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Journal : Jurnal Ilmu Ternak Veteriner

Effects of zinc and calcium minerals intake on cadmium accumulation in the liver of broiler chicken Sri Rachmawati; Darmono .; Zainal Arifin
Jurnal Ilmu Ternak dan Veteriner Vol 4, No 3 (1999): SEPTEMBER 1999
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.381 KB) | DOI: 10.14334/jitv.v4i3.161

Abstract

Cadmium is a heavy metal which can accumulate in animal tissues, may have an effect the growth of chicken, causes a residual problems and is danger for human consumption. Study on evaluating the effect of mineral zinc (Zn) and calcium (Ca) intake on cadmium (Cd ) accumulation in the liver of broilers and their growth was carried out. One hundred and eighty of day old chicks (DOC) were divided into 6 groups, group I was used as control, group II, III, IV, V and VI were given feed containing of 5 ppm Cd, 5 ppm Cd and 10 ppm Zn, 5 ppm Cd and 15 ppm Zn, 5 ppm Cd and 10 ppm Ca, 5 ppm Cd and 15 ppm Ca respectively in their feed. Cd, Zn and Ca were added as CdCl2, ZnCl2 and CaCl2. During the experiment, at week -0, -1,-2,-3 and -4, five chickens from each group were weighed and then they were slaughtered and livers were collected. The liver samples were analyzed for Cd content by Atomic Absorption Spectrophotometer. The result indicated that Cd exposed to chickens caused the accumulation of that Cd in their liver. The addition of 15 ppm Zn in feed reduced the accumulation of Cd in liver significantly (P<0.05). However the addition of Ca in chicken feed gave greater effect in reducing the Cd accumulation in liver compared to that of Zn supplementation. 10 ppm Ca and 15 ppm Ca in chicken feed caused the accumulation of Cd in liver chicken significantly decreased (P<0.05). Ca supplementation also caused of increasing body weight of chickens. The body weight of five weeks old chickens given 15 ppm Ca in their feed as in average of 1,820 grams is higher than that of chickens in control group, which was 1,761 grams. Whereas the body weight of chickens in group IV, which was given with 15 ppm Zn in their diet was in average of 1,745 grams. It was concluded that Zn and Ca can reduce the accumulation of Cd in broiler chicken liver.   Key words : Zinc, calcium, accumulation, cadmium
Sambiloto (Andrographis paniculata Nees.) for reducing aflatoxsins contamination in commercial chicken feed Sri Rachmawati; zainal Arifin; Paderi Zahari
Jurnal Ilmu Ternak dan Veteriner Vol 4, No 1 (1999): MARCH 1999
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.78 KB) | DOI: 10.14334/jitv.v4i1.139

Abstract

Indonesian climate condition is suitable for growing of mold such as Aspergillus flavus. The mold grown in feed and feed ingredients cause aflatoxins contamination of the feed. Sambiloto has been reported to reduce the growth of Aspergillus flavus and aflatoxins production in feed isolate. The aim of the study was to find the used of sambiloto in reducing aflatoxins contamination in commercial chicken feed. The feed was homogenously mixed then divided into 4 groups with 3 replicates. The samples were treated with sambiloto 0.04%, 0.08% and 0.16% respectively into the feed, except for the control feed sambiloto was not added. Into these feed 4 ml of Aspergillus flavus suspension was also added. Then the feed in each group was incubated at room temperature for 10 days. Samples were collected from each group at days 0, 5 and 10 of incubation times for aflatoxins analysis. Samples were extracted and aflatoxins were detected by High Performance Liquid Chromatography (HPLC). Observation results indicated that sambiloto concentration of 0.16% in feed can inhibit the total aflatoxins production of 16.46% and gave the inhibitation percentage of aflatoxin B1 of 45.39%. Statistical test showed that the used of sambiloto in feed for 5 days incubation times gave significant result in reducing aflatoxins production. However the 10 days incubation times of the feed with sambiloto gave in significant result. It is suggested that to get optimum inhibition of aflatoxins production, sambiloto added in feed should be higher than 0.16%.   Key words : Feed, aflatoxin, sambiloto