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All Journal Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
Agus Nuryanto
Faculty of Biology Jenderal Soedirman University
Earth & Planetary Sciences Education Engineering

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Resistensi dan karakter molekuler benih gurami sowang Osphronemus goramy Lacepede, 1801 asal induk berbeda Kusbiyanto Kusbiyanto; Agus Nuryanto; Petrus H. T. Soedibja
Depik Vol 6, No 3 (2017): December 2017
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (489.309 KB) | DOI: 10.13170/depik.6.3.6539

Abstract

The cultivation of giant gourami is constrained by its high mortality due to low resistance to diseases. Resistance is an inherited character from the parental to their seeds. High resistance seeds can be selected using molecular marker, such as Major Histocompatibility complex (MHC) gene. Resistance character is assumed to be different among individual from different broodstocks and is suggested related to their genetic constituent.  This research aims to analyze the resistance of sowang gouramy seeds from different broodstocks and describe genetic character of seeds from different broodstocks. An explorative survey was perfomed. One hundred individuals were taken purposively from Balai Benih Ikan Sikamaju Ciamis West Java and hundred individuals were bought from fish farmer in Ciamis West Java. Sampling was performed in 2015 for the first seed group and in 2016 for the second seed group. Samples were subjected to Aeromonas hydrophila and Pseudomonas aeruginosa infection. The fragments of MHC gene were amplified using PCR technique from eight individual of first group and six individuals of second group. The resistance characteristic was analyzed using simple mathematics based on the number of living seeds compared total infected seeds and molecular characteristics was analyzed descriptively based on DNA band pattern. Different resistance to A. hydrophila was observed between seed groups from different broodstocks. The seeds group from the first broodstocks showed lower resistance level with the value of 29% than that from the second broodstocks with the resistance value reached of 100%. Both seed groups also showed resistance differences to P.aeruginosa, although  not as high as A.hydrophila infection. The differencies were also reflected in their MHC gene between seed groups from two different broodstocks. The amplication of MHC gene of the seeds from the first broodstacks resulted of 585 bp and 400 bp length fragments, while from those second broodstocks was only resulted of 400 bp frament. The differences on DNA band pattern between seed groups indicate a different molecular characteristics among seeds from different broodstocks.Usaha budidaya ikan gurami terhambat oleh tingginya mortalitas benih karena rendahnya resistensi terhadap penyakit. Resistensi merupakan sifat yang diwariskan dari tetua ke anakan. Benih yang resisten dapat diseleksimenggunakan marka molekuler seperti gen Major Histocompatibility complex (MHC). Sifat resisten diduga berbeda diantara benih yang beraasal dari induk berbeda dan diduga terkait dengan kompenen genetik yang dimiliki. Tujuan penelitian ini adalah untuk mengetahui sifat reisten dan karakter molekuler benih gurami sowang asal induk berbeda. Fragmen gen MHC diamplifikasimenggunakan teknik PCR. Sifat resistensi dianalisis menggunakan perhitungan matematika sederhana berdasarkan jumlah benih hidup dibagi jumlah total benih yang diinfeksi, sedangkan karakter molekuler dianalisis secara deskritif berdasakan pola pita yang dihasilkan. Kedua kelompok benih memiliki sifat resistensi berbeda terhadap Aeromonas hydrophila. Benih dari induk pertama memiliki sifat resistensi lebih rendah dengan nilai kelangsungan hidup sebesar 29% daripada kelompok benih dari induk kedua yang memiliki kelangsungan hidup mencapai 100%. Kedua kelompok benih juga memperlihatkan perbedaan sifat resisten terhadap P. aeruginosa, meskipun perbedaannya tidak sebesar terhadap A.hydrophila. Perbedaan tersebut juga tercermin pada gen MHC kedua kelompok benih. Pada kelompok benih pertama dihasilkan dua fragmen gen MHC dengan ukuran 585 pb dan 400 pb, sedangkan dari kelompok benih kedua hanya dihasilkan fragmen berukeuran 400 pb. Perbedaan pola pita diantara kedua kelompok benih tersebut merupakan idikasi adanya perbedaan karakter molekuler pada benih yang berasal dari dua induk berbeda
Phylogenetic analysis of ornamental Pomacentridae from the southern coast of West Java Munatul Khayah Almusyarofah; Romanus Edy Prabowo; Agus Nuryanto
Depik Vol 9, No 1 (2020): April 2020
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (622.109 KB) | DOI: 10.13170/depik.9.1.14722

