Garuda - Garba Rujukan Digital

Purification and Partial Characterization of α-Amylase Produced by a Thermo-Halophilic Bacterium Isolate PLS 75 Iqbalsyah, Teuku M.; Fajarna, Farah; Febriani, Febriani
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Bio-based industries require stable enzymes in a broad range of environmental conditions. Extremophiles have attracted more interests as the source of such enzymes, one of which is α-amylase. This study aimed to purify and characterize α-amylase produced by a thermo-halophilic bacterium PLS 75 isolated from underwater fumaroles. Ammonium sulfate precipitation results showed that the highest specific α-amylase activity (21.7 U/mg) obtained at 40-60% saturation level, with a purity of 7.7-fold of the crude extract with 16.2% yield. Further purification using DEAE Sepharose column chromatography increased the enzyme purity 11.1-fold of the crude extract with 7.1% yield. Specific activity after column chromatography purification was 31.3 U/mg. The pure enzyme had a low molecular weight of 14 kDa. The enzyme showed the highest activity at 80 °C and pH 5. The activity increased to 126% when in methanol, while decreased when in ethyl acetate and chloroform. The characteristics of α-amylase with low molecular weight, which was active in acidic condition, stable in polar and non-polar solvents, may be used for for specific industrial needs.

MUTU SENSORI KOPI LUWAK ASAL DATARAN TINGGI GAYO Murna Muzaifa; Yusya Abubakar; Febriani Febriani; Amhar Abubakar; Dian Hasni
AGROINTEK Vol 15, No 3 (2021)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v15i3.9604

Indonesia is rich in specialty coffee. Some of the specialty coffees from Indonesia are Lintong coffee, Mandailing coffee, Gayo coffee, Toraja coffee and others. Luwak coffee with unique processing is categorized as specialty coffee. Luwak coffee is known as one of the special and most expensive coffees in the world. The Gayo Highlands, as one of the best and largest arabica coffee producing areas in Indonesia, also produces civet coffee. It is suspected that the quality of Gayo arabica civet coffee is also very good. This study aims to analyze the sensory quality of civet coffee from the Gayo Highlands. The results showed that civet coffee from the Gayo Highlands has various sensory. The quality of the cupping score is very good 83.75 (very good) -85.75 (excellent) with the most dominant aromas being nutty, fishy, chocolaty and herby

Estimasi Dosis Radiasi 210Po pada Ikan Laut Konsumsi dari Perairan Banda Aceh Risa Chintia Balqis; Febriani Febriani; Murdahayu Makmur
Jurnal Kelautan Tropis Vol 24, No 3 (2021): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v24i3.10219

The main idea of this study is to calculate 210Po activity in marine biota from Banda Aceh waters and calculate dose estimates for marine biota consumers. Polonium analysis was carried out for Kaka Tua fish, Layur fish, Talang-talang fish, Kawet fish, and sharks using alpha spectrometer. The highest 210Po activity was found in sharks at 16.90 Bq/kg and Kaka tua fish at 16.24 Bq/kg. This activity is slightly above the recommended value for seafood, which is 15 Bq / kg. Based on the calculation, the daily intake for adults is around 0.18-0.41 Bq, for children, it is 0.12-0.27 Bq, and infant are 0.06-0.14 Bq. The daily intake of adults and children exceeds the recommended annual intake limit. However, the annual intake of many countries in the Asian region, including Japan and China, also far exceeds the recommended value. The amount of marine fish consumed significantly influences this annual dose estimate. The estimated dosage of 210Po yearly for adult consumers is 32.3-71.42 µSv/year for children, it ranges from 46.65-103.16 µSv/year, and for infants, it ranges from 78.95-174.58 µSv/year. These values are similar to the UNSCEAR recommended values: around 70, 100, and 180 µSv/year for adults, children, and infants. Ide utama dari penelitian ini adalah untuk menghitung aktivitas 210Po pada biota laut dari perairan Banda Aceh dan menghitung estimasi dosis terhadap konsumen ikan laut. Analisis polonium dilakukan terhadap sampel ikan ikan kaka tua, ikan layur, ikan talang-talang, ikan kawet, dan ikan hiu menggunakan alfa spektrometer. Hasil penelitian menemukan aktivitas 210Po tertinggi pada ikan hiu yaitu 16,90 Bq/kg, dan ikan Kaka tua sebesar 16,24 Bq/kg. Aktivitas ini sedikit diatas nilai yang direkomendasikan untuk makanan laut, yaitu sebesar 15 Bq/kg. Berdasarkan perhitungan, asupan harian terhadap orang dewasa sekitar 0,18–0,41 Bq, untuk anak anak sebesar 0,12–0,27 Bq dan bayi sebesar 0,06-0,14 Bq. Asupan harian dewasa dan anak melebih batas tahunan asupan yang direkomendasikan, namun asupan tahunan dari banyak Negara di kawasan Asia termasuk Jepang dan China juga jauh melebih nilai yang direkomendasikan Jumlah konsumsi ikan laut sangat berpengaruh terhadap estimasi dosis tahunan ini. Penghitungan estimasi dosis 210Po tahunan terhadap konsumen dewasa adalah sebesar 32,3–71,42 µSv/tahun, untuk anak anak berkisar antara 46,65–103,16 µSv/tahun dan untuk bayi berkisar antara 78,95–174,58 µSv/tahun. Nilai ini mirip dengan nilai rekomendasi UNSCEAR, yaitu sekitar 70, 100 dan 180 µSv/tahun untuk dewasa, anak anak dan bayi.

