Article Per Year (5 Year)
p-Index From 2020 - 2025
0.408
P-Index
This Author published in this journals
All Journal Jurnal AgroBiogen Jurnal Penelitian dan Pengembangan Pertanian Jurnal Penelitian dan Pengembangan Pertanian
Tri Joko Santoso
BB Biogen
Agriculture, Biological Sciences & Forestry Decision Sciences, Operations Research & Management

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

 1 
APLIKASI TEKNIK MOLEKULER UNTUK ANALISIS GENETIK TOMATO LEAF CURL VIRUS Tri Joko Santoso
Jurnal Penelitian dan Pengembangan Pertanian Vol 32, No 4 (2013): Desember 2013
Publisher : Badan Penelitian dan Pengembangan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jp3.v32n4.2013.p141-149

Abstract

Tomato leaf curl virus (ToLCV) merupakan salah satu virus dalamgenus Begomovirus, famili Geminiviridae, yang menyebabkanpenyakit keriting daun pada tomat. Informasi tentang keragamangenetik ToLCV bermanfaat dalam perakitan tanaman tahan. Kemajuandi bidang biologi molekuler telah menghasilkan beberapa teknikyang dapat digunakan untuk analisis genetik Begomovirus. Teknikmolekuler yang banyak diaplikasikan ialah polymerase chainreaction (PCR). Teknik ini sangat sensitif dan spesifik untukmendeteksi Begomovirus pada tingkat DNA. PCR juga dapatdigunakan untuk mengidentifikasi tingkat keragaman genetik virus.Teknik PCR telah digunakan untuk mendeteksi Begomovirus padatomat (ToLCV) dari sentra produksi di Jawa Timur, Jawa Tengah,Jawa Barat, DI Yogyakarta, dan Sumatera. Kombinasi teknik PCRdengan restriction fragment length polymorphism (RFLP) jugadapat digunakan untuk mengidentifikasi keragaman genetik Begomovirus.Selain itu, teknik sekuensing DNA dapat diaplikasikanuntuk mempelajari identitas dan keragaman genetik isolat-isolatToLCV atau anggota Begomovirus lainnya. Analisis sekuen asamamino menunjukkan adanya keragaman genetik dari isolat-isolatToLCV Indonesia. Isolat-isolat tersebut homolog dengan Ageratumyellow vein virus (AYVV). Dengan teknik modifikasi gen (rekayasagenetik) telah berhasil memanfaatkan gen AV1 (coat protein) dariToLCV untuk menghasilkan tanaman tembakau tahan terhadapToLCV. Teknik modifikasi gen memberikan peluang yang besaruntuk mengembangkan tanaman tomat tahan ToLCV dan berperanpenting dalam pembangunan pertanian modern di masa mendatang.
Isolation and Homology Analysis of Alanine Aminotransferase Gene of Barley, Foxtail Millet, Cucumber, and Tomato Atmitri Sisharmini; Aniversari Apriana; Tri Joko Santoso; Bambang Sapta Purwoko; Nurul Khumaida; Kurniawan Rudi Trijatmiko
Jurnal AgroBiogen Vol 16, No 2 (2020): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v16n2.2020.p59-70

Abstract

Overexpression of alanine aminotransferase (AlaAT) gene can improve nitrogen use efficiency (NUE) in plants. The previous isolated AlaAT genes cannot be freely applied to generate NUE plants due to IPR restriction. Therefore, isolation of the gene from targeted monocot and dicot plants is necessary. The objectives of this study were to isolate AlaAT genes from barley, foxtail millet, cucumber, and tomato and analyze their homology to other isolated AlaAT genes in sequence databases (gene bank). Total RNA was isolated from roots of barley, foxtail millet, cucumber, and tomato, and then converted into cDNA using reverse transcription method. The cDNA was then cloned into pGEM®-T Easy plasmid and the verified clones were sequenced. The amino acid sequences were analyzed for their homologies using Clustal Omega software and phylogenetic tree was constructed. The results showed that the amino acids of AlaAT gene from barley was different from AlaAT genes of tomato and cucumber with homology level less than 80%. Phylogenetic tree predicted that AlaAT genes clustered into three groups with AlaAT genes of foxtail millet and barley clustered in one group together with other monocots in group I. AlaAT genes derived from dicots clustered into two groups in which AlaAT gene of tomato clustered in group II, while that of cucumber was in group III. The identity differences of AlaAT gene of tomato and that of cucumber as well as the estimates of the same enzymatic functions can open up enormous opportunities in genetic engineering research for the development of NUE rice.
Molecular and Phenotypic Analyses of Inpari HDB/K15 F2 Lines Containing sd1 Mutant Gene Resulted from Genome Editing Method Clara S. A. Fitriyanti; Suharsono Suharsono Suharsono; Tri Joko Santoso
Jurnal AgroBiogen Vol 16, No 1 (2020): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v16n1.2020.p35-44

