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R. M. Lantican
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Genetic Analysis of Components of Resistance to Philippine Downy Mildew In Maize D. Ruswandi; A. L. Carpena; R. M. Lantican; A. M. Salazar; D. M. Hautea; A. D. Raymundo
Zuriat Vol 13, No 2 (2002)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v13i2.6744

Abstract

The genetics of resistance to Philippine downy mildew in maize (Zea mays L.) caused by Peronosclerospora philippinensis was studied in progeny derived from crosses between the resistant inbred P 345 and susceptible inbred Pi 17 and Pi 23. Plant generations used in this study were the PS (susceptible lines Pi 17 and Pi 23); PR (P 345); extensively F1, F2; F3; BS and BR. Plants at three leaf stages were artificially inoculated and evaluated for components of resistance, namely: disease incidence, disease severity, onset of systemic symptom, area under disease progress curve, and rate of downy mildew development. Analysis of generation means indicate that additive- dominance with epistasis gene effect play important role in all components of resistance to Philippines downy mildew.
Phenological Traits Of Soybean In Correlation With Seed Infection By Cercospora Kikuchii (Matsumoto And Tomoyasu) Gardner D. Ruswandi; R. M. Lantican; R. A. Hautea; M. P. Natural
Zuriat Vol 12, No 2 (2001)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v12i2.6690

Abstract

A study was conducted at Institute of Plant Breeding (IPB), University of thePhilippines at Los Ba~nos (UPLB) from February to July 1997 to determine any relationship among various phonological traits with purple seed stain on soybean caused by C. kikuchii. Thirty genotypes that showed different levels of resistance and susceptibility under the 1996 natural field experiment were used in the study. Results suggest that phonological traits in R7-1-R7-2, R7-1-R7-3, R7-1-R8, R7-2-R8 and R7-3-R8 can serve as selection criteria for developing resistance to C. kikuchii.
Components of Quantitative Resistance to Philippine Downy Mildew In Maize D. Ruswandi; A. D. Raymundo; R. M. Lantican; D. M. Hautea; A. M. Salazar; A. L. Carpena
Zuriat Vol 13, No 1 (2002)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v13i1.6719

Abstract

Resistance to Philippine downy mildew (Peronosclerospora philippinensis) was observed in a BC1F2 population developed from a cross between the susceptible inbred line Pi 23 and the resistant inbred line P 345. Five components of resistance namely, disease incidence, disease severity, onset of systemic symptoms, the-area- underdisease-progress- curve (AUDPC), and rate of downy mildew development affected host resistance reactions. All components were significantly correlated. The adult plant resistant reaction was evident in resistant progenies showing prolonged onset of systemic symptoms. Transgressive segregation that exceeded the high and low parent values was also observed for all components of quantitative resistance to Philippine downy mildew.
Quantitative Trait Loci Mapping Of Philippine Downy Mildew Resistance Gene In Maize (Zea Mays L.) D. Ruswandi; D. M. Hautea; A. L. Carpena; R. M. Lantican; A. M. Salazar; A. D. Raymundo
Zuriat Vol 13, No 1 (2002)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v13i1.6724

Abstract

An experiment to locate QTL conferring resistance against Peronosclerospora philippinensis causing Philippine downy mildew was observed in a BC1F2 population developed from a cross between the susceptible inbred line Pi 23 and the resistant inbred line P 345. Phenotypic data were collected in Los Baños, UPLB for disease incidence. A total of four regions were associated with disease incidence. Some of QTL associated with disease incidence have been reported previously, and the remaining QTL are described here for the first time. The results on QTL analysis using molecular markers also agree with the previous study using classical generation mean analysis of the cross combination Pi 23 x P 345.
Map Construction Of Simple Sequence Repeat Developed For Introgression Philippine Downy Mildew Resistance Gene In Maize D. Ruswandi; D. M. Hautea; A. M. Salazar; R. M. Lantican; A. L. Carpena; A. D. Raymundo
Zuriat Vol 12, No 2 (2001)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v12i2.6692

Abstract

Simple sequence repeats (SSRs) is an important DNA markers for mapping plant genomes particularly in mapping quantitative trait loci (QTL). To identify and map SSR markers for introgression resistance gene against Peronosclerospora philippinensis in maize, the parental materials (P 345 and Pi 23) together with 142 genotypes of BC1F1 of Pi 23 ×P 345 population were used. A total of 50 SSR markers were genotyped. However, only thirty-three markers were integrated into a molecular map together with RFLP and RGA markers. A molecular map of SSR was successfully constructed together with RFLP and RGA into 10 chromosomes. It covered a total map length of 1822 cM with an average distance of 18.22 cM between markers.
Co-Authors A. D. Raymundo A. L. Carpena A. M. Salazar D. M. Hautea D. Ruswandi M. P. Natural R. A. Hautea