CULTIVATION CONDITIONS FOR PROTEASE PRODUCTION BY A THERMO-HALOSTABLE BACTERIAL ISOLATE PLS A Teuku M. Iqbalsyah; Malahayati Malahayati; Atikah Atikah; Febriani Febriani
Jurnal Natural Volume 19, Number 1, February 2019
Publisher : Universitas Syiah Kuala

Polyextremophiles have increasingly been utilised to produce thermostable enzymes with better stability in multiple extreme conditions. This study reports the screening results of four new bacterial isolates (PLS A, PLS 75, PLS 76 and PLS 80), isolated from an under water hot springs, in producing thermo-halostable protease enzyme. Optimum cultivation conditions for the protease production were also studied. Screening of protease-producing isolates was conducted using Thermus solid medium enriched with 3% skim milk and 0.5% casein. The growth of the isolates showing protease activity was monitored by measuring the cell dry weight and protease activity during 24 h cultivation period. The activity was also measured at various cultivation conditions, i.e. temperature, pH and salt concentrations. Amongst the four isolates, only PLS A showed the ability to produce protease. The optimum cultivation conditions for protease production were observed at 65°C, pH 7 for 18 h incubation. The activity increased with the addition of 1% NaCl concentration (0.085 Unit/mL). The ability of PLS A isolate to produce thermo-halostable protease was encouraging as they could potentially be used in industries requiring the enzyme with multiple extremes.

PHYLOGENETIC ANALYSIS OF 16S rRNA GENE FRAGMENT OF ANTIBIOTIC-PRODUCING PLS 76 ISOLATE Mulia Aria Suzanni; Amelya Yolanda; Nurdin Saidi; Febriani Febriani; Teuku Mohamad Iqbalsyah
Jurnal Natural Volume 18, Number 3, October 2018
Publisher : Universitas Syiah Kuala

Exploitation of extremophiles as novel bioactive compounds sources has been increasing. The aim of this study was to evaluate the activity and the class of antibiotic produced by a thermo-halophilic isolate PLS 76, as well as to identify the genotype of the isolate. The activity was determined by a disc diffusion method, while the antibiotic class was determined qualitatively by chemical reactions using ninhydrin, iodine vapour and potassium iodine. The genotype was determined by sequencing the 16S rRNA gene fragment and the phylogenetic tree from the sequence data. The results showed that PLS 76 was a Gram-negative bacterium and able to produce polypeptide antibiotic, which showed a slight activity on E. coli and S. aureus. Sequence alignment of the 16S rRNA gene fragment showed that PLS 76 was most related to Geobacillus kaustophilus. These results may be used to utilise the isolated for further antibiotic study.

Exploration study of indigenous civet (Paradoxorus hermaphroditus) bacteria: isolates characterization and molecular identification Murna Muzaifa; Yusya Abubakar; Febriani Febriani; Amhar Abubakar
AGROINTEK Vol 17, No 1 (2023)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v17i1.13841

Biochemistry of civet coffee fermentation in the digestive tract of civets until now is not fully known. This study aims to isolation, characterization and identification of bacteria molecularly from the civet's digestive tract. Analysis of bacteria begins with the civet surgery to obtain fluid from three parts of the civet's digestive tract. Isolation of bacteria conducted by pour plate method using nutrient agar media. Single and different colonies (shape, colour, margin, elevation, size) were separated and purified by streaking method. The pure isolate was further characterized biochemically. The biochemical characteristics of the bacteria observed were Gram staining, catalase, protease and pectinase activity. Identification of the bacteria was carried out molecularly by identifying the 16S rRNA gene. The results of molecular identification showed that the bacteria from the civet were identified as Alcaligenes faecalis strain LHW 1749, Ochrobactrum anthropi, Stenotrophomonas sp. 262 and Bacillus sp.

