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Nunik Sulistinah
Lembaga Ilmu Pengetahuan Indonesia
Environmental Science

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KEMAMPUAN Flavobacterium sp NUB1 DALAM MENGGUNAKAN ALIFATIK NITRIL UNTUK PERTUMBUHANNYA Nunik Sulistinah
Jurnal Teknologi Lingkungan Vol. 11 No. 3 (2010)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (552.399 KB) | DOI: 10.29122/jtl.v11i3.1188

Abstract

A bacteria isolate capable utilizing 1% (v/v) acetonitrile and butironitrile as thesolesource of carbon and nitrogen was isolated from industrial effluents ofPTPetrokimia-Gresik and identified as Flavobacterium sp NUB1. The bacteriaisolatewas able to grow in both acetonitrile and butironitrile at concentrationofup to 4% (v/v). The highest growth was reached at 1% concentration ofacetonitrileand butironitrile, but the bacteria isolate was not able to grow onacrilonitrile.The specific growth rate (μ) of the isolate was 0,029 h-1. Themajorobjective of this study was to explore the abilities of the isolate to utilizesomealiphatic nitriles and then further evaluate the metabolite product of thenitriledegradation.Key words: aliphatic nitrile, biodegradation, Flavobacterium sp. NUB1
KONSORSIA BAKTERI PENGURAI SIANIDA YANG DI ISOLASI DARI BUANGAN INDUSTRI PENGOLAHAN EMAS Nunik Sulistinah; Bambang Sunarko
Jurnal Teknologi Lingkungan Vol. 11 No. 3 (2010)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.294 KB) | DOI: 10.29122/jtl.v11i3.1191

Abstract

Bacterial consortium capable of growing and utilizing cyanide as a source of nitrogen were isolated from effluent of gold mining industry. The isolation was conducted using liquid enrichment medium with potassium cyanide and glucose as nitrogen and carbon source, respectively. These consortium could tolerate and were able to grow on KCN at concentration of up to 1000 ppm. Bacterial consortium LP3 were also able to degrade potassium cyanide and ammonium as product of the degradation. The degradation rate was 9,0μM per minute. The cyanide-degrading bacteria found in this consortium were identified as Bacillus, Corynebacterium, and Serratia.Keywords: potassium cyanide, nitrogen source, bacterial consortium LP3,degradation
STUDI DEGRADASI DIBENZOTHIOPHENE OLEH Sphingomonas paucimobilis BAKTERI INDIGENOUS MUARA BARU-TELUK JAKARTA Nunik Sulistinah; Rini Riffiani
Jurnal Teknologi Lingkungan Vol. 12 No. 3 (2011)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (872.456 KB) | DOI: 10.29122/jtl.v12i3.1235

Abstract

Dibenzothiophen (DBT) adalah salah satu senyawa Hidrokarbon Aromatik Polisiklik yang dikenal beracun di lingkungan. Sebagian besar PAH bersifat karsinogenik dan persisten di lingkungan. Teknik Biostimulation digunakan untuk mengisolasi bakteri indigen dari Muara Baru yang mampu mendegradasi DBT. Tujuan utama dari penelitian ini untuk mengisolasi dan meneliti bakteri laut yang dipilih untuk mendegradasi senyawa DBT. Hasil penelitian menunjukkan bahwa bakteri isolat M4 (Sphingomonas paucimobilis) dapat tumbuh optimal pada 30 oC dengan 1,6 X 109 sel / ml dan waktu penggandaan (td) adalah 6 jam. Pertumbuhan Sphingomonas paucimobilis pada konsentrasi 2% NaCl adalah 2,6 X 109 dengan Waktu penggandaan 11 jam. Proses biodegradasi DBT menunjukkan bahwa Km dan Vmaks untuk KNO3 adalah 0,0307 jam-1 dan 12,27 mglt-1 h-1. KNO 3 dan NH4NO3 adalah sumber yang cocok dari nitrogen untuk mempercepat kecepatan biodegradasi Sphingomonas paucimobilis. Efisiensi degradasi mereka adalah 62,5% dan 57,6%.
Co-Authors Bambang Sunarko Rini Riffiani