Yadi Suryadi
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111

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Respon Beberapa Genotipe Kacang Tanah terhadap Penyakit Layu Bakteri (Ralstonia solanacearum) di Rumah Kaca Yadi Suryadi; Sri A. Rais
Buletin Plasma Nutfah Vol 15, No 1 (2009): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/blpn.v15n1.2009.p20-26

Abstract

The objective of the study was to evaluate resistance response of groundnut genotypes against bacterial wilt (Ralstonia solanacearum) in the green house of Biochemistry Department, ICABIOGRAD, Bogor. Ralstonia solanacearum isolate was obtained from groundnut plant showing wilt symptom, collected from Karadenan village (Cibinong). Preparation of bacterial RS inoculum was done using SP (sucrose peptone) medium. Groundnut plants were inoculated by RS isolate using inoculum capacity of 107 cfu/ml. The experiment was arranged in randomized complete block design (RCBD) consisted of seven treatments with four replications, whilst Tupai and Kelinci genotypes were used as resistant and susceptible control check, respectively. The result indicated that groundnut genotypes i.e. ICGV 88262, Local Sindangbarang, PI 203395, ICG 10067, and ICG 3400, showed resistant response against bacterial wilt causing wilt damage of <20%. Bacterial wilt disease could affect reductions of some groundnut phenotypic character. The plant height of resistant groundnut showed relatively tall than that of Kelinci. In addition, it showed the lowest healthy pod yield among the genotypes tested. AbstrakPenelitian bertujuan untuk mempelajari tingkat ketahanan genotipe kacang tanah terhadap penyakit layu bakteri (Ralstonia solanacearum) di rumah kaca Kelompok Peneliti Biokimia BB-Biogen. Isolat R. solanacearum diperoleh dari tanaman kacang tanah yang menunjukkan gejala layu dari Desa Karadenan (Cibinong). Penyiapan dan penyediaan inokulum bakteri R. solanacearum menggunakan media SP (sukrose pepton). Tanaman kacang tanah diinokulasi dengan isolat R. solanacearum dengan kerapatan koloni 107 cfu/ml. Penelitian disusun menggunakan rancangan acak kelompok dengan tujuh perlakuan dan empat ulangan. Untuk pembanding tahan dan rentan masing-masing digunakan genotipe Tupai dan Kelinci. Hasil penelitian menunjukkan bahwa genotipe ICGV 88262, lokal Sindangbarang, PI 203395, ICG 10067, dan ICG 3400 tahan terhadap penyakit layu bakteri dengan nilai kerusakan <20%. Penyakit layu bakteri berpengaruh terhadap penurunan beberapa karakter fenotipik kacang tanah, di mana tanaman genotipe kacang tanah tahan PLB relatif lebih tinggi dibandingkan dengan genotipe Kelinci. Polong bernas Kelinci paling sedikit di antara semua genotipe uji.
SELEKSI JAMUR PATOGEN SERANGGA Beauveria spp. SERTA UJI PATOGENISITASNYA PADA SERANGGA INANG-WALANG (Leptocorisa acuta) Wartono Wartono; Cyntia Nirmalasari; Yadi Suryadi
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2271

Abstract

Rice-stink bug (Leptocorisa acuta Thumb) that attack rice crop often causing heavy damage of panicle of rice as well as decreasing either quantity or quality of grain after harvest. The objective of this research was to select 14 entomopatogenic fungi of Beauveria spp. isolates, collected from rice stink bug (L. acuta) and their pathogenicity assay to insect host L. acuta. We also aimed to study diversity of Beuveria spp. which was isolated from insect host from Situgede, Bogor. The research was conducted at Laboratory, and Green house of Center for Agricultural Biotechnology and Genetic Resources Research and Development, Bogor, in 2014. Result of the study showed that isolates from Situgede Bogor were sucessfully isolated  as Beauveria spp. based on Koch Postulat assay.  The identified fourteen isolates were morphologically confirmed as Beauveria spp. The pathogenicity test was indicated by symptoms and mortality of rice stink bug after inoculation with these entomopathogenic fungi. The Beuveria isolates Stgd2(14)1, Stgd6(14)1, Stgd7(14)2, Stgd8(14)2, and Stgd0113 were the most virulent isolates. Stgd2(14)1 provided the fastest time to kill L. acuta with LT50 values of 6.9 days. The 13 isolates of Beauveria were well amplified by ITS primers. However, no diversity was found among isolates, presumably due its narrow host range tested.