Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal MARINA CHIMICA ACTA
M Natsir Djide
Faculty of Pharmacy, Hasanuddin University
Biochemistry, Genetics & Molecular Biology

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
MOLECULAR CHARACTERIZATION OF GENE 16S rRNA MICRO SYMBIONTS Ismail Marzuki; Alfian Noor; Nursiah La Nafie; M Natsir Djide
Marina Chimica Acta Vol. 16 No. 1 (2015): Enzyme from Bacteria and Coral Age
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (471.284 KB) | DOI: 10.20956/mca.v16i1.956

Abstract

Molecular characterization studies have been conducted 16S rRNA gene micro symbiont of sponge origin Melawai Beach, Balikpapan in East Kalimantan. Objective analysis of histomorphological research, isolation-purification, molecular characterization of micro-symbiont genes in order to search symbiont bacteria that can live in extreme environments contaminated hydrocarbon waste. The research method that morphological identification, isolation-purification and molecular characterization of the 16S rRNA gene with Chain Reaction Polymerization method. The results of histo-morphological analysis concluded sponge samples with species of Callyspongia sp Isolation and purification mikro symbionts of sponge obtained 2 (two) isolates. Characteristics of Isolates 1; spherical shape, colonize and creamy, while isolates 2; jagged shape, oval and white colonies. Molecular characterization of the 16S rRNA gene by PCR, Bacillus subtilis strain BAB-684 identification for isolates one is the number of nucleotide pairs reached 899 bp and the degree of similarity in GenBank reached 89% homologous, while the second is a Bacillus flexus strain PHCDB20 isolates the number reached 950 bp nucleotide pairs with the degree of similarity in GenBank reached 99% homologous.Keywords: Callyspongia sp, micro-symbionts, characterization, gene Molecules
Co-Authors Alfian Noor Ismail Marzuki Nursiah La Nafie