Abstract

Abstract. Pomacentridae has a wide distribution in Indonesia waters. Research on species richness of Pomacentridae has been carried out in some areas in Indonesia. However, there are no data about the relationships of Pomacentridae on the southern coast of West Java. This research aims to know the evolutionary kinship of Pomacentridae ornamental fish in Ujung Genteng and Taman Manalusu Beach, West Java. This research used a survey method with a judgment sampling technique. The parameters measured were the consistency index (CI) and character evolution of the resulting phylogenetic tree. Cladogram reconstructed based on maximum parsimony algorithm with 1000 bootstrap replicates in PAUP software. The result showed that the tree had a CI value of 0.6000 which indicated low homoplasy. Pomacentridae formed a monophyletic group when they compared with Chaetodon collare as outgroup. The Pomacentridae clade divided into two subclades. The first subclade consisted of Abudefduf indicus as a basal species, then Neoglypydodon bonang and Plectroglyphidodon lacrymatus have a closed kinship and diverged after Dascyllus trimaculatus, and then Abudefduf notatus and Abudefduf sexfaciatus have a close kinship and diverged after Abudefduf vaigiensis. The second subclade referred to as a basal group, and within this subclade, Chrysiptera brownriggii has a closely related to Chrysiptera unimaculata and diverged after Chromis opercularis.Keywords: Cladistics, damselfish, meristic, morphometric, Pomacentridae
Phylogenetic analysis of ornamental Pomacentridae from the southern coast of West Java Munatul Khayah Almusyarofah; Romanus Edy Prabowo; Agus Nuryanto
Depik Vol 9, No 1 (2020): April 2020
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.9.1.14722

Abstract

Abstract. Pomacentridae has a wide distribution in Indonesia waters. Research on species richness of Pomacentridae has been carried out in some areas in Indonesia. However, there are no data about the relationships of Pomacentridae on the southern coast of West Java. This research aims to know the evolutionary kinship of Pomacentridae ornamental fish in Ujung Genteng and Taman Manalusu Beach, West Java. This research used a survey method with a judgment sampling technique. The parameters measured were the consistency index (CI) and character evolution of the resulting phylogenetic tree. Cladogram reconstructed based on maximum parsimony algorithm with 1000 bootstrap replicates in PAUP software. The result showed that the tree had a CI value of 0.6000 which indicated low homoplasy. Pomacentridae formed a monophyletic group when they compared with Chaetodon collare as outgroup. The Pomacentridae clade divided into two subclades. The first subclade consisted of Abudefduf indicus as a basal species, then Neoglypydodon bonang and Plectroglyphidodon lacrymatus have a closed kinship and diverged after Dascyllus trimaculatus, and then Abudefduf notatus and Abudefduf sexfaciatus have a close kinship and diverged after Abudefduf vaigiensis. The second subclade referred to as a basal group, and within this subclade, Chrysiptera brownriggii has a closely related to Chrysiptera unimaculata and diverged after Chromis opercularis.Keywords: Cladistics, damselfish, meristic, morphometric, Pomacentridae
Resistensi dan karakter molekuler benih gurami sowang Osphronemus goramy Lacepede, 1801 asal induk berbeda Kusbiyanto Kusbiyanto; Agus Nuryanto; Petrus H. T. Soedibja
Depik Vol 6, No 3 (2017): December 2017
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.6.3.6539

