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UJI TOKSISITAS EKSTRAK LEMPUYANG GAJAH (Zingiber zerumbet) TERHADAP LARVA UDANG (Artemia salina Leach.) Sintha Suhirman; Hernani Hernani; Cheppy Syukur
Buletin Penelitian Tanaman Rempah dan Obat Vol 17, No 1 (2006): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v17n1.2006.%p

Abstract

Zingiber zerumbet has been used for long time as a traditional medicine to cure dysentri, stoma-chache, and also as carminative. The present work was to study the toxicity of extract of Z. zerumbet used Brine Shrimp Lethality Test (BSLT) method and A. salina as bio indicator. The raw material came from Bogor, Subang, Sumedang and Garut.  The method of quality was used Materia Medika Indonesia (MMI) such ash content, ash insoluble in acid, water and alcohol soluble extractives and also fiber, starch and curcumin contents.  Extracts were made by mace-ration combination with stirring and 3 types of solvents, such as polar (methanol), semi polar (ethyl acetate) and non polar (hexane). The chemical compounds were analyses by thin layer chromatography (TLC) with toluene : ethyl acetate : acetic acid = 8 : 2 : 2 drops as a eluen and 50% sulphuric acid as spray and GCMS. The result showed that there was variation between areas for ash, ash insoluble in acid, water and alcohol soluble extractives. And also in the fiber, starch and curcumin contents were different. The highest of water and, alcohol soluble extractives were from Garut followed by Sumedang. For fiber, starch and curcumin contents were from Sume-dang, Bogor and Subang, respectively. The highest and the lowest yield of extract were from ethyl acetate, Bogor (8.30%) and hexane, Garut (1.49%). The highest and the lowest LD5o of ex-tracts were from ethyl acetate, it was 1.85 ppm and 108.05 ppm, respectively. The result of TLC sho-wed that there were differences in chemical compound separations from extracts between areas, respectively. The identification of chemical compound was showed that the chemical com-ponent of extract which was showed the highest LD5o has the mixture of organic compound such as buthyl hexadecanoat, followed by octadeca-noate acid, hexacosan; and also the component which found in zingiberaceae family such as 3-hydroxy-ll-hyperoxy bisabolan-1,9-dien; 2', 4', 5' trimethoxyphenyl butadien, 7-(4'hyroxyi-3'metho-xyphenyl)-1 hept-4-en-3-on and 1,7 diphenyl-3,5 -heptan dion. 
PENGARUH PUPUK N DAN POPULASI TANAMAN TERHADAP PERTUMBUHAN DAN PRODUKSI JAHE PADA LINGKUNGAN TUMBUH YANG BERBEDA Muhamad Djazuli; Cheppy Syukur
Buletin Penelitian Tanaman Rempah dan Obat Vol 20, No 2 (2009): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v20n2.2009.%p

Abstract

Kondisi lingkungan tumbuh, baik iklim maupun kesuburan lahan, serta teknik budidaya sangat berpengaruh terhadap pertum-buhan dan produktivitas tanaman jahe. Tujuan penelitian untuk mempelajari respon jahe putih besar (Zingiber officinale Rosc.) terhadap pemupukan N dan populasi tanaman pada lingkungan tumbuh yang berbeda. Penelitian dilaksanakan di dusun Cipanas, dengan ke-tinggian 500 m di atas permukaan laut (dpl) dan di dusun Cipicung (800 m dpl) Desa Werasari, Kecamatan Bantarujeg, Kabupaten Majalengka, Jawa Barat. Penelitian dimulai Agustus 2004 sampai dengan Juli 2005, menggunakan Rancangan Acak Kelompok dengan 3 ulangan. Perlakuan terdiri dari enam kombinasi taraf pemupukan N dan populasi tanaman masing masing N1P1 (400 kg Urea/ha - 41.667 tanaman/ha), N1P2 (400 kg Urea/ha - 55.556 tanaman/ha), N2P1 (500 kg Urea/ha - 41.667 tanaman/ha), N2P2 (500 kg Urea/ha-55.556 tanaman/ha), N3P1 (600 kg Urea/ha - 41.667 tanaman/ha), dan N3P2 (600 kg Urea/ha - 55.556 tanaman/ha). Hasil penelitian menunjukkan bahwa perbedaan agroklimat dan kesuburan menghasilkan bobot segar, jumlah anakan dan tinggi tanaman berbeda. Tinggi tanaman, jumlah anakan dan bobot segar di Cipanas adalah 74,69 cm, 16,48 anakan dan 55,89 t/ha, sedangkan di Cipicung masing-masing 42,26 cm, 5,31 anakan dan 7,69 t/ha. Kecukupan hara N pada lahan percobaan khususnya di Cipanas menyebabkan respon tanaman jahe terhadap pemupukan N yang diberikan tidak nyata. Perlakuan populasi yang lebih tinggi sampai 55,556 tanaman/ha mampu meningkatkan produksi jahe sekitar 35 (dari 47,79 t/ha menjadi 64,83 t/ha). 
PENGARUH PUPUK N DAN POPULASI TANAMAN TERHADAP PERTUMBUHAN DAN PRODUKSI JAHE PADA LINGKUNGAN TUMBUH YANG BERBEDA Muhamad Djazuli; Cheppy Syukur
Buletin Penelitian Tanaman Rempah dan Obat Vol 20, No 2 (2009): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v20n2.2009.%p

