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Imron Riyadi
Balai Penelitian Bioteknologi Perkebunan Indonesia, Bogor
Biochemistry, Genetics & Molecular Biology

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Pengaruh 2,4-D terhadap Induksi Embrio Somatik Kopi Arabika Imron Riyadi; nFN Tirtoboma
Buletin Plasma Nutfah Vol 10, No 2 (2004): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v10n2.2004.p82-89

Abstract

AbstractDirect induction of somatic embryos in Arabica coffee (Coffea arabica L.) using plant groth regulators (PGR's) has been successful. The concentration and combination of different kinds of PGR's can influence the response and success in embryo induction. An experiment was conducted to determine the optimal concentration of 2.4-D in combination with kinetin for direct induction and proliferation of somatic embryos. The plant material used was Arabica coffee var. Kartika-l originating from The Indonesian Coffee and Cacao Research Institute, Jember. Explants were taken from young leaves of reddish-green in color. Somatic embryos were induced directly on a Murashige-Skoog (MS) standard medium containing 30 g/l sucrose and supplemented with 0, 1, 2, 4, and 8 mg/l 2.4-D in combination with 0.1 mg/l kinetin each. The cultures were incubated in the dark at temperature 26oC and RH +60% for 6 weeks with 10 replications. The results showed that somatic embryogenesis in Arabica coffee was best induced in a culture medium wiyh 2.4-D at 4 mg/l, combination with 0.1 mg/l kinetin. Induction of somatic embryos was achieved at 100% 4 weeks after culture. Three morphological stages of embryo development were identified: globular, early heart, and middle heart. The embryos were of three distinct colors such as, yellowish, yellowish-white, and white. The highest rate of proliferation of somatic embryos was achieved at 2 mg/l, 2.4-D in combination with 0.1 mg/l kinetin averaging 68.53 embryos per explant 6 weeks after subculture.
Isolasi Protoplas Tanaman Kacang Panjang secara Enzimatis Imron Riyadi
Buletin Plasma Nutfah Vol 12, No 2 (2006)
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v12n2.2006.p62-68

Abstract

The technique, kind and concentration of enzyme that were appro-priate and optimum affected the isolation process and rendement result of plant protoplasts. A research was conducted to enhance the protoplast rendements of long bean (Vigna sinensis, L.) that was isolated by enzyme Cellulase RS and Macerozyme R-10 as single and combination in a solution. Concentrations of enzyme were used as much as 2.0-3.0% w/v for Cellulase RS and 0.4-0.6% w/v for Macerozyme R-10. Those solutions contain mannitol 25 mM as osmotycum. Isolation process was done on shaker with 50 rpm (rotation per minute) speed in dark room for 3 hours. Results show that C3 treatment (concentration of Cellulase RS enzyme as much as 3.0% w/v) yielded protoplasts density 17.40 x 105 protoplasts/ g fresh weight of mesophyl and M2 treatment (concentration of Macerozyme R-10 enzyme as much as 0.5% w/v) resulted 17.46 x 105 protoplasts/g. As a whole, the best treat-ment was achieved by C2M2 (combination between Cellulase RS as much as 2.5% and Macerozyme R-10 enzyme as much as 0.5% w/v) which resulted protoplasts density 32.67 x 105 protoplasts/g fresh weight of mesophyl AbstrakTeknik, jenis, dan konsentrasi enzim yang tepat dan optimum berpengaruh dalam proses isolasi dan hasil rendemen protoplas tanaman. Penelitian ini bertujuan untuk meningkatkan rendemen protoplas kacang panjang (Vigna sinensis L.) yang diisolasi dengan enzim Cellulase RS dan Macerozyme R-10 secara individu dan penggabungan dua enzim dalam satu larutan. Konsentrasi enzim yang digunakan adalah 2,0-3,0% b/v untuk Cellulase RS dan 0,4-0,6% b/v untuk Macerozyme R-10. Zat osmotikum yang digunakan adalah mannitol 25 mM. Proses isolasi dilakukan di atas gyotoric shaker dengan kecepatan 50 ppm (putaran per menit) dalam kondisi gelap selama 3 jam. Hasil penelitian menunjukkan bahwa perlakuan C3 (konsentrasi enzim Cellulase RS 3,0% b/v) menghasilkan densitas 17,40 x 105 protoplas/g dan perlakuan M2 (konsentrasi enzim Macerozyme R-10 0,5% b/v) menghasilkan densitas 17,46 x 105 protoplas/g berat segar mesofil daun. Secara keseluruhan, perlakuan terbaik dicapai oleh C2M2 (konsentrasi enzim Cellulase RS 2,5% dan enzim Macerozyme R-10 0,5% b/v) yang menghasilkan densitas 32,67 x 105 protoplas/g berat segar mesofil daun.
Co-Authors nFN Tirtoboma