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UJI DAYA HAMBAT EKSTRAK BAWANG PUTIH (Allium sativum) TERHADAP BAKTERI (Staphylococcus Aureus) Yusril Ilham Fahmi; Ana Andriana; Diani Sri Hidayati
JURNAL KEDOKTERAN Vol 4 No 2 (2019)
Publisher : Fakultas Kedokteran Universitas Islam Al-Azhar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.265 KB) | DOI: 10.36679/kedokteran.v4i2.109

Abstract

Mikroorganisme alami yang ada dalam tubuh manusia disebut mikroorganisme normal atau flora normal, namun dalam keadaan tertentu dapat bersifat patogen dan menimbulkan penyakit infeksi salah satunya Staphylococcus Aureus. Bakteri ini sering resisten terhadap berbagai jenis obat sehingga mempersulit pemilihan antimikroba yang sesuai untuk terapi. Bawang putih (Allium Sativum) mempunyai bahan aktif yaitu alicin, flavonoid, ajoene, minyak atsiri dan saponin yang mempunyai efek antimikroba. Tujuan penelitian ini adalah mengetahui daya hambat ekstrak bawang putih (Allium Sativum) terhadap pertumbuhan bakteri Staphylococcus aureus. Penelitian ini merupakan jenis penelitian True Experiment dengan rancangan Posttest dengan Kelompok Kontrol (Posttest Only Control Group Design), menggunakan Rancangan Acak Lengkap (RAL) dengan 24 perlakuan dan 4 kali ulangan. Sampel yang digunakan dalam penelitian ini adalah bakteri Staphylococcus aureus yang diberi perlakuan ekstrak bawang putih (Allium Sativum) dengan konsentrasi 250 µg/mL, 500 µg/mL, 750 µg/mL, 1000 µg/mL, 2 kelompok kontrol terdiri dari kontrol positif ciprofloksasin dan kontrol negatif aquades steril. Daya hambat diperoleh berdasarkan pengukuran zona hambat dan data yang diperoleh dari analisis menggunakan uji One Way Anova. Hasil pengukuran zona hambatan bakteri Staphylococcus aureus pada masing-masing konsentrasi berturut turut adalah, konsentrasi 250 µg/mL sebesar 6.5 mm; 500 µg/mL,13.3 mm; 750 µg/mL, 15 mm dan 1000 µg/mL, 17.8 mm. Pada konsentrasi yang paling tinggi menghasilkan daerah zona hambat yang terlebar. Hasil uji One Way Anova diperoleh nilai signifikan yakni 0,008 lebih kecil dari p value: 0,05 sehingga dapat dinyatakan bahwa konsentrasi ekstrak bawang putih (Allium Sativum) dapat mempengaruhi pertumbuhan bakteri Staphylococcus Aureus.
UJI TOKSISITAS EKSTRAK KELINCI LAUT (Aplysia parvula) DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT) Ana Andriana
JURNAL KEDOKTERAN Vol 1 No 1 (2015)
Publisher : Fakultas Kedokteran Universitas Islam Al-Azhar

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Abstract

Kelinci laut Aplysia parvula diekstrak dengan metode maserasi menggunakan campuran metanol:diklorometana (2:1). Ekstrak diklorometana dipartisi menggunakan etil asetat:air (3:2). Ekstrak kasar etil asetat dipisahkan dengan kromatografi kolom menggunakan sistem elusi gradien dari n-heksana, kloroform, etil asetat dan metanol. Ekstrak kasar etil asetat dan fraksi dari kromatografi kolom diuji toksisitasnya menggunakan metode Brine Shrimp Lethality Test (BSLT). Hasil uji toksisitas menunjukkan Nilai Lethal Consentration 50 (LC50) ekstrak kasar etil asetat adalah 69,18 µg/mL, sedangkan fraksi I, II, III, IV dan fraksi V hasil kromatografi kolom menunjukkan Nilai LC50 masing-masing sebesar 330,37; 104,47; 295,80; 335,74 dan 223,87 µg/mL.
UJI DAYA HAMBAT EKSTRAK DAUN SIRSAK (Annona muricata Linn) TERHADAP BAKTERI Escherichia coli SECARA IN VITRO Luh Made Ionnandha; Musyarrafah; Sabariah; Ana Andriana
Nusantara Hasana Journal Vol. 2 No. 11 (2023): Nusantara Hasana Journal, April 2023
Publisher : Yayasan Nusantara Hasana Berdikari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59003/nhj.v2i11.827

