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C. Talib
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Efektivitas Catatan Test Day untuk Evaluasi Genetik Produksi Susu pada Sapi Perah H. Indrijani; A. Anang; R. R. Noor; C. Talib
Zuriat Vol 14, No 1 (2003)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v14i1.6818

Abstract

Penelitian ini bertujuan untuk mempelajari efektivitas catatan Test day dengan model regresi tetap untuk evaluasi genetic produksi susu pada sapi perah berdasarkan laktasi satu dan dua. Data produksi susu berasal dari sapi perah Frisian Holstein periode laktasi satu dan dua dari tahun 1989–2000 di PT. Taurus Dairy Farm Sukabumi. Catatan Test day sebanyak 7503 buah berasal dari 456 ekor sapi betina, 40 pejantan dan 342 ekor induk. Model yang digunakan adalah Model Regresi Tetap, varian komponen diduga dengan Animal Model dengan Restricted Maximum Likelihood (REML) dan Nilai Pemuliaan dengan Best Linier Unbiased Prediction (BLUP). Hasil analisis menunjukkan hari test ke-35 (TD2) merupakan puncak produksi. Pengaruh musim terhadap produksi susu Test day mempunyai pola yang kurang jelas. Ragam genetik aditif pada laktasi dua menurun sejalan dengan penambahan catatan Test day sampai dengan Test day ke-3, setelah itu meningkat sampai akhir laktasi. Dugaan nilai heritabilitas mengikuti pola yang sama dengan ragam genetik. Evaluasi genetic berdasarkan laktasi satu penuh yang ditambah dengan satu catatan Test day pada laktasi dua sudah cukup untuk mengevaluasi seluruh ternak, sedangkan untuk mengevaluasi pejantan, evaluasi harus diperpanjang sampai 3 catatan Test day pada laktasi dua.
Extraction and isolation of Ovine Pregnancy-Associated Glycoprotein (ovPAG) from cotyledon placenta of Garut sheep E.T. Setiatin; D. Sajuthi; B. Purwantara; C. Talib
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 3 (2009): SEPTEMBER 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.806 KB) | DOI: 10.14334/jitv.v14i3.342

Abstract

Pregnancy-associated glycoprotein (PAG) structurally related to aspartic protease, expressed in the outer epithelial cell layer (trophectoderm) of ungulate placenta. Ovine PAG (ovPAG) synthesized by mono- and binucleic trophoblast before complete implantation at Day 14-15. Of this, ovPAG could be used as a marker for early pregnancy. The objective of study was to extract and isolate PAG from placenta of Garut Sheep collected at term and to characterize their molecular weight. The procedures included extraction of protein at neutral pH (cotyledon was thawed, minced, added PBS, blended and centrifuged), acidic (H3PO41M, pH 4,5; centrifuged) and ammonium sulfate (40% and 80% (NH4)SO4, centrifuged) precipitation; gel filtration (Sephadex-G75), anion exchange chromatography (DEAE- cellulose). Cotyledon extract was subjected to Sephadex-G75 and DEAE cellulose, and their fractions were measured their absorbances. Absorbances of Sephadex-G75 and DEAE fractions at peak were assayed for protein concentration (Bichinconinic protein assay). Continuously, these fractions were subjected to monogel SDS-PAGE and stained by Commassie Brilliant Blue. It was four different molecular weights isolating from cotyledon of Garut Sheep, namely 68.8, 36.04, 32.39 and 12.18  kDa. However, after chromatography anion exchange (DEAE-cellulose), only  three bands exist, those were  71,67;  33,64 and 30,86 kDa. Key words: Garut sheep, Pregnancy-Associated Glycoprotein (PAG), Cotyledone, SDS-PAGE, DEAE-cellulose
Co-Authors A. Anang B. Purwantara D. Sajuthi E.T. Setiatin H. Indrijani R. R. Noor