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Sri Hutami
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111
Agriculture, Biological Sciences & Forestry

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TINJAUAN Penggunaan Suspensi Sel dalam Kultur In Vitro Sri Hutami
Jurnal AgroBiogen Vol 5, No 2 (2009): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v5n2.2009.p84-92

Abstract

Cell suspension culture could be defined as aprocess that allows rapidly dividing homogenous suspensionof cells to grow in liquid nutrient media. There are two maintypes of suspension cultures: (1) Batch cultures in whichcells are nurtured in a fixed volume of medium until growthceases and (2) Continuous cultures in which cell growth ismaintained by continuous replenishment of sterile nutrientmedia. Plant cell suspension cultures are mostly used for thebiochemical investigation of cell physiology, growth, metabolism,protoplast fusion, transformation and for large scaleproduction of seed by bioreactor and production of secondarymetabolites. Contamination is one of the largest problemswhen dealing with cell cultures. Differences betweenthe products of cell suspension culture and whole plant arefrequently observed. These phenomena’s may be resultedfrom lack of differentiation and organization and cell cultureinducedvariation. Utilization of cell suspension culture inIndonesia is still limited, some of them for mass productionof plantation seed with bioreactor system and for productionof secondary metabolites. The success of this study give theopportunity for mass production of seeds from other plantsand also production of secondary metabolites.
ULASAN Masalah Pencoklatan pada Kultur Jaringan Sri Hutami
Jurnal AgroBiogen Vol 4, No 2 (2008): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v4n2.2008.p83-88

Abstract

Severaltropical plant species contain high concentrations of phenoliccompounds, which become oxidised when their cellsare wounded or when the plant parts become senescences.In tissue culture, the phenolic compounds usually leach intothe medium from the cut surfaces of explants. The phenoliccompounds caused the culture medium turns to dark brownin colour due to oxidation. This is detrimental to the culture,because it causes the isolated tissue fails to grow. Thebrowning of tissue culture and the medium can often beprevented by one of the several different approaches, suchas by removing the phenolic compounds produced, modifyingthe redox potential, inactivating phenolase enzymes,reducing phenolase activity and substrate availability, as wellas pre-treatments by soaking and preconditioning on a basalmedium.
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