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All Journal Buletin Penelitian Tanaman Rempah dan Obat BERITA BIOLOGI
sitti fatimah syahid
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OBSERVASI PERTUMBUHAN, HASIL RIMPANG DAN TINGKAT KETAHANAN SOMAKLON JAHE PUTIH BESAR TERHADAP PENYAKIT LAYU BAKTERI DI RUMAH KACA sitti fatimah syahid; otih rostiana; tias arlianti
Buletin Penelitian Tanaman Rempah dan Obat Vol 25, No 2 (2014): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v25n2.2014.77-90

Abstract

Genetic variability in ginger is low, thus it is necessary to obtain new variants. The study was aimed to observe growth, yield and resistance level of ginger somaclones to Ralstonia solanacearum. The experiment was conducted under laboratory and a glass house condition at Indonesian Spice and Medicinal Crops Research Institute, Bogor from January to December 2010. The ginger somaclones used in this study were obtained from somaclonal induction using selection medium Acibenzolar-S-methyl and pathogenic bacteria filtrate at calli stage. The ginger somaclones were then planted at the green house. Four populations of ginger somaclones tested in this experiment were FA, FB, AC1 and FIPLA. All somaclones were inoculated with bacterial wilt disease suspension and then incubated for three months at the green house. The ginger somaclones then were planted in 60 cm x 60 cm polybag filled with 25 kg soil and 1 kg manure. The experiment used direct individual-observation method, without replications. The parameter observed were growth, yield and bacterial wilt disease infection, at 4 and 8 months after planting. The result showed that growth, rhizome yield and bacterial wilt disease infection varied among the somaclones population. Number of tiller, plant height, leaf number, length and width of leaf, stem diameter, rhizome weight and diameter were also varied among populations. Number of propagule of FA and FIPLA was higher than other populations. Visual morphology of somaclones tested were also varied. Based on bacterial wilt disease infection parameter, ginger somaclones population FA, FB, AC1 and FIPLA could be grouped from susceptible to tolerant. 
PENGARUH MEDIA DASAR MS DAN N TERHADAP PERKEMBANGAN EMBRIO 6 SOMATIK PADA KULTUR MERISTEM JAHE (Zingiber officinale Rosc.) Otih Rostiana; Sitti Fatimah Syahid
BERITA BIOLOGI Vol 8, No 5 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i5.1899

Abstract

This study was performed to evaluate the development of somatic embryo from embryogenic calli of ginger meristem culture. Completely randomized design was applied, replicated 4 times. Embryogenic calli from meristem tissue of inner shoot bud of rhizome obtained on MS medium containing 100 mg/L glutamine, 2% sucrose with the addition of 1.0 mg/L 2,4-D and 3.0 mg/L BA, were subjected to proliferation medium, MS and N basal media containing 3% mannitol. Then, transferred into somatic embryo maturation medium, either MS or N basal media supplemented with 6% sucrose. The number of somatic embryos-formed significantly affected by the proliferation medium applied. The highest number of somatic embryos (about 82.0 per 1 g friable calli) was achieved on the MS medium, 4 weeks after incubation. In addition, the optimum growth of embryogenic calli containing somatic embryos was obtained on MS and N medium supplemented with 6% sucrose. There were significantly difference between the media applied (MS and N ) to somatic embryos maturation. The highest number of mature somatic embryos (57.2 embrios) was achieved on the MS medium, 18 days after incubation.
Co-Authors otih rostiana tias arlianti