Calcined Aceh Bovine Bone (Bos indicus) Intercalated Lithium as An Inorganic Base Catalyst for Transesterification of Castor Oil Muliadi Ramli; Saiful Saiful; Febriani Febriani; Amraini Amraini; Fathurrahmi Fathurrahmi; Shellatina Shellatina; Cut Fatimah Zuhra
Aceh International Journal of Science and Technology Vol 9, No 1 (2020): April 2020
Publisher : Graduate Program of Syiah Kuala University

Realizing abundant availability of local bovine bone wastes in Aceh Province, Indonesia, this study is subjected to take competitive advantages from the local bovine bone waste for preparing an inorganic catalyst preparation. In detail, calcined Aceh bovine bones were successfully intercalated lithium through an impregnation method resulting in the inorganic base catalyst, which showed promising activity in the transesterification of castor oil. Prior to the experiment, the Aceh bovine bone waste was calcined at 900 oC in the air atmosphere for 4 hours, led to forming crystalline phases of hydroxyapatite [Ca5(PO4)3(OH)] and calcium oxide [CaO]. Also, the chemical modification of calcined bovine bone with lithium precursor has enhanced the physicochemical properties of the inorganic base catalyst. Finally, the intercalated Aceh bovine bone has shown a feasible activity in transesterification of castor oil into biodiesel, which yielding methyl ricinoleate as the main product.

Isolation and characterization of polyhydroxyalkanoate (PHA) producing bacteria isolate from landfill land of Kampung Jawa Banda Aceh MAZWAN MAZWAN; FEBRIANI FEBRIANI; NAZARUDDIN NAZARUDDIN
Jurnal Natural Volume 23 Number 1, February 2023
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v23i1.29746

The production of biodegradable plastic from microorganisms has great potential as a substitute for conventional plastic. This study aims to isolate bacterial strains capable of polyhydroxyalkanoates (PHAs) production from the Kampung Jawa landfill land (KJLL) and characterize biopolymers. The bacterial strains were able to produce PHA using a mineral salt medium (MSM) with glucose as a carbon source. The qualitative screening of PHA-producing bacteria was conducted by Sudan Black and Nile Red. Of the 64 bacteria strains, only 41 were able to accumulate PHA in Sudan Black and Nile Red. The results showed that one bacteria the Coccobacillus strain had the highest color intensity for further characterization of PHA. The characterization of PHA by Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) showed a melting temperature (Tm) of 101.54°C. X-ray diffraction (XRD) analysis revealed a crystalline structure with an index crystallinity (ICr) value of 15.82% for PHA. The results of the analysis proved that PHA was produced by bacteria isolate. This study suggests that this is the first report of the bacteria from the Kampung Jawa landfill producing PHA with good characteristics and potential biotechnology applications.

PHYLOGENETIC ANALYSIS OF 16S rRNA GENE FRAGMENT OF ANTIBIOTIC-PRODUCING PLS 76 ISOLATE Mulia Aria Suzanni; Amelya Yolanda; Nurdin Saidi; Febriani Febriani; Teuku Mohamad Iqbalsyah
Jurnal Natural Volume 18, Number 3, October 2018
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v18i3.11152

Exploitation of extremophiles as novel bioactive compounds sources has been increasing. The aim of this study was to evaluate the activity and the class of antibiotic produced by a thermo-halophilic isolate PLS 76, as well as to identify the genotype of the isolate. The activity was determined by a disc diffusion method, while the antibiotic class was determined qualitatively by chemical reactions using ninhydrin, iodine vapour and potassium iodine. The genotype was determined by sequencing the 16S rRNA gene fragment and the phylogenetic tree from the sequence data. The results showed that PLS 76 was a Gram-negative bacterium and able to produce polypeptide antibiotic, which showed a slight activity on E. coli and S. aureus. Sequence alignment of the 16S rRNA gene fragment showed that PLS 76 was most related to Geobacillus kaustophilus. These results may be used to utilise the isolated for further antibiotic study.

Isolation and characterization of polyhydroxyalkanoate (PHA) producing bacteria isolate from landfill land of Kampung Jawa Banda Aceh MAZWAN MAZWAN; FEBRIANI FEBRIANI; NAZARUDDIN NAZARUDDIN
Jurnal Natural Volume 23 Number 1, February 2023
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v23i1.29746

The production of biodegradable plastic from microorganisms has great potential as a substitute for conventional plastic. This study aims to isolate bacterial strains capable of polyhydroxyalkanoates (PHAs) production from the Kampung Jawa landfill land (KJLL) and characterize biopolymers. The bacterial strains were able to produce PHA using a mineral salt medium (MSM) with glucose as a carbon source. The qualitative screening of PHA-producing bacteria was conducted by Sudan Black and Nile Red. Of the 64 bacteria strains, only 41 were able to accumulate PHA in Sudan Black and Nile Red. The results showed that one bacteria the Coccobacillus strain had the highest color intensity for further characterization of PHA. The characterization of PHA by Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) showed a melting temperature (Tm) of 101.54°C. X-ray diffraction (XRD) analysis revealed a crystalline structure with an index crystallinity (ICr) value of 15.82% for PHA. The results of the analysis proved that PHA was produced by bacteria isolate. This study suggests that this is the first report of the bacteria from the Kampung Jawa landfill producing PHA with good characteristics and potential biotechnology applications.

Title

Found 10 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 10 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 10 Documents
Search