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NAZARUDDIN NAZARUDDIN
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Energy Engineering Immunology & microbiology Medicine & Pharmacology Neuroscience Physics

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Optical pH sensor based on polyelectrolyte complex (PEC) pectin-chitosan/methanol anthocyanin extract of Catharanthus roseus for a new optical urea biosensor development NURHAYATI NURHAYATI; EKA SAFITRI; KHAIRI SUHUD; NAZARUDDIN NAZARUDDIN; BINAWATI GINTING; SUSILAWATI SUSILAWATI; SITTI SALEHA; MURNIANA MURNIANA; SALSABILLA LATANSA NAZARUDDIN
Jurnal Natural Volume 21 Number 3, October 2021
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1244.489 KB) | DOI: 10.24815/jn.v21i3.22851

Abstract

Construction of optical pH sensor as a new platform optical urea biosensor based on polyelectrolyte complex (PEC) pectin-chitosan membrane and total phenolic (anthocyanin) of Tapak Dara flower (Catharanthus roseus) has been successfully carried out. The anthocyanin was extracted by a maceration method for 72 hours using methanol, and a total extract yield was 21.56% or 1.803 mg/L. Flavonoid and phenol tests showed positive results indicated by the formation of red and black colours. The anthocyanins showed maximum absorption at 578 nm for phosphate buffer and 575 nm for tris HCl buffer solution. The sensor fabrication was performed using a mixture of pectin and chitosan solutions with a ratio of 3:7. The anthocyanin was mixed into the solution with various concentrations. The sensor has an optimum sensitivity at the anthocyanin concentration of 0.05 mg/L (phosphate buffer) in the pH range of 7.0-9.5 and 0.025 mg/L (Tris HCl buffer) with a narrower pH range of 6.0-7.5. This sensor produced higher sensitivity, a wider linear range, and good linearity when it was exposed in 0.03M PBS. Reproducibility test with a relative standard deviation percentage (% RSD) was 9.20. The sensor showed a stable response after 5 minutes exposed to PBS solution, and it can be used to measure pH within the 20th day. The optimized optical pH sensor has been successfully developed as a urea optical biosensor by immobilizing urease on its surface. The biosensor showed a linear response in a series of 10-1-10-10 M urea concentrations and has good linearity.
Isolation and characterization of polyhydroxyalkanoate (PHA) producing bacteria isolate from landfill land of Kampung Jawa Banda Aceh MAZWAN MAZWAN; FEBRIANI FEBRIANI; NAZARUDDIN NAZARUDDIN
Jurnal Natural Volume 23 Number 1, February 2023
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v23i1.29746

Abstract

The production of biodegradable plastic from microorganisms has great potential as a substitute for conventional plastic. This study aims to isolate bacterial strains capable of polyhydroxyalkanoates (PHAs) production from the Kampung Jawa landfill land (KJLL) and characterize biopolymers. The bacterial strains were able to produce PHA using a mineral salt medium (MSM) with glucose as a carbon source. The qualitative screening of PHA-producing bacteria was conducted by Sudan Black and Nile Red. Of the 64 bacteria strains, only 41 were able to accumulate PHA in Sudan Black and Nile Red. The results showed that one bacteria the Coccobacillus strain had the highest color intensity for further characterization of PHA. The characterization of PHA by Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) showed a melting temperature (Tm) of 101.54°C. X-ray diffraction (XRD) analysis revealed a crystalline structure with an index crystallinity (ICr) value of 15.82% for PHA. The results of the analysis proved that PHA was produced by bacteria isolate. This study suggests that this is the first report of the bacteria from the Kampung Jawa landfill producing PHA with good characteristics and potential biotechnology applications. 
Optical pH sensor based on polyelectrolyte complex (PEC) pectin-chitosan/methanol anthocyanin extract of Catharanthus roseus for a new optical urea biosensor development NURHAYATI NURHAYATI; EKA SAFITRI; KHAIRI SUHUD; NAZARUDDIN NAZARUDDIN; BINAWATI GINTING; SUSILAWATI SUSILAWATI; SITTI SALEHA; MURNIANA MURNIANA; SALSABILLA LATANSA NAZARUDDIN
Jurnal Natural Volume 21 Number 3, October 2021
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v21i3.22851

Abstract

Construction of optical pH sensor as a new platform optical urea biosensor based on polyelectrolyte complex (PEC) pectin-chitosan membrane and total phenolic (anthocyanin) of Tapak Dara flower (Catharanthus roseus) has been successfully carried out. The anthocyanin was extracted by a maceration method for 72 hours using methanol, and a total extract yield was 21.56% or 1.803 mg/L. Flavonoid and phenol tests showed positive results indicated by the formation of red and black colours. The anthocyanins showed maximum absorption at 578 nm for phosphate buffer and 575 nm for tris HCl buffer solution. The sensor fabrication was performed using a mixture of pectin and chitosan solutions with a ratio of 3:7. The anthocyanin was mixed into the solution with various concentrations. The sensor has an optimum sensitivity at the anthocyanin concentration of 0.05 mg/L (phosphate buffer) in the pH range of 7.0-9.5 and 0.025 mg/L (Tris HCl buffer) with a narrower pH range of 6.0-7.5. This sensor produced higher sensitivity, a wider linear range, and good linearity when it was exposed in 0.03M PBS. Reproducibility test with a relative standard deviation percentage (% RSD) was 9.20. The sensor showed a stable response after 5 minutes exposed to PBS solution, and it can be used to measure pH within the 20th day. The optimized optical pH sensor has been successfully developed as a urea optical biosensor by immobilizing urease on its surface. The biosensor showed a linear response in a series of 10-1-10-10 M urea concentrations and has good linearity.
Co-Authors BINAWATI GINTING EKA SAFITRI Febriani Febriani Firaihanil Jannah Ilham Maulana KHAIRI SUHUD Lelifajri Lelifajri MAZWAN MAZWAN MURNIANA MURNIANA Nasrullah Idris Nor Diyana Md Sani NURHAYATI NURHAYATI NURHAYATI NURHAYATI NURHAYATI NURHAYATI Rahmadi Rahmadi Rinaldi Idroes SALSABILLA LATANSA NAZARUDDIN SITTI SALEHA Supriatno Supriatno SUSILAWATI SUSILAWATI