Abstract

Inpari HDB variety is resistant to bacterial leaf blight (BLB) disease, but due to its tall stature, the variety is susceptible to lodging. Inpari HDB with semidwarf stature, therefore, is of high interest for lodging resistant performance. sd1 gene encoding GA20ox-2 enzyme is one of the genes responsible for imparting semidwarf stature of rice. In previous study, sd1 mutant rice cv. Kitaake (K15) was developed by using CRISPR/Cas9 technology. The objective of this study was to analyze molecularly and phenotypically F2 lines containing sd1 mutant gene resulted from a cross between Inpari HDB and K15 to develop semidwarfInpari HDB rice variety. Thirty F2 Inpari HDB/K15 lines were analyzed at molecular level using DNA sequencing method together with phenotypic assessment of the lines to verify the integration of sd1 mutant gene. DNA sequencing analysis showed that 9 out of the 30 F2 Inpari HDB/K15 lines were sd1 mutants. The remaining F2 lines contained 17 heterozygotes and 4 nonmutants. All the nine mutant lines demonstrated shorter plant stature and showed more tiller number per plant compared to the nonmutant lines. The sd1 mutant gene in the F2 lines showed pleiotropic effects on panicle number andshowed no effects on other traits, such as flowering time, panicle length, filled and unfilled grain percentages. This study showed the introduction of sd1 mutant gene generated semidwarf Inpari HDB lines. The semidwarf Inpari HDB lines obtained from this research should be further evaluated to confirm their lodging resistant performances.
APLIKASI TEKNIK MOLEKULER UNTUK ANALISIS GENETIK TOMATO LEAF CURL VIRUS Tri Joko Santoso
Jurnal Penelitian dan Pengembangan Pertanian Vol 32, No 4 (2013): Desember 2013
Publisher : Badan Penelitian dan Pengembangan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jp3.v32n4.2013.p141-149

Abstract

Tomato leaf curl virus (ToLCV) merupakan salah satu virus dalamgenus Begomovirus, famili Geminiviridae, yang menyebabkanpenyakit keriting daun pada tomat. Informasi tentang keragamangenetik ToLCV bermanfaat dalam perakitan tanaman tahan. Kemajuandi bidang biologi molekuler telah menghasilkan beberapa teknikyang dapat digunakan untuk analisis genetik Begomovirus. Teknikmolekuler yang banyak diaplikasikan ialah polymerase chainreaction (PCR). Teknik ini sangat sensitif dan spesifik untukmendeteksi Begomovirus pada tingkat DNA. PCR juga dapatdigunakan untuk mengidentifikasi tingkat keragaman genetik virus.Teknik PCR telah digunakan untuk mendeteksi Begomovirus padatomat (ToLCV) dari sentra produksi di Jawa Timur, Jawa Tengah,Jawa Barat, DI Yogyakarta, dan Sumatera. Kombinasi teknik PCRdengan restriction fragment length polymorphism (RFLP) jugadapat digunakan untuk mengidentifikasi keragaman genetik Begomovirus.Selain itu, teknik sekuensing DNA dapat diaplikasikanuntuk mempelajari identitas dan keragaman genetik isolat-isolatToLCV atau anggota Begomovirus lainnya. Analisis sekuen asamamino menunjukkan adanya keragaman genetik dari isolat-isolatToLCV Indonesia. Isolat-isolat tersebut homolog dengan Ageratumyellow vein virus (AYVV). Dengan teknik modifikasi gen (rekayasagenetik) telah berhasil memanfaatkan gen AV1 (coat protein) dariToLCV untuk menghasilkan tanaman tembakau tahan terhadapToLCV. Teknik modifikasi gen memberikan peluang yang besaruntuk mengembangkan tanaman tomat tahan ToLCV dan berperanpenting dalam pembangunan pertanian modern di masa mendatang.
Co-Authors Aniversari Apriana Atmitri Sisharmini Bambang Sapta Purwoko Clara S. A. Fitriyanti Kurniawan Rudi Trijatmiko Nurul Khumaida Suharsono Suharsono Suharsono