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Ika Mariska
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
Agriculture, Biological Sciences & Forestry

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Perbanyakan Tanaman Jambu Mete (Anacardium occidentale L.) melalui Jalur Organogenesis Rossa Yunita; Ika Mariska; Christiani Tumilisar
Jurnal AgroBiogen Vol 8, No 3 (2012): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n3.2012.p113-119

Abstract

Vegetativepropagation through in vitro culture has been carried out asa technology that has the potential for obtaining seedling insignificant amounts and relatively faster. This activity can bedone through the multiplication of adventitious shoots andlateral shoots (organogenesis). The goal of this research wasto find the method of cashew micropropagation throughorganogenesis. This study consisted of 4 main activities.They were shoot induction, shoot multiplication, shootelongation, and root induction. The results showed the bestmedium composition for shoot induction was MS + BA 0.7mg/l. The suitable media for shoots multliplication was MS +thidiazuron 0.5 mg/l + zeatin 1 mg/l and for shootselongation was MS + GA 1 mg/l + zeatin + 3 mg/l. The bestmethods for root induction was by submerging in vitroshoots in a solution of IAA 100 mg/l.
Analisis Gen Selubung Protein Chilli Veinal Mottle Potyvirus dari Beberapa Daerah di Indonesia Ifa Manzila; Sri H. Hidayat; Ika Mariska; Sriani Sujiprihati
Jurnal AgroBiogen Vol 8, No 1 (2012): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n1.2012.p27-37

Abstract

Variation on symptoms and virulence wasobserved on different isolates of ChiVMV collected fromWest Java, Central Java, East Java, South Kalimantan, WestSumatera and Central Aceh. Research was conducted tostudy genetic variation of six ChiVMV isolates based onsequence analysis of coat protein (CP) gene and aminoacid. Sequence analysis of CP gene showed 87% to 99%homology among the six isolates with level of variationranging from 0.02% to 1.48%. Sequence analysis of aminoacid derived from CP gene showed 85% to 99% homology.Further analysis on amino acid motives of CP gene indicatedmutation of octapeptide motif, i.eLSGQVQPQSRQSEMETEVPQVR on ChiVMV CKB andRMETFGLDGRVGTQEEDTERHT on other ChiVMV isolates.Other differences was observed on amino acid number 61and 84 i.e. deletion of MET and mutation of GG to KV onChiVMV BL and KR. Phyllogenetic analysis based onnucleotide and amino acid sequence showed that sixisolates of ChiVMV can be differentiated into three groups.ChiVMV KR and BL were in the same group with ChiVMVPataruman (GeneBank No. access DQ854961), ChiVMV CKBwas in the same group with ChiVMV Cikabayan 2(GeneBank No. access DQ854960), and ChiVMV TD, ChiVMVNI and GB ChiVMV were in the same group with ChiVMVTaiwan (GeneBank No. access DQ854948). Analysis of CPgene confirmed the occurrence of genetic variation amongChiVMV isolates although symptom variation is weak.
Regenerasi Jeruk Siam melalui Embriogenesis Somatik Ali Husni; Agus Purwito; Ika Mariska; Sudarsono Sudarsono
Jurnal AgroBiogen Vol 6, No 2 (2010): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v6n2.2010.p75-83

Abstract

The Regenerated of Siam Tangerin through SomaticEmbryogenesis. Ali Husni, Agus Purwito, Ika Mariska,and Sudarsono. Somatic embryogenesis occurs in mostplants that are cultured on a suitable medium in vitro.Somatic embryo may arise from single cells and the embryogeniccells are widely applicable in plant propagation,genetic manipulation and transgenic technologies. Thepresent study was carried out to develop an effectivesomatic embryogenesis technique to regenerate Siamtangerine plants. Materials used in this study were nucellartissues of young fruits (30-90 days post anthesis). Inductionof embryogenic calli was done by culturing the tissues onthree different basal media (MS, MW and MT). Embryomaturation was done on the MW medium + ABA (0; 0.1; 0.3;dan 0.5 mg/l), while germination to plantlet developmentwas done on WS medium + GA3 (0, 0.1, 0.3, and 0.5 mg/l).The results showed that among the three media, MW wasthe best medium for callus induction from nucellar tissueswith a success rates 98% for Simadu and 100% forPontianak. The best maturation of embryo somatic wasdone on MW medium + ABA 0.5 mg/l with success rates99%, while the best medium for germination and developmentinto planlets was MW medium + 0.5 mg/l GA3 with asuccess rate 58%.
Co-Authors Agus Purwito Ali Husni Christiani Tumilisar Ifa Manzila Rossa Yunita Sri H. Hidayat Sriani Sujiprihati Sudarsono