Abstract

Kondisi lingkungan tumbuh, baik iklim maupun kesuburan lahan, serta teknik budidaya sangat berpengaruh terhadap pertum-buhan dan produktivitas tanaman jahe. Tujuan penelitian untuk mempelajari respon jahe putih besar (Zingiber officinale Rosc.) terhadap pemupukan N dan populasi tanaman pada lingkungan tumbuh yang berbeda. Penelitian dilaksanakan di dusun Cipanas, dengan ke-tinggian 500 m di atas permukaan laut (dpl) dan di dusun Cipicung (800 m dpl) Desa Werasari, Kecamatan Bantarujeg, Kabupaten Majalengka, Jawa Barat. Penelitian dimulai Agustus 2004 sampai dengan Juli 2005, menggunakan Rancangan Acak Kelompok dengan 3 ulangan. Perlakuan terdiri dari enam kombinasi taraf pemupukan N dan populasi tanaman masing masing N1P1 (400 kg Urea/ha - 41.667 tanaman/ha), N1P2 (400 kg Urea/ha - 55.556 tanaman/ha), N2P1 (500 kg Urea/ha - 41.667 tanaman/ha), N2P2 (500 kg Urea/ha-55.556 tanaman/ha), N3P1 (600 kg Urea/ha - 41.667 tanaman/ha), dan N3P2 (600 kg Urea/ha - 55.556 tanaman/ha). Hasil penelitian menunjukkan bahwa perbedaan agroklimat dan kesuburan menghasilkan bobot segar, jumlah anakan dan tinggi tanaman berbeda. Tinggi tanaman, jumlah anakan dan bobot segar di Cipanas adalah 74,69 cm, 16,48 anakan dan 55,89 t/ha, sedangkan di Cipicung masing-masing 42,26 cm, 5,31 anakan dan 7,69 t/ha. Kecukupan hara N pada lahan percobaan khususnya di Cipanas menyebabkan respon tanaman jahe terhadap pemupukan N yang diberikan tidak nyata. Perlakuan populasi yang lebih tinggi sampai 55,556 tanaman/ha mampu meningkatkan produksi jahe sekitar 35 (dari 47,79 t/ha menjadi 64,83 t/ha). 
UJI TOKSISITAS EKSTRAK LEMPUYANG GAJAH (Zingiber zerumbet) TERHADAP LARVA UDANG (Artemia salina Leach.) Sintha Suhirman; Hernani Hernani; Cheppy Syukur
Buletin Penelitian Tanaman Rempah dan Obat Vol 17, No 1 (2006): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v17n1.2006.%p

Abstract

Zingiber zerumbet has been used for long time as a traditional medicine to cure dysentri, stoma-chache, and also as carminative. The present work was to study the toxicity of extract of Z. zerumbet used Brine Shrimp Lethality Test (BSLT) method and A. salina as bio indicator. The raw material came from Bogor, Subang, Sumedang and Garut.  The method of quality was used Materia Medika Indonesia (MMI) such ash content, ash insoluble in acid, water and alcohol soluble extractives and also fiber, starch and curcumin contents.  Extracts were made by mace-ration combination with stirring and 3 types of solvents, such as polar (methanol), semi polar (ethyl acetate) and non polar (hexane). The chemical compounds were analyses by thin layer chromatography (TLC) with toluene : ethyl acetate : acetic acid = 8 : 2 : 2 drops as a eluen and 50% sulphuric acid as spray and GCMS. The result showed that there was variation between areas for ash, ash insoluble in acid, water and alcohol soluble extractives. And also in the fiber, starch and curcumin contents were different. The highest of water and, alcohol soluble extractives were from Garut followed by Sumedang. For fiber, starch and curcumin contents were from Sume-dang, Bogor and Subang, respectively. The highest and the lowest yield of extract were from ethyl acetate, Bogor (8.30%) and hexane, Garut (1.49%). The highest and the lowest LD5o of ex-tracts were from ethyl acetate, it was 1.85 ppm and 108.05 ppm, respectively. The result of TLC sho-wed that there were differences in chemical compound separations from extracts between areas, respectively. The identification of chemical compound was showed that the chemical com-ponent of extract which was showed the highest LD5o has the mixture of organic compound such as buthyl hexadecanoat, followed by octadeca-noate acid, hexacosan; and also the component which found in zingiberaceae family such as 3-hydroxy-ll-hyperoxy bisabolan-1,9-dien; 2', 4', 5' trimethoxyphenyl butadien, 7-(4'hyroxyi-3'metho-xyphenyl)-1 hept-4-en-3-on and 1,7 diphenyl-3,5 -heptan dion.