Abstract

The cultivation of giant gourami is constrained by its high mortality due to low resistance to diseases. Resistance is an inherited character from the parental to their seeds. High resistance seeds can be selected using molecular marker, such as Major Histocompatibility complex (MHC) gene. Resistance character is assumed to be different among individual from different broodstocks and is suggested related to their genetic constituent.  This research aims to analyze the resistance of sowang gouramy seeds from different broodstocks and describe genetic character of seeds from different broodstocks. An explorative survey was perfomed. One hundred individuals were taken purposively from Balai Benih Ikan Sikamaju Ciamis West Java and hundred individuals were bought from fish farmer in Ciamis West Java. Sampling was performed in 2015 for the first seed group and in 2016 for the second seed group. Samples were subjected to Aeromonas hydrophila and Pseudomonas aeruginosa infection. The fragments of MHC gene were amplified using PCR technique from eight individual of first group and six individuals of second group. The resistance characteristic was analyzed using simple mathematics based on the number of living seeds compared total infected seeds and molecular characteristics was analyzed descriptively based on DNA band pattern. Different resistance to A. hydrophila was observed between seed groups from different broodstocks. The seeds group from the first broodstocks showed lower resistance level with the value of 29% than that from the second broodstocks with the resistance value reached of 100%. Both seed groups also showed resistance differences to P.aeruginosa, although  not as high as A.hydrophila infection. The differencies were also reflected in their MHC gene between seed groups from two different broodstocks. The amplication of MHC gene of the seeds from the first broodstacks resulted of 585 bp and 400 bp length fragments, while from those second broodstocks was only resulted of 400 bp frament. The differences on DNA band pattern between seed groups indicate a different molecular characteristics among seeds from different broodstocks.Usaha budidaya ikan gurami terhambat oleh tingginya mortalitas benih karena rendahnya resistensi terhadap penyakit. Resistensi merupakan sifat yang diwariskan dari tetua ke anakan. Benih yang resisten dapat diseleksimenggunakan marka molekuler seperti gen Major Histocompatibility complex (MHC). Sifat resisten diduga berbeda diantara benih yang beraasal dari induk berbeda dan diduga terkait dengan kompenen genetik yang dimiliki. Tujuan penelitian ini adalah untuk mengetahui sifat reisten dan karakter molekuler benih gurami sowang asal induk berbeda. Fragmen gen MHC diamplifikasimenggunakan teknik PCR. Sifat resistensi dianalisis menggunakan perhitungan matematika sederhana berdasarkan jumlah benih hidup dibagi jumlah total benih yang diinfeksi, sedangkan karakter molekuler dianalisis secara deskritif berdasakan pola pita yang dihasilkan. Kedua kelompok benih memiliki sifat resistensi berbeda terhadap Aeromonas hydrophila. Benih dari induk pertama memiliki sifat resistensi lebih rendah dengan nilai kelangsungan hidup sebesar 29% daripada kelompok benih dari induk kedua yang memiliki kelangsungan hidup mencapai 100%. Kedua kelompok benih juga memperlihatkan perbedaan sifat resisten terhadap P. aeruginosa, meskipun perbedaannya tidak sebesar terhadap A.hydrophila. Perbedaan tersebut juga tercermin pada gen MHC kedua kelompok benih. Pada kelompok benih pertama dihasilkan dua fragmen gen MHC dengan ukuran 585 pb dan 400 pb, sedangkan dari kelompok benih kedua hanya dihasilkan fragmen berukeuran 400 pb. Perbedaan pola pita diantara kedua kelompok benih tersebut merupakan idikasi adanya perbedaan karakter molekuler pada benih yang berasal dari dua induk berbeda
Phylogenetic analysis of ornamental Pomacentridae from the southern coast of West Java Munatul Khayah Almusyarofah; Romanus Edy Prabowo; Agus Nuryanto
Depik Vol 9, No 1 (2020): April 2020
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.9.1.14722

Abstract

Abstract. Pomacentridae has a wide distribution in Indonesia waters. Research on species richness of Pomacentridae has been carried out in some areas in Indonesia. However, there are no data about the relationships of Pomacentridae on the southern coast of West Java. This research aims to know the evolutionary kinship of Pomacentridae ornamental fish in Ujung Genteng and Taman Manalusu Beach, West Java. This research used a survey method with a judgment sampling technique. The parameters measured were the consistency index (CI) and character evolution of the resulting phylogenetic tree. Cladogram reconstructed based on maximum parsimony algorithm with 1000 bootstrap replicates in PAUP software. The result showed that the tree had a CI value of 0.6000 which indicated low homoplasy. Pomacentridae formed a monophyletic group when they compared with Chaetodon collare as outgroup. The Pomacentridae clade divided into two subclades. The first subclade consisted of Abudefduf indicus as a basal species, then Neoglypydodon bonang and Plectroglyphidodon lacrymatus have a closed kinship and diverged after Dascyllus trimaculatus, and then Abudefduf notatus and Abudefduf sexfaciatus have a close kinship and diverged after Abudefduf vaigiensis. The second subclade referred to as a basal group, and within this subclade, Chrysiptera brownriggii has a closely related to Chrysiptera unimaculata and diverged after Chromis opercularis.Keywords: Cladistics, damselfish, meristic, morphometric, Pomacentridae
Resistensi dan karakter molekuler benih gurami sowang Osphronemus goramy Lacepede, 1801 asal induk berbeda Kusbiyanto Kusbiyanto; Agus Nuryanto; Petrus H. T. Soedibja
Depik Vol 6, No 3 (2017): December 2017
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.6.3.6539

