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All Journal Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Menara Perkebunan
Hasim MUNAWAR
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Karakteristik antibodi anti Ganoderma sp. yang dihasilkan dengan menggunakan jenis dan sumber antigen yang berbeda [Characteristic of antibodies againt Ganoderma sp produced from different types and sources of antigens] Irma KRESNAWATY; Kholis A AUDAH; Hasim MUNAWAR; Happy WIDIASTUTI
E-Journal Menara Perkebunan Vol 85, No 1 (2017): April, 2017
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (450.436 KB) | DOI: 10.22302/iribb.jur.mp.v85i1.239

Abstract

Basal stem rot (BSR) disease caused by  Ganoderma sp. is the most important disease in oil palm plantations.The effectivity of BSR control depends on early detection of this disease. The earlier the disease is known, the severity of damage could be prevented. Therefore, technology for early detection of Ganoderma infection is very important. Immunochromatographic techniques based on the reaction of antigens and antibodies can be developed for detection of Ganoderma sp infection. The objective of the study was to produce antibodies using different Ganoderma sp. In this study, immunoglobulin Y ( IgY ) against Ganoderma sp produced in chicken eggs was used as the source of antibodies. Laying hens were immunized with several types of Ganoderma sp. because it is known to have genetic variations. The source of Ganoderma sp. isolates were mycelium and exudates. The polyclonal IgY antibodies produced economically and abundantly.  The antibodies derived from the mycelium showed more consistent results compared with those derived from the exudates. In addition, the antibodies derived from Ganoderma sp of Cimulang and Bekri showed higher reactivity  with some of the antigens compared to those from Cisalak Baru (CSB). The characteristics and the protein profiles of antibodies produced using Cimulang, Bekri  and Cisalak Baru isolates were vary in term of,  sensitivity and amino acid compositions
Pengembangan Indirect Dipstick ELISA untuk Deteksi Aflatoksin B1 pada Pakan dan Jagung Sri Rachmawati; Prima Mei Widiyanti; Hasim Munawar
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 2 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.2.129

Abstract

Enzyme Linked Immunosorbent Assay (ELISA) is an immunological method can be used to analyze aflatoxin B1 (AFB1) in feed. ELISA technique must be done by using an instument (ELISA reader) which is not effective when used in the field. Therefore, the simple ELISA technique is needed such as indirect dipstick ELISA (d-ELISA). The aim of research is to develop AFB1 screening method using   d-ELISA. The research is focusing on  development and validation of indirect d-ELISA, and its application on sample of feed and corn. The results showed that the best coating time for antigen AFB1-BSA (0,4 ug/ml) is 24 hours, reaction time for antibody anti AFB1 (1/800) and AFB1 standard is 15 minutes, and reaction time for goat anti rabbit-HRPO conjugate 1/2500 and substrate of orthodiasianin (ODN) is 20 minutes. The results of indirect d-ELISA on 22 samples are found that 7 samples are contaminated by AFB1 with concentration above 20 ng/g and 7 samples are contaminated by AFB1 with concentration in the range of 0 – 20 ng/g. Finally, it is concluded that the indirect d-ELISA is applicable to be used in the fields.
Karakteristik antibodi anti Ganoderma sp. yang dihasilkan dengan menggunakan jenis dan sumber antigen yang berbeda [Characteristic of antibodies againt Ganoderma sp produced from different types and sources of antigens] Irma KRESNAWATY; Kholis A AUDAH; Hasim MUNAWAR; Happy WIDIASTUTI
Menara Perkebunan Vol. 85 No. 1 (2017): 85 (1), 2017
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v85i1.239

Abstract

Basal stem rot (BSR) disease caused by  Ganoderma sp. is the most important disease in oil palm plantations.The effectivity of BSR control depends on early detection of this disease. The earlier the disease is known, the severity of damage could be prevented. Therefore, technology for early detection of Ganoderma infection is very important. Immunochromatographic techniques based on the reaction of antigens and antibodies can be developed for detection of Ganoderma sp infection. The objective of the study was to produce antibodies using different Ganoderma sp. In this study, immunoglobulin Y ( IgY ) against Ganoderma sp produced in chicken eggs was used as the source of antibodies. Laying hens were immunized with several types of Ganoderma sp. because it is known to have genetic variations. The source of Ganoderma sp. isolates were mycelium and exudates. The polyclonal IgY antibodies produced economically and abundantly.  The antibodies derived from the mycelium showed more consistent results compared with those derived from the exudates. In addition, the antibodies derived from Ganoderma sp of Cimulang and Bekri showed higher reactivity  with some of the antigens compared to those from Cisalak Baru (CSB). The characteristics and the protein profiles of antibodies produced using Cimulang, Bekri  and Cisalak Baru isolates were vary in term of,  sensitivity and amino acid compositions
Co-Authors HAPPY WIDIASTUTI Irma Kresnawaty Kholis A AUDAH Prima Mei Widiyanti Sri Rachmawati