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Slamet Slamet
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia Telp. (0251) 8337975; Faks. (0251) 8338820
Agriculture, Biological Sciences & Forestry

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Induksi dan Regenerasi Kalus Jagung yang Ditransformasi dengan Gen CsNitr1-L melalui Penembakan Partikel A. Dinar Ambarwati; Edy Listanto; Slamet Slamet; Umar Umar; Sustiprijatno Sustiprijatno; Sutoro Sutoro
Jurnal AgroBiogen Vol 11, No 1 (2015): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n1.2015.p25-32

Abstract

The success in development of transgenic plants is influenced by the regeneration system. The objective of the study was toassess the response of maize genotypes to regeneration system of organogenesis and embryogenesis, after transformed withCsNitr1-L gene through particle bombardment. Induction and callus regeneration of maize immature embryos of inbred linesUlt:cm.1#, ARC 178-123-112-XB3, and AZ2 were conducted through organogenesis, whereas those inbred lines AZ1, AZ2,P4G19(S)C2.59.3.3.1.3 and P4S3.29.4.4.1 were conducted through embryogenesis somatic. Transformation of CsNitr1-L gene wasdone with the distance of bombardment of 7 cm and 9 cm and calli were then selected using 10 mg/l hygromycin. All explants(100%) of inbred lines Ult:cm.1# formed organogenic callus, while callus formation of ARC 178-123-112-XB3 was 94.3% and AZ2was 60.5%. Ult:cm.1# was the most responsive line to the regeneration of organogenesis and produced 24 green shoots,compared with ARC 178-123-112-XB3 which produced one green shoot and AZ2 that did not produce green shoots. The highestpercentage of embryogenic calli formed through somatic embryogenesis was obtained on inbred lines AZ1 (85.4%) and thelowest was on P4S3.29.4.4.1 (18.9%). Inbred lines AZ1 had the highest percentage of regeneration (50.7%) and produced 62plants, followed by P4G19(S)C2.59.3.3.1.3 that produced 17 plants (2.8%) and P4S3.29.4.4.1 which produced two plants.Preliminary identification on 31 putative transgenic plants through PCR analysis produced 22 plants (70.96%) that contained nptIIgene.
Analisis Molekuler Gen Partenokarpi DefH9-RI-iaaM pada Progeni Tomat Transgenik Saptowo J Pardal; Slamet Slamet; Ragapadmi Purnamaningsih; Endang G. Lestari; Sutini Sutini
Jurnal AgroBiogen Vol 11, No 1 (2015): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n1.2015.p33-40

Abstract

The development of seedless tomato fruits will be more attractive to both consumers and industries. Seedless tomatoes can beproduced through parthenocarpy technology. Artificial parthenocarpy can be induced by conventional crossing, hormoneapplication, or genetic engineering. The development of parthenocarpic tomatoes through genetic engineering has been carriedout by inserting DefH9-iaaM parthenocarpic geneinto tomato genome via Agrobacterium tumefaciens mediated transformation.Sixty putative transgenic tomato lines were produced and three events (OvR1#14-4, OvM2#10-1, and OvM2#6-2) were selectedas the best events. The background of the tomato lines was Oval variety, and based on PCR results, the three selected linescontained DefH9-RI-iaaM in their genome. The objective of this research was to determine the integration of DefH9-RI-iaaMgene in the progenies of three transgenic tomatoes lines using PCR technique. The research was conducted in the laboratoryand Biosafety Containment Facility of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research andDevelopment (ICABIOGRAD). Parental variety, Oval (neither transgenic nor in vitro cultured), and elite line of CL 6046 were usedas control plants. The results indicated that the progenies (T1, T2, and T3) of the three tomato lines contained the insert DefH9-RIiaaMgene.
Co-Authors A. Dinar Ambarwati Edy Listanto Endang G. Lestari Ragapadmi Purnamaningsih Saptowo J Pardal Sustiprijatno Sustiprijatno Sutini Sutini Sutoro Sutoro Umar Umar