Garuda - Garba Rujukan Digital

. Sumarningsih

IRCVS

Author-ID : 2823643

Veterinary

Published : 3 Documents Claim Missing Document

Claim Missing Document

Articles

Endemicity of avian influenza in ducks living around commercial layer farms Simson Tarigan; Risa Indriani; . Sumarningsih
Jurnal Ilmu Ternak dan Veteriner Vol 20, No 4 (2015): DECEMBER 2015
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The progenitors of all avian influenza viruses are generally derived from ducks or other waterfowl that have undergone mutation and adaptation to become pathogenic in chickens or other poultry. Investigation of the presence of avian influenza viruses in ducks especially those living around chicken farms is, therefore, important. Serum from 54 ducks and 51 Muscovy ducks living around commercial layer farms in the districts of Cianjur and Sukabumi were collected in March - April 2014. The indication of AI-virus infection in those birds was based on an array of serological tests including competitive and indirect ELISAs for antibody to nucleoprotein, MM2e ELISA for antibody to M2e, HI test, ELISAs and dot blot for antibodies to haemagglutinin, and dot blot assay for antibodies to neuraminidase. Recombinant Haemagglutinins (H1-H13 and H15), recombinant neuraminidases (N1, N2, N7 and N9) and recombinant influenza-A nucleoprotein were used in the indirect ELISAs and dot blot assays. As many as 63% of duck samples and 13% Muscovy-duck samples were serologically positive to nucleoprotein, and 62% of the nucleoprotein-seropositive ducks were also positive to M2e. The high seroprevalence of AI in the ducks living around commercial poultry farms suggested that application of strict biosecurity measures on those farms is still needed. Based on the results of the ELISA and dot blot assays, AI virus subtypes H9N2 and H5N2, in addition to H5N1, were suspected to be circulating in those ducks. Further confirmation by virus isolation, however, is required because H9N2 and H5N2 subtypes have yet been unknown Indonesia and both the subtypes can cause serious disease in poultry.Key Words: Duck, Immunoassay, Avian Influenza, H5N1, H5N2, H9N2

Generation of scFv-Monoclonal Antibody Avian Influenza Diagnostic tests Simson Tarigan; . Sumarningsih
Jurnal Ilmu Ternak dan Veteriner Vol 24, No 1 (2019): MARCH 2019
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The need for rapid diagnostic tools or point- of- care diagnostic tests for Avian Influenza in Indonesia is very high and the price of these imported diagnostic tools is very expensive. As a result, a large budget requires to provide the needs. The main component of a rapid diagnostic tool is the monoclonal antibody (mAb) specifically recognized influenza viruses. The objective of this study was to produce mAb that can recognize all subtypes of Avian Influenza viruses using the phage display technology. Influenza-A focused scFv commercial library was panned using alternating recombinant H1N1 NP and H5N1 virions. Whereas, bacteriophages bound to the panning baits were eluted with serum from H5N1-infected chickens. Phagemid from suppressor E. coli (TG1) infected with bacteriophage displaying anti-NP on its surface was isolated and then transformed into a non-suppressor E. coli (HB2151) to express NP-scFv. Monoclonal NP-scFv antibody with a molecular weight of about 27 kDa was purified from the culture supernatant using a nickel-chromatography column. The amount of pure NP-scFv obtained was around 1.2 mg /L culture. As an additional component for its use in immunoassays, antibody to NP-scFv was produced in rabbits. The generating polyclonal antibody recognized the NP-scFv specifically and sensitively. The anti-NP-scFv monoclonal antibody and the anti rabbit scFv polyclonal antibody produced in this study are envisaged appropriate for the development of diagnostic tools for point-of-care for Avian Influenza.

Potency of antigenic and serologic tests based on CNTKCQTP linear epitope on H5N1 haemagglutinin for Avian Influenza Simson Tarigan; . Sumarningsih
Jurnal Ilmu Ternak dan Veteriner Vol 21, No 1 (2016): MARCH 2016
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Rapid diagnostic tools or point-of-care (POC) test is needed in the effort to control and eradicate the high pathogenic avian influenza (HPAI) H5N1 in Indonesia. Accuracy of a POC test is determined by the specificity of antibodies, which is the main component of a POC test. Recently a linear epitope, CNTCKQTP epitope, located at 274-281 amino acid residue of H5 hemagglutinin has been confirmed to be present all clade of H5N1 viruses. This study aimed at producing and evaluating