biodiesel. Pertumbuhan dan komposisi sel mikroalga dipengaruhi oleh straindan kondisi lingkungan, termasuk suhu dan nutrisi. Tujuan penelitian adalah untuk optimasi produksibiomasa Nannochloropsis sp melalui penambahan nutrisi secara bertahap. Penelitian menggunakan erlenmeyer berisi 400 ml media f/2. NaHCO3 digunakan sebagai sumber C, sedangkan NH4NO3 dan KH2PO4 sebagai sumber N dan P ditambahkan bervariasi, yaitu sesuai rumus empiris sel mikroalga (N0P0); N berlebih 10% (N10P0); P berlebih 10% (N0P10); dan N-P berlebih 10% (N10P10), Penambahan dalam tiga tahap. Biomasa dipanen pada hari ke 35 dan pengamatan meliputi suhu, pH, optical density (OD), berat kering sel (BKS), kadar khlorofil-a dan lipid. Suhu media berkisar 24,0-33.5oC dan pH stabil antara 9.0-9.5. OD meningkat tajam dari 0,213-0.266 ke 1,74-2.31. Kadar klorofil-a berkisar 78,44-95,11 mg.L-1. Produksi biomasa kering cukup tinggi, yaitu antara 1,61-2,73 g.L-1 dan kadar lipid antara 15,26-34,37% BKS. Nilai terbaik diperoleh dari perlakuan N10P0 dengan produksi biomasa 2,73 g.L-1 dan kadar lipid 34,37% BKS. Dapat disimpulkan penambahan nutrisi C, N, P secara bertahap dapat meningkatkan produksi biomasa. Asam lemak Nannochloropsis didominasi oleh asam palmitat, laurat, dan miristat.Kata kunci: biodiesel, lipid, mikroalga, Nannochloropsis sp, nutrisi.
Co-Authors Astuti, J T - Astuti, J.T - Priantoro, E.A - Sembiring, T -