Abstract

The cultivation of giant gourami is constrained by its high mortality due to low resistance to diseases. Resistance is an inherited character from the parental to their seeds. High resistance seeds can be selected using molecular marker, such as Major Histocompatibility complex (MHC) gene. Resistance character is assumed to be different among individual from different broodstocks and is suggested related to their genetic constituent.  This research aims to analyze the resistance of sowang gouramy seeds from different broodstocks and describe genetic character of seeds from different broodstocks. An explorative survey was perfomed. One hundred individuals were taken purposively from Balai Benih Ikan Sikamaju Ciamis West Java and hundred individuals were bought from fish farmer in Ciamis West Java. Sampling was performed in 2015 for the first seed group and in 2016 for the second seed group. Samples were subjected to Aeromonas hydrophila and Pseudomonas aeruginosa infection. The fragments of MHC gene were amplified using PCR technique from eight individual of first group and six individuals of second group. The resistance characteristic was analyzed using simple mathematics based on the number of living seeds compared total infected seeds and molecular characteristics was analyzed descriptively based on DNA band pattern. Different resistance to A. hydrophila was observed between seed groups from different broodstocks. The seeds group from the first broodstocks showed lower resistance level with the value of 29% than that from the second broodstocks with the resistance value reached of 100%. Both seed groups also showed resistance differences to P.aeruginosa, although  not as high as A.hydrophila infection. The differencies were also reflected in their MHC gene between seed groups from two different broodstocks. The amplication of MHC gene of the seeds from the first broodstacks resulted of 585 bp and 400 bp length fragments, while from those second broodstocks was only resulted of 400 bp frament. The differences on DNA band pattern between seed groups indicate a different molecular characteristics among seeds from different broodstocks.Usaha budidaya ikan gurami terhambat oleh tingginya mortalitas benih karena rendahnya resistensi terhadap penyakit. Resistensi merupakan sifat yang diwariskan dari tetua ke anakan. Benih yang resisten dapat diseleksimenggunakan marka molekuler seperti gen Major Histocompatibility complex (MHC). Sifat resisten diduga berbeda diantara benih yang beraasal dari induk berbeda dan diduga terkait dengan kompenen genetik yang dimiliki. Tujuan penelitian ini adalah untuk mengetahui sifat reisten dan karakter molekuler benih gurami sowang asal induk berbeda. Fragmen gen MHC diamplifikasimenggunakan teknik PCR. Sifat resistensi dianalisis menggunakan perhitungan matematika sederhana berdasarkan jumlah benih hidup dibagi jumlah total benih yang diinfeksi, sedangkan karakter molekuler dianalisis secara deskritif berdasakan pola pita yang dihasilkan. Kedua kelompok benih memiliki sifat resistensi berbeda terhadap Aeromonas hydrophila. Benih dari induk pertama memiliki sifat resistensi lebih rendah dengan nilai kelangsungan hidup sebesar 29% daripada kelompok benih dari induk kedua yang memiliki kelangsungan hidup mencapai 100%. Kedua kelompok benih juga memperlihatkan perbedaan sifat resisten terhadap P. aeruginosa, meskipun perbedaannya tidak sebesar terhadap A.hydrophila. Perbedaan tersebut juga tercermin pada gen MHC kedua kelompok benih. Pada kelompok benih pertama dihasilkan dua fragmen gen MHC dengan ukuran 585 pb dan 400 pb, sedangkan dari kelompok benih kedua hanya dihasilkan fragmen berukeuran 400 pb. Perbedaan pola pita diantara kedua kelompok benih tersebut merupakan idikasi adanya perbedaan karakter molekuler pada benih yang berasal dari dua induk berbeda
Co-Authors Kusbiyanto Kusbiyanto Munatul Khayah Almusyarofah Petrus H. T. Soedibja Romanus Edy Prabowo