Sutrisno Sutrisno
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111

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Keragaman Genetik Inbrida Jagung QPM dan Normal Berbasis Marka Mikrosatelit dan Hubungannya dengan Penampilan Hibrida Marcia B. Pabendon; M. Azrai; M. J. Mejaya; Sutrisno Sutrisno
Jurnal AgroBiogen Vol 4, No 2 (2008): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v4n2.2008.p77-82

Abstract

Information on genetic divergence ofinbred lines and performance of the hybrids developed fromthe lines is a great value in maize hybrid program. A studywas conducted to evaluate genetic diversity of six QPM andfive normal maize inbred lines, to determine the relationshipbetween genetic distance based on SSR markers and thegrain yield of single cross hybrid, and to get informationpromising hybrid from the single cross of QPM hybrid.Twenty four polymorphic primers that covered the 10 maizechromosomes were used to fingerprint the lines, detectingin 94 alleles (average of 3.9 and a range of 2-6 alleles perlocus). Genetic divergences were determined using theJaccard’s similarity coefficient, and a dendrogram wasconstructed using the UPGMA. Cluster analysis divided theinbreds into two clusters that were confirmed by principalcoordinate analysis. Two promising QPM hybrids that arecrossed from different heterotic group were found. Theestimated value of simple correlations (r) of GDs with thegain yield of single cross hybrid was negatif (-0.07). There isa need to conduct more field trials to obtain more accuratecorrelations, particularly in a practical utility for predictingmaize hybrid performance for grain yield.
Pemanfaatan Tanaman Hasil Rekayasa Genetik: Status, Regulasi, dan Metode Deteksi di Indonesia Bahagiawati Bahagiawati; Sutrisno Sutrisno
Jurnal AgroBiogen Vol 3, No 1 (2007): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v3n1.2007.p40-48

Abstract

Application of Genetically Modified Crops: Status, Regulation,and Detection Method in Indonesia. Bahagiawatiand Sutrisno. Global area of transgenic crop was increasetremendously. The number of country accepting of plantingand/or marketing the transgenic crops and its derivativeproducts also become more numberous. However, due toexisting controversy on the benefit and risk, the applicationof transgenic crops was governed by regulations to protectthe consumer and environment from its unwanted effects.There are some international conventions that managingand controlling the uses of these crops, one of them wasCartagena protocol that Indonesia ratified in 2004. Indonesiaalso launched a regulation upon labelling package foodderived from transgenic crops in 1999. To implement eitherthe Cartagena protocol and labelling regulation, Indonesianeeds to increase its capacity to detect the present of thetransgenic crop product either in raw, and proceesed food.This review will discuss about the development of theapplication of transgenic crop and its product globally, andlist of transgenic crops that have been accepted andapproved as safe for human consumption and environment.The regulations upon the application of transgenic crop inIndonesia also be informed. Some metodologies to detectthe presence of the genetically modified food that aregenerally use in some countries also be discussed in thisreview.