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All Journal Biospecies Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
Tarsim Tarsim
Universitas Lampung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Engineering Immunology & microbiology

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Efektivitas Pemberian Bacillus sp. D2.2 pada Media Teknis Molase terhadap Kualitas Air dan Performa Pertumbuhan Udang Vaname (Litopenaeus vannamei): The Effectivity of Bacillus sp. D2.2 in Molasses Technical Medium to Water Quality and Growth Performance of Vaname Prawns (Litopenaeus vannamei) Ayu NOVITASARI; Ricky Nur ISKANDAR; Hefi Afizena ELVAZIA; Esti HARPENI; Tarsim TARSIM; Wardiyanto WARDIYANTO
Biospecies Vol. 10 No. 2 (2017): Juli 2017
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1411.08 KB) | DOI: 10.22437/biospecies.v10i2.3824

Abstract

Vanamei prawns have fast growth and can reared in high density. it has an impact on water quality deterioration and disruption of survival rates and growth. Various ways to cope has been done, one of them is with probiotic bacteria. The new strain of D2.2 bacteria is thought to be effective of ammonia utilization. Probiotics with local bacteria Bacillus sp. D2.2 is cultured on molasses technical medium to be applied semi-mass. The purpose of this study is to asses the efectivity of Bacillus sp. D2.2 inthe molasses technical medium on water quality and growth performance of vaname prawns (Litopenaeus vannamei). The research was used complete randomized design (RAL) with four treatments, A (Control), B (Application of 5 ppm Bacillus sp. D2.2 cultured in molasses technical medium), C (Application of 10 ppm Bacillus sp. D2.2 cultured in molasses technical medium), D (Application of 15 ppm Bacillus sp. D2.2 cultured in molasses technical medium) were repeated three times each. The results showed no effect on water quality and shrimp survival rate, but absolute growth (W), daily growth rate (GR) and feed conversion ratio (FCR) showed that B and C treatment had better than control. Keywords: Vaname shrimp, growth, Bacillus sp. D2.2, molasses technical medium
Potensi sinbiotik lokal terhadap respon imun non spesifik udang vaname (Litopenaeus vannamei) Indri Saputri Ramadhani; Esti Harpeni; Tarsim Tarsim; Limin Santoso
Depik Vol 6, No 3 (2017): December 2017
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.995 KB) | DOI: 10.13170/depik.6.3.7613

Abstract

The aims of the study were to examine the exact percentage of prebiotics in sinbiotics, administered through feed, so as to enhance nonspecific immune responses in white shrimp. The sweet potato extract was used as prebiotic, combined with local isolated Bacillus sp. D2.2 as a probiotic applied simultaneously as a synbiotic. The feed was treated with 0% sinbiotic treatment (treatment A / control), 0% prebiotic and 6% probiotics (B treatment), 2% prebiotic and 6% probiotics (treatment C), 4% prebiotics and 6% probiotics (treatment D). Examination of nonspecific immune responses to shrimp included total hemocyte count (THC), phagocytosis activity (AP), phagocytosis index (IP), phenoloxidase activity (PO), and superoxide dismutase activity (SOD). Observation of the non-specific immune response of vaname shrimp after treatment showed that treatment C was the best prebiotic  to increase non-specific immune response in vaname shrimp.Penelitian ini bertujuan untuk mempelajari persentase prebiotik yang tepat dalam sinbiotik, yang diberikan melalui pakan, sehingga dapat meningkatkan respon imun nonspesifik pada udang vaname. Dalam penelitian ini digunakan ekstrak tepung ubi jalar sebagai prebiotik, dikombinasikan dengan isolat lokal Bacillus sp. D2.2 sebagai probiotik yang diaplikasikan secara bersamaan sebagai sinbiotik. Pakan yang diberikan adalah pakan dengan perlakuan 0% sinbiotik (perlakuan A/kontrol), 0% prebiotik dan 6% probiotik (perlakuan B), 2% prebiotik dan 6% probiotik (perlakuan C), 4% prebiotik dan 6% probiotik (perlakuan D). Pemeriksaan respon imun nonspesifik pada udang meliputi total hemocyte count (THC), aktivitas fagositosis (AF), indeks fagositosis (IF), aktivitas phenoloxidase (PO), dan aktivitas superoxide dismutase (SOD). Pengamatan pada respon imun non spesifik udang vaname setelah diberi perlakuan menunjukan bahwa perlakuan C merupakan persentase prebiotik terbaik untuk meningkatkan respon imun non spesifik pada udang vaname.
Potensi sinbiotik lokal terhadap respon imun non spesifik udang vaname (Litopenaeus vannamei) Indri Saputri Ramadhani; Esti Harpeni; Tarsim Tarsim; Limin Santoso
Depik Vol 6, No 3 (2017): December 2017
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.6.3.7613

Abstract

The aims of the study were to examine the exact percentage of prebiotics in sinbiotics, administered through feed, so as to enhance nonspecific immune responses in white shrimp. The sweet potato extract was used as prebiotic, combined with local isolated Bacillus sp. D2.2 as a probiotic applied simultaneously as a synbiotic. The feed was treated with 0% sinbiotic treatment (treatment A / control), 0% prebiotic and 6% probiotics (B treatment), 2% prebiotic and 6% probiotics (treatment C), 4% prebiotics and 6% probiotics (treatment D). Examination of nonspecific immune responses to shrimp included total hemocyte count (THC), phagocytosis activity (AP), phagocytosis index (IP), phenoloxidase activity (PO), and superoxide dismutase activity (SOD). Observation of the non-specific immune response of vaname shrimp after treatment showed that treatment C was the best prebiotic  to increase non-specific immune response in vaname shrimp.Penelitian ini bertujuan untuk mempelajari persentase prebiotik yang tepat dalam sinbiotik, yang diberikan melalui pakan, sehingga dapat meningkatkan respon imun nonspesifik pada udang vaname. Dalam penelitian ini digunakan ekstrak tepung ubi jalar sebagai prebiotik, dikombinasikan dengan isolat lokal Bacillus sp. D2.2 sebagai probiotik yang diaplikasikan secara bersamaan sebagai sinbiotik. Pakan yang diberikan adalah pakan dengan perlakuan 0% sinbiotik (perlakuan A/kontrol), 0% prebiotik dan 6% probiotik (perlakuan B), 2% prebiotik dan 6% probiotik (perlakuan C), 4% prebiotik dan 6% probiotik (perlakuan D). Pemeriksaan respon imun nonspesifik pada udang meliputi total hemocyte count (THC), aktivitas fagositosis (AF), indeks fagositosis (IF), aktivitas phenoloxidase (PO), dan aktivitas superoxide dismutase (SOD). Pengamatan pada respon imun non spesifik udang vaname setelah diberi perlakuan menunjukan bahwa perlakuan C merupakan persentase prebiotik terbaik untuk meningkatkan respon imun non spesifik pada udang vaname.
Potensi sinbiotik lokal terhadap respon imun non spesifik udang vaname (Litopenaeus vannamei) Indri Saputri Ramadhani; Esti Harpeni; Tarsim Tarsim; Limin Santoso
Depik Vol 6, No 3 (2017): December 2017
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.6.3.7613

Abstract

The aims of the study were to examine the exact percentage of prebiotics in sinbiotics, administered through feed, so as to enhance nonspecific immune responses in white shrimp. The sweet potato extract was used as prebiotic, combined with local isolated Bacillus sp. D2.2 as a probiotic applied simultaneously as a synbiotic. The feed was treated with 0% sinbiotic treatment (treatment A / control), 0% prebiotic and 6% probiotics (B treatment), 2% prebiotic and 6% probiotics (treatment C), 4% prebiotics and 6% probiotics (treatment D). Examination of nonspecific immune responses to shrimp included total hemocyte count (THC), phagocytosis activity (AP), phagocytosis index (IP), phenoloxidase activity (PO), and superoxide dismutase activity (SOD). Observation of the non-specific immune response of vaname shrimp after treatment showed that treatment C was the best prebiotic  to increase non-specific immune response in vaname shrimp.Penelitian ini bertujuan untuk mempelajari persentase prebiotik yang tepat dalam sinbiotik, yang diberikan melalui pakan, sehingga dapat meningkatkan respon imun nonspesifik pada udang vaname. Dalam penelitian ini digunakan ekstrak tepung ubi jalar sebagai prebiotik, dikombinasikan dengan isolat lokal Bacillus sp. D2.2 sebagai probiotik yang diaplikasikan secara bersamaan sebagai sinbiotik. Pakan yang diberikan adalah pakan dengan perlakuan 0% sinbiotik (perlakuan A/kontrol), 0% prebiotik dan 6% probiotik (perlakuan B), 2% prebiotik dan 6% probiotik (perlakuan C), 4% prebiotik dan 6% probiotik (perlakuan D). Pemeriksaan respon imun nonspesifik pada udang meliputi total hemocyte count (THC), aktivitas fagositosis (AF), indeks fagositosis (IF), aktivitas phenoloxidase (PO), dan aktivitas superoxide dismutase (SOD). Pengamatan pada respon imun non spesifik udang vaname setelah diberi perlakuan menunjukan bahwa perlakuan C merupakan persentase prebiotik terbaik untuk meningkatkan respon imun non spesifik pada udang vaname.
Co-Authors Ayu NOVITASARI Esti Harpeni Hefi Afizena ELVAZIA Indri Saputri Ramadhani Limin Santoso Ricky Nur ISKANDAR Wardiyanto WARDIYANTO