Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal JURNAL TEKNOLOGI LINGKUNGAN Jurnal Ilmu Kefarmasian Indonesia
Ira Djajanegara
Badan Pengkajian dan Penerapan Teknologi
Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Medicine & Pharmacology Public Health

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
PEMANFAATAN LIMBAH BUAH PISANG DAN AIR KELAPA SEBAGAI BAHAN MEDIA KULTUR JARINGAN ANGGREK BULAN (Phalaenopsis amabilis) TIPE 229 Ira Djajanegara
Jurnal Teknologi Lingkungan Vol. 11 No. 3 (2010)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.15 KB) | DOI: 10.29122/jtl.v11i3.1182

Abstract

Coconut water and rotten banana fruits are commonly found in traditional markets as organic wastes. One way to overcome the problems caused by these organic wastes is to convert these unuseful matter into an important and economically useful matter by using them as components of tissue culture media. One important commodity that is usually propagated by tissue culture is Phalaenopsis orchid type 229 (Phalaenopsis amabilis). Therefore, it would be more benefit to substitute the expensive chemicals with organic wastes such as coconut water and banana puree.In this experiment, addition of coconut water and banana pure to the minimum media containing commercial fertilizer red Polyhyponex, sucrose and commercial agar did not show any inhibition of Phalaenopsis orchid plantlet growth. This probably caused by sufficient macro and micro nutrients provided by those organic matter and Polyhyponexfertilizer. Moreover, addition of 100 mL/L of coconut water and 100 mg/L banana puree gave the optimum leaf and adventitious shoot formation. On the other hand, addition of 150 mL/L coconut water gave the optimum height and root formation. In this case,growing Phalaenopsis orchid plantlet should be done in 2 subculture period. The first subculture is to obtain maximum amount of leaf and shhot formation while the second subculture is to obtain optimum height and root formation.Key words : Waste, coconut water, banana puree, tissue culture, Phalaenopsis orchi
Pemakaian Sel HeLa dalam Uji Sitotoksisitas Fraksi Kloroform dan Etanol Ekstrak Daun Annona squamosa IRA DJAJANEGARA; PRIO WAHYUDI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 7 No 1 (2009): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1336.734 KB)

Abstract

Custard apple (Annona squamosa) is a favorite fruit in Indonesia. The leave of this plant is used and known for long time as an astringent, antiinflammatory, birth control, and antitumor agents. Previous experiments on toxicity using brine shrimp lethality test (BSLT) method indicated that the chloroform fraction of custard apple leaves was toxic to the shrimp larvae with LC50 value at 1.77µg/ml. This experiment was carried out to investigate further the possible role of custard apple leaves as an anticancer agent using HeLa cell line. Experiment was initiated by extracting the leaves using 70% ethanol by means of maceration. The macerate was then fractionated further using chloroform as solvent. The ethanol fraction and chloroform fraction were used in cytotoxicity assay using (cervix cancer) HeLa cell line. Cytotoxicity assay Was done using direct counting method. The amount of living cells were observed and counted, death percentage Was then determined and probit analysis Was applied to determine the LC50 value. LC50 value for ethanol fraction from the leaves of custard apple (Annona squamosa) Was 7.695 µg/ml While for chloroform fraction was 4.547 µg/ml based on cytotoxicity analysis using HeLa cells. This experiment concluded that theleaves of custard apple (Annona squamosa) has cytotoxicity toward HeLa cell lines which confirmed the previous toxicity experiment using brine shrimp lethality test (BSLT) method. Overall, this experiment indicated that the leaves of custard apple (Annona squamosa) have anticancer activity.
Uji Sitotoksisitas Ekstrak Etanol Herba Ceplukan (Physalis angulata Linn.) terhadap Sel T47D secara In Vitro IRA DJAJANEGARA; PRIYO WAHYUDI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 8 No 1 (2010): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1634.959 KB)

Abstract

Cutleaf groundcheny (Physalis angulata Linn.) is a popular traditional medicine. This experiment was conducted to investigate the possible role of the herbal extract as an anticancer agent using T47D breast cancer cell line. Cytotoxicity assay was caried out by direct counting method. Breastcancer T47D cell lines Were treated with 70% ethanol extract from cutleaf groundcherry fruit with serial dilution of 2000, 1000, 500, 250, 125, 62.5, and 31.25 µg/ml for 24 hours of incubation at 37°C As a positive control doxorubicin was also tested on the breast cancer T47D cell lines with serial dilution of 20, 10, 5, 2.5, 1.25, 0.625, 0.312, and 0.156 µg/ml for 24 hours of incubation at 37°C The amount of living cells were observed and counted, death percentage was then determined and probit analysis was used to determine the LC50 value. The LC50 value for ethanol extract from the fruits was 28,02 µg/ml While doxorubicin as the positive control was 0,113 µg/ml. It is concluded that the cutleaf groundcherry (Physalis angulata) fruits have a cytotoxic activity toward T47D (breast cancer) cell lines which confirmed previous toxicity experiment using brine shrimp lethality test (BSLT) method.
Co-Authors PRIO WAHYUDI Priyo Wahyudi