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All Journal Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture
B. TJ. Hariadi
Faculty of Animal Science, University of Papua, Jl. Gunung Salju, Amban, Manokwari, Papua 98314
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Veterinary

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Effect of level of lactic acid bacteria inoculant from fermented grass extract on fermentation quality of king grass silage Antaribaba, M.A; Tero, N.K; Hariadi, B. TJ.; Santoso, B.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 4 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (69.454 KB) | DOI: 10.14334/jitv.v14i4.316

Abstract

Ensiling is a method of preserving moist forage based on natural fermentation where lactic acid bacteria (LAB) ferment water soluble carbohydrate into organic acids mainly lactic acid under anaerobic condition. The aim of this study was to evaluate the quality of king grass (Pennisetum purpureophoides) ensiled with addition of LAB prepared from fermented grass extract (LBFG). Four treatment were (G0) king grass without additive; (G1) king grass with 2% (v/w) of LBFG; (G2) king grass with 3% (v/w) of LBFG; (G3) king grass with 4% (v/w) of LBFG. Ensiling was conducted in bottle silos of 225 g capacity at room temperatures (27.0 ± 0.20C) for 30 days. The results showed that crude protein content in silage G1, G2 and G3 were relatively higher than that in silage G0. The pH value, butyric acid, total VFA and NH3-N concentrations decreased linearly with increasing level of LBFG addition, while lactic acid concentration increased linearly with LBFG addition. It was concluded that addition of 3% (v/w) of LBFG resulting a better fermentation quality of king grass silage than 2% and 4% (v/w) of LBFG. Key words: Silage, Lactic Acid, Fermentation, King Grass
THE EFFECT OF CONCENTRATE CONTAINING PROBIOTICS ON FERMENTATION CHARACTERISTICS, METHANOGENESIS AND In vitro NUTRIENT DIGESTILITY Santoso, B.; Hariadi, B. Tj.; Abubakar, H.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 4 (2014): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.4.210-216

Abstract

The aim of the experiment was to evaluate the effect of probiotic addition in concentrate onfermentation characteristics, methane (CH4) production and in vitro nutrient digestibility. Two strainslactic acid bacteria (LAB) i.e. Lactobacillus plantarum and Lactobacillus acidhophilus, and one strainyeast of Saccharomyces cerevisiae was used as probiotic.This experiment was arranged in a completelyrandomized design consisted of 4 treatments as follows grass silage (G); grass silage + concentratewithout probiotic (G+A); grass silage + concentrate containing L. plantarum and. S. cerevisiae (G+B);grass silage + concentrate containing L. acidophilus and. S. cerevisiae (G+C); grass silage + concentratecontaining L. plantarum and L. acidophilus (G+D). Data were analyzed as completely randomizeddesign and followed by Duncan’s multiple range test. The results showed that the concentrate containingLAB varied 1.5 × 106 and 3 × 107 cfu/g, and 3 × 103 cfu/g of S. cerevisiae. Combination between L.plantarum and S. cerevisiae (G+B), and L. acidophilus and S. cerevisiae (G+C) in concentrate increased(P<0.01) propionic acid proportion. The average CH4 production in concentrate containing probiotic(G+C, G+D and D+E) was lower by 6.9% (P<0.01) compared to concentrate without probiotic (G+B). The in vitro dry matter (DM) and neutral detergent fiber (NDF) digestibility were higher (P<0.01) by25.7% and 6.3% respectively, in grass silage substrate with concentrate containing probiotic (G+C, G+Dand G+E) than in grass silage with concentrate without probiotic (G+B). In conclusion, addition ofprobiotic in concentrate could increase in vitro propionic acid proportion, DM and NDF digestibility andreduce CH4 production.
FERMENTATION QUALITY OF KING GRASS SILAGE TREATED WITH LIQUID OR DRIED INOCULANT OF LACTIC ACID BACTERIA Santoso, B.; Hariadi, B. Tj.; Jeni, J.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 4 (2015): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.4.208-214

Abstract

The aim of this study was to evaluate the nutritive value and fermentation characteristic of king grass silage treated with addition of liquid or dried lactic acid bacteria (LAB) inoculant. Experiment was arranged to a completely randomized design with four treatments and three replications. Four treatments as follows (A) king grass without LAB inoculant as the control; (B) king grass + 30 ml of liquid of epiphytic LAB inoculant/kg of fresh forage; (C) king grass + 30 g of freeze-dried powder of LAB inoculant/kg of fresh forage; (D) king grass + 30 g of centrifuged powder of LAB inoculant/kg of fresh forage. The liquid LAB inoculant was sprayed on the top of grass and then mannualy mixed by hand. The intial LAB concentration in all inoculants were 1.0 × 106 cfu/g. About 500 g of silage materials were ensiled into plastic silos and stored at room temperatures (approximately 28oC) for 30 days. Results showed that pH value, concentrations of lactic acid, N-amonia (NH3-N), butyric acid and total volatile fatty acids (VFA) as well as Fleigh Point were affected by treatment of LAB inoculant. Silage treated with liquid or dried of LAB inoculant had lower (P
Co-Authors B. Santoso H. Abubakar, H. Jeni Jeni M.A Antaribaba N.K Tero