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All Journal JURNAL TEKNOLOGI LINGKUNGAN
Ninu Setianingrum
Lembaga Ilmu Pengetahuan Indonesia
Environmental Science

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AKTIVITAS AKTINOMISETES DARI BANGKA-BELITUNG KOLEKSI BIDANG MIKROBIOLOGI, PUSLIT BIOLOGI- LIPI DALAM MEMPRODUKSI ENZIM KITINASE yati Sudaryati Soeka; Evi Triana; Ninu Setianingrum
Jurnal Teknologi Lingkungan Vol. 11 No. 3 (2010)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (431.075 KB) | DOI: 10.29122/jtl.v11i3.1187

Abstract

The aim of the research was to know the capability of actinomycetes isolate from Bangka Belitung, which stored at Microbiology-LIPI Culture Collection, in producing chitinase enzyme. This isolate which could produce chitinolitic enzyme, signed by clear zone at medium contain 1% chitine. The chitinase activity of the isolate which incubated for 1-7 days in the room temperature was analyzed by spectrophotometer in λ 584 nm. The result of this experiment was highest chitinase activities with incubated for 3 days, were 1.66 . 10-2 U/ml. Maximum chitinase activities was found at 1% starch soluble substrate 2.83 . 10-2, pH 8.0 and at 50°C condition were 9.3 . 10-2 and 12.98 .10-2 U/ml respectively.Key words : chitinase, clear zone, spectrophotometer
Co-Authors Evi Triana yati Sudaryati Soeka