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Nita Etikawati
Biology Study Program, Faculty Of Mathematics And Natural Science, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Kentingan, Surakarta 57126

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Optimasi Sistem Transformasi Gen Xiloglukanase Pada Eucalyptus pellita F. Muell Menggunakan Agrobacterium tumefaciens Sri Hartati; Andriyani Puspitaningrum; Nita Etikawati; Enny Sudarmonowati
Bioscience Vol 2, No 1 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (276.53 KB) | DOI: 10.24036/020182110044-0-00

Abstract

Eucalyptus pellita is a type of woody plant that is widely used as raw materials of pulp and paper so that the need for wood from this type of plant is increasing. Improvements in wood quality such as cellulose deposition and increased growth rates are needed to support the supply of raw materials for the pulp and paper industry. One technology to change the composition of wood is the modification of plant cell walls through the transformation of xylolukanase gene which in other plants such as Populus alba and Acacia mangium have been shown to increase cellulose deposition and spur growth. The purpose of this study was to obtain an efficient xyloglucanase transformation method in E. pellita using Agrobacterium tumefaciens. Sprouts E. pellita 006 and 06A with different ages of 8 and 15 days are used as plant material for transformation. The sonication treatment of the sprouts prior to the transformation was also applied to determine the effect on transformation efficiency. Transformation is done by soaking the seeds that have been through the treatment of sonication and without sonication on the suspension of Agrobacterium carrying plasmid pAa XEG300 and subsequently grown on the selection medium. Sprouts E. pellita 006 aged 15 days without sonication treatment showed the highest percentage of regeneration in the selection media that is equal to 72%. Gene integration testing through DNA amplification with specific primers showed a ribbon of xyloglucanase with a size of 709 bp.
Shelf Life and Secondary Metabolite Content of Sweet Potato (Ipomoea batatas L.) Lam. Coated with Chitosan Coating at Low Temperature Storage Widya Mudyantini; Suranto Suranto; Solichatun Solichatun; Nita Etikawati; Ari Pitoyo; Suratman Suratman; Tanjung Ardo
agriTECH Vol 43, No 1 (2023)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.61099

Abstract

Sweet potato (Ipomoea batatas (L.) Lam.) has diverse varieties with different secondary metabolite content. Postharvest treatment with low-temperature storage and chitosan coating is expected to expand the shelf life of sweet potatoes. The combination of these treatments will affect the secondary metabolite content of diverse sweet potato varieties. Therefore, this study aims to observe the secondary metabolite content and shelf life extension of 3 sweet potato varieties after coating with chitosan and low-temperature storage. A completely randomized design (CRD) was used with a three-factors experiment. The first factor was chitosan concentration at 0, 10, 15, and 20 g/L, the second was storage room temperature at 25, 15, and 5 °C, while the third was the color of sweet potato varieties namely white, purple, and orange from Tembakur and Mendut varieties. Meanwhile, the control group was tubers without chitosan coating at a storage temperature of 25 °C. Each treatment had five replications and the parameters assessed were changes in wet weight, hardness, respiration, the total chlorophyll level, carotenoid, vitamin C, reducing sugar, and the level of flavonoid. Data were analyzed with Analysis of Variance and then continued with Duncan's Multiple Range Test at a significance level of 5%. The result showed that low-temperature storage combined with chitosan coating affected the shelf life of sweet potatoes. Overall, the best storage temperature was 15 °C, indicated by the highest residual secondary metabolite and the most extended shelf life. The 5°C treatment decreased oxygen consumption during storage, as indicated by a low respiration rate. However, this storage temperature caused a chilling injury and culminated in the shorter shelf life of all examined sweet potatoes. The best coating was achieved by chitosan 15 g/L, indicated by the capability to coat sweet potato surface and maintain the high content of all targeted chemical components. The results also revealed that 20 g/L chitosan concentration is not practical for coating due to its in elasticity and the potential to create a crack in the coating layer.