Abstract

Soursop plant (Annona muricata Linn) is a type of traditional medicinal plant. Soursop plants are widely studied because the content of fruit and leaves can provide many benefits for society. Many people use parts of the soursop plant to cure various diseases such as diarrhea caused by Escherichia coli bacteria. Soursop leaf extract (Annona muricata Linn) is known to contain flavonoids, alkaloids, and saponins, tannins, acetogenins which can inhibit bacterial growth. The purpose of this study was to determine the inhibition of soursop leaf extract (Annona muricata Linn) towards against the growth of Escherichia coli bacteria. This research was conducted in the Research laboratory of the Faculty of Medicine of Al-Azhar Islamic University using the method of extrasion (maceration) with ethanol solvent. The design used in this study was a Complete Randomized Design (RAL) with 4 treatment groups and 2 controls (positive ciprofloxacin and negative aqudest). The sample data obtained was then analyzed using SPSS version 23. The results of this study obtained the diameter of the inhibitory zone in 4 treatment groups. The largest inhibitory zone dimater is 10,25 mm at a concentration of 100%, and the lowest inhibitory power is 2 mm at a concentrationof 25%. Based on the results of the analysis carried out, a significant difference was obtained between the treatment groups with a value (p<0.05). Soursop leaf extract (Annona muricata Linn) has an inhibitory power against the growth of Escherichia coli bacteria.
EFEKTIVITAS KOMBINASI EKSTRAK DAUN JERUK NIPIS DAN PERASAN BUAH JERUK NIPIS (Citrus aurantifolia) TERHADAP MORTALITAS KUTU RAMBUT (Pediculus humanus var capitis) I Putu Dion Kumara Yadnya; Fahriana Azmi; Ana Andriana; Angelica Vanini Winata Taufiq
Nusantara Hasana Journal Vol. 2 No. 11 (2023): Nusantara Hasana Journal, April 2023
Publisher : Yayasan Nusantara Hasana Berdikari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59003/nhj.v2i11.828

Abstract

Diseases that tend to occur in tropical climates such as Indonesia are generally infectious diseases, one of which is pediculosis capitis. Risk factors Pediculosis capitis mainly affects young children and women and quickly spreads in a crowded living environment and conditions of poor personal hygiene. The increase in Pediculosis capitis infestations in various countries is due to resistance to the use of synthetic pediculosides. One plant that has potential as a natural insecticide is lime (Citrus aurantifolia). Lime contains saponins, flavonoids, and limonoida compounds. The objective of this study is to determine the effectiveness of a combination of lime leaf extract and lime juice as the most effective natural insecticide for head lice. The method used in this study was true experiment with the design of the Post test with the Control Group (Posttest Only Control Group Design) and Completely Randomized Design (CRD) with 5 treatment groups combination of lime leaf extract and lime juice in a ratio of 1:0, 1:3, 1:1, 3:1, dan 0:1. Results of statistical tests obtained were 0.000 (p <0.05) meaning that there was an effectiveness of the combination of lime leaf extract and lime juice (Citrus aurantifolia) on the mortality of head lice (Pediculus humanus var capitis). The combination of lime leaf extract and lime juice (Citrus aurantifolia) is effective against head lice (Pediculus humanus var capitis) mortality. The most effective combination concentration was 25% lime leaves with 75% lime fruit, 100% lime leaf and 100% lime fruit concentration resulting in 100% head lice mortality 2 hours after the intervention.