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Heddy Julistiono
Research Center for Biology, Indonesia Institute of Sciences (LIPI), Cibinong, Bogor 16911, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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 1 
IDENTIFICATION OF YEASTS ISOLATED FROM GUNUNG HALIMUN NATIONAL PARK*[Identifikasi Khamir pada Taman Nasional Gunung Halimun] Atit Kanti; Heddy Julistiono; I Made Sudiana
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i1.1177

Abstract

Dua puluh sembilan isolat khamir diisolasi dari tanah Taman Nasional Gunung Halimun. Sumber isolat berasal dari batang pohon lapuk, akar lapuk yang diambil dari Gunung Botol, Cikaniki, dan Cipta Rasa untuk dipelajari aspek taksonominya. Berdasarkan atas karakter morfologi dan fisiologi, isolat-isolat tersebut digolongkan kepada kelompok ascomyceteous, basidiomyceteous dan imperfect khamir. Selanjutnya ketiga golongan tersebut dimasukkan ke dalam sepuluh kelompok (Kelompok 1 sampai X). Dari 29 isolat tersebut, 7 isolat dimasukkan ke dalam kelompok I diindentifikasi sebagai Debaryomyces hansenii, 6 dalam kelompok II sebagai Candida sp, 2 dalam kelompok III sebagai Pichia membranafaciens, 5 isolat dalam kelompok IV sebagai Candida galacta, 1 dalam kelompok V sebagai Candida sake, 4 dalam kelompok VI sebagai Cryptococcus humicolus, 1 dalam kelompok VII sebagai Rhodotorula minuta, 1 dalam kelompok VIII sebagai Candida sp, 1 dalam kelompok Candida sp, dan 1 dalam kelompok X dalam Candida sp. Macam sampel tampaknya tidak berpengaruh kepada keragaman jenis khamir seperti ditunjukkan oleh jenis yang sama diisolasi dari berbagai jenis sampel. Dari banyaknya jenis khamir yang diisolasi menunjukkan bahwa keragaman jenis khamir di Taman Nasional Gunung Halimun tergolong tinggi.
THE COMMUNITY OF SOIL YEASTS IN GUNUNG HALIMUN NATIONAL PARK Atit Kanti; I Made Sudiana; Heddy Julistiono
BERITA BIOLOGI Vol 5, No 6 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i6.1081

Abstract

Fifty-two isolates were isolated from Gunung Halimun National Park on the basis of morphological and some physiological characteristics.Those isolates were belonged to three groups namely, ascomycetous, basidiomycetous and imperfect yeasts.Rhodotolum sp.was only found in Ciptarasa site at 1500 m asl, ascomycetous yeasts was only isolated from deteoretic root in Gunung Botol site, while Candida sp.(small globose shaped cells) was only isolated from soil at 1800 m asl of Gunung Botol site. Type of plant species appeared has no effect on yeasts diversity as shown by similar yeasts diversity was observed at rhizosphere soil of three dominating plant (Schima waallichii, Castanopsis javanica and Altingia excelsa) at Cikaniki study site.
KAJIAN KERAGAMAN GENETIK BURUNG KAKATUA TANIMBAR (Cacatua goffini, Finsch) MENGGUNAKAN PENCIRI "RAPD" Dwi Astuti; Siti N. Priyono; Heddy Julistiono; Dedy Duryadi
BERITA BIOLOGI Vol 4, No 2&3 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i2&3.1277

Abstract

The study was conducted to analyse genetic diversity of Goffini Finsch Cockatoo (Cacatua goffini Finsch) bird using RAPD (Random Amplified Polymorphic DNA) marker.PCR (Polymerase Chain Reaction) was performed on DNA samples extracted from 14 birds,using 18 random 10-merprimers and 2 random 12-merprimers. Fourteen out of 20 primers (70 %) successfully amplified DNAfragmens and 11 out of 14 primers (78,57 %) generated 1-2 specific alkies.The result clearly demonstrated that the RAPD marker allows for genetic diversity analyses of these birds in efficiently. Tree of relationship among 14 birds showed that there were two groups in the population ofGoffin 's Cockatoo.
TOKSISITAS GLISEROL ATAU SUKROSA PADA SEL KHAMIR accharomyces cerevisiae YANG DISEMPAN PADA SUHU RENDAH BEKU Heddy Julistiono
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i4.1123

Abstract

The effect of cryoprotectants glycerol and sucrose on cell viability and fermentation rate of Saccharomyces cerevisiae after freezing (-30 °C) and Chawing (30 °C) were studied.Both freezing and thawing were done rapidly. The mortality of cells treated with low concentrations of cryoprotectans (2.5, 5, and 10 %) after 15 and 30 days of cryopreservation, was remarkably higher than that of control and higher concentration (20% and 40%).Glycerol or sucrose with concentration of 20 % and 40 % protected cells from severe mortality only after 90 days of cryopreservation.Fermentation rate of cells treated with 20 % or 40 % of the two cryoprotectants were higher than that of control after 60 and 90 days of cryopreservation.The data indicated that in certain circumstance cryoprotectant could be toxic for the cells during freezing and thawing.Since biomembrane is not permeable to sucrose, therefore we proposed that target of sucrose toxicity may be extracellular, whereas glycerol, which penetrate cells,targets of the toxicity may be both extracellular and intracellular domains.Interaction between cryoprotectant and cell membranes is discussed.
ANALISIS VARIASI GENETIK Saccharomyces cerevisiae Dl TAHAN ETANOL DENGAN RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) Heddy Julistiono; Titin Yulineri; Sukamto Hanjono
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1240

Abstract

Genetic variations among 3 cultures, which were treated with or without Mn of Saccharomvces cerevisiae D1, were analyzed using RAPD (Random Amplified Polymorphic DNA ) technique. The Mn-treatment of three cultures were as follows: the culture KMn was D1 strain, the culture Mn+ was D1 strain colony survived in ethanol 20%, which was previously treated with 0,5 mU MnSOt and the culture Mn- was a D1 colony survived in ethanol 20% without MnSOi treatment. Polymorphism of total DNA of the three cultures may indicate that mutation may occur in cells which were tolerant to ethanol. The locus or base change was not identified. However, since the oxygen uptake rate of the three cultures at catabolite derepression state were identical,the results suggest that the locus may not be in mitochondrial DNA encoding respiratory chain proteins. The relation between DNA polimorphic and ethanol tolerant cell is still to be clarified.
Co-Authors Atit Kanti Dwi ASTUTI I Made Sudiana Siti N. Priyono Sukamto Hanjono TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Titin Yulineri