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All Journal Biotropika Indonesian Journal of Human Nutrition
Sakinah Hilya Abida
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Efek Refugia Tanaman Jagung (Zea mays) dan Tanaman Kacang Panjang (Vigna cylindrica) pada Pola Kunjungan Serangga di Sawah Padi (Oryza sativa) Dusun Balong, Karanglo, Malang Yuris Setyadin; Sakinah Hilya Abida; Haidar Azzamuddin; S. Fatiyatur Rahmah; Amin Setyo Leksono
Biotropika: Journal of Tropical Biology Vol 5, No 2 (2017)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2017.005.02.3

Abstract

Hama padi merupakan masalah yang menuntut untuk ditangani secara efektif, mudah, dan murah. Penggunaan pestisida sebagai solusi terbukti membawa dampak negatif sehingga perlu dikembangkan inovasi pengendalian hama, salah satunya adalah refugia. Refugia jagung dan kacang panjang menjadi salah satu solusi terbaik dalam berbagai aspek. Penelitian ini bertujuan untuk mengetahui kelimpahan, struktur komunitas, dan diversitas serangga di lahan persawahan padi di Desa Tunjungtirto, Kecamatan Singosari, Kabupaten Malang. Metode yang digunakan adalah visual kontrol terhadap serangga pengunjung blog refugia. Pengambilan data dilakukan sebanyak tiga kali sehari pada fase vegetatif dan generatif tanaman refugia selama 15 menit. Hasil penelitian menunjukkan bahwa tanaman refugia di sawah padi dapat menyeimbangkan populasi serangga herbivora, predator, dan polinator. Dibuktikan dengan persentase kelimpahan relatif herbivora yang lebih rendah. Struktur komunitas di sawah dengan refugia lebih seimbang, INP menunjukkan bahwa family Acrididae (56,66%) memiliki peran yang penting dalam ekosistem sawah, dan Indeks Diversitas Shanon-Winner menunjukkan diversitas yang lebih beragam dibanding sawah tanpa refugia.
Uji Deteksi Adulterasi Daging Babi (Sus scrofa domestica) pada Bakso Metode Loop-Mediated Isothermal Amplification Rahma Micho Widyanto; Cleonara Yanuar Dini; Irma Sarita Rahmawati; Sekar Ramadhanti Putri; Amelia Nurdini Rozana; Sakinah Hilya Abida; Yunimar Yunimar
Indonesian Journal of Human Nutrition Vol. 8 No. 1 (2021)
Publisher : Department of Nutrition, Faculty of Health Sciences, Universitas Brawijaya Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.ijhn.2021.008.01.8

Abstract

Composition adulteration in a product is still a serious problem. For that we need a DNA-based test method that is fast, specific and simple. In this study, a rapid test technique with the loop-mediated isothermal amplification method was applied to identify the presence of pork DNA in meatballs. Six primers were used for the identification of mitochondrial DNA cytochrome oxidase subunit II. Samples were prepared in the form of mixed beef meatballs containing pork with a concentration of 50; 5; 0.5; 0.05%, respectively. The results showed that the optimal concentration for primers was 4 pmol inner primers, 2 pmol loop primers, and 1 pmol outer primers. The LAMP reaction showed a change in color in the meatball sample with the pork mixture to the lowest concentration. The LAMP technique provides a faster test with the final result of on-the-spot color visualization so that it is potential to be developed as a quick detection kit for identification of halal food processed products
Co-Authors Amelia Nurdini Rozana Amin Setyo Leksono Dini, Cleonara Yanuar Haidar Azzamuddin Irma Sarita Rahmawati Rahma Micho Widyanto S. Fatiyatur Rahmah Sekar Ramadhanti Putri YUNIMAR YUNIMAR Yuris Setyadin