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All Journal Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences
Widya Pintaka Bayu Putra
Research Centerfor Biotechnology Indonesian Institute of Sciences
Veterinary

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Genotyping in the Insulin-like Growth Factor 1 (IGF1/SnaBI) gene of Pasundan cattle with PCR-RFLP method Widya Pintaka Bayu Putra; ST Nugraheni; Y Irnidayanti; S Said
Jurnal Ilmu Ternak dan Veteriner Vol 23, No 4 (2018): DECEMBER 2018
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (110.089 KB) | DOI: 10.14334/jitv.v23i4.1862

Abstract

Gen Insulin-like Growth Factor 1 (IGF1) pada mamalia berfungsi untuk mengontrol pertumbuhan tulang dan otot. Oleh karena itu gen IGF1 banyak digunakan sebagai salah satu kandidat gen untuk seleksi ternak. Penelitian ini bertujuan untuk mengidentifikasi genotip gen IGF1 (ekson 1) menggunakan metode PCR-RFLP dengan enzim restriksi SnaBI (TAC*GTA). Sebanyak 90 ekor sampel DNA sapi Pasundan dari Kabupaten Ciamis dan Pangandaran, Jawa Barat telah digunakan pada penelitian ini. Hasil penelitian ini menunjukkan bahwa seluruh sampel yang dianalisis memiliki genotip CC dengan alel C sebagai alel yang umum pada gen IGF1/SnaBI. Genotip CC yang diperoleh pada penelitan ini disebabkan karena terdapat mutasi transisi pada posisi basa ke g.218T/C (GenBank: KF202095). Mutasi ini menyebabkan perubahan asam amino dari methionine (AUG) menjadi valine (GUG). Disimpulkan bahwa gen IGF1/SnaBI pada sapi Pasundan bersifat monomorfis dan tidak dapat digunakan untuk seleksi molekuler.
Leptin Gene as Potential Gene for Molecular Selection on Cattle in Indonesia Widya Pintaka Bayu Putra; Rhesti Indriastuti
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 27, No 3 (2017): September 2017
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.284 KB) | DOI: 10.14334/wartazoa.v27i3.1579

Abstract

Genetic improvement in beef and dairy cattle which can increase meat and milk production can be conducted through livestock selection based on molecular technique using leptin gene. The leptin gene is located on the fourth chromosome and consists of three exons and two introns. The leptin gene serves to produce the leptin hormone secreted by fat tissue. High concentrations of leptin hormone can increase feed intake and body metabolism thereby increasing livestock productivity. This paper describes the leptin gene including structure, mechanism of leptin hormone and polymorphisms in cows. These polymorphisms occur in the promoter, intron and exon sections associated with body and carcass weight and milk production in some cattle breed. Therefore, leptin gene can be used for selection towards increasing cattle productivity. Utilization of leptin gene can be done by single nucleotide polymorphism (SNP) identification on the whole structure of leptin gene and to observe its effect on characteristics of cattle production in Indonesia.
Co-Authors Rhesti Indriastuti S Said ST Nugraheni Y Irnidayanti