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Haliani Haliani
Tadulako University
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INDUKSI KALUS BAWANG MERAH (ALLIUM ASCALONICUM L.) LOKAL PALU PADA MEDIUM MS DENGAN PENAMBAHAN 2,4-D (2,4-ASAM DIKLOROPENOKSI ASETAT) DAN AIR KELAPA Sorentina, Melisa S.M.; Haliani, Haliani; Muslimin, Muslimin; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 2, No 2 (2013): Volume 2 Number 2 (August 2013)
Publisher : Univ. Tadulako

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Abstract

Shallot c.v. Local Palu (BML-Palu) is one of importance agricultural commodities in Palu and surrounding area of Central Sulawesi. Unfortunately, in agricultural system, it?s facing difficulties in seed propagation. However, tissue culture approach was a potential technology which can be applied in order to overtake the problem. Our study was an early step in the application of this technology. We tried to find out the best combination of 2,4-D and coconut water in MS based medium, which suitable for callus induction. This experimental study was based on Completely Randomized Design (CRD), with 4 treatments, 3 replications, and 3 shallot explants on each unit. The treatments were PK1 (MS0 + 1 ppm 2,4-D + 10% coconut water), PK2 (MS0 + 1,5 ppm 2,4-D + 10% coconut water), PK3 (MS0 + 2 ppm 2,4-D + 10% coconut water), and PK4 (MS0 + 2,5 ppm 2,4-D + 10% coconut water). Out came of this research showed that all treatments, except PK2, were able to induce callus formation up to 80.55% of total explants. Based on the observed parameters i.e. time of Callus emerging, frecuency of explants producing callus, callus morphology and callus cell observation; PK3 was the best medium in callus induction and growth. Callus on this medium was intermediate-type, white in color, active in  cell propagation, also uniform in size. While PK2 was fail to induce the callus due to overproduction of secondary metabolite.
ORGANOGENESIS TANAMAN BAWANG MERAH (ALLIUM ASCALONICUM.L) LOKAL NAPU SECARA IN VITRO PADA MEDIUM MS DENGAN PENAMBAHAN IAA DAN BAP Utami, Felma Tri; Haliani, Haliani; Muslimin, Muslimin; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 2, No 2 (2013): Volume 2 Number 2 (August 2013)
Publisher : Univ. Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.504 KB)

Abstract

The aims of this study were to find out the optimum combination of IAA and BAP hormones in MS based medium, which suitable for promoting in vitro-organogenesis. In vitro experiment on inducing organogenesis of Shallot c.v. Local Napu showed that combination of 0.01 ppm IAA and 1 ppm BAP, it was the best combination on MS based medium for that organogenesis. These result was based on the measurements over several parameters, including: period of emerging shot and root, precentage of explant growth, number of roots, number of leaves, number of stomata, also chlorophil content of planlet leaves. This data suggesting that MS medium containing 0.01 ppm IAA and 1 ppm BAP was a potential medium to be used on propagation of shallot c.v. Local Napu.
Co-Authors Ali Djamhuri Felma Tri Utami I Nengah Suwastika Melisa S.M. Sorentina