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Dita Widayanti Sawitri
Biology Departement, Sains and Technology Faculty, Airlangga University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Growth Response Test on Potential Indigenous Bacteria to Degrade Naphthalene and Phenanthrene on various pH Alfi Rizca Hardianti; Helga Lusiana; Dita Widayanti Sawitri
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (545.79 KB) | DOI: 10.24036/0201932105264-0-00

Abstract

This study aims to understand the growth response of indigenous bacteria originating from oil sludge in Dumai which has the potential as Naphthalene and Fenantren degrading agents based on variations in substrate pH. The isolates used in this study were Isolate A, Isolate E, and Isolate F as a result of isolation from oil sludge in Dumai. Based on the results of the growth response test on various concentration in previous studies, we chose E isolates to be tested for its degradation ability. The bacterial growth response data was obtained based on OD measurements every 24 hours using a spectrophotometer and TPC at 72 hours using Nutrient Agar media. Based on these data, we determined the optimum pH for indigenous bacterial growth potential to degrade naphthalene or phenanthrene. In all variations of pH, bacterial isolate E experienced growth. This shows that bacterium E can use naphthalene and fenantren at normal pH range (pH 5-9).
Co-Authors Alfi Rizca Hardianti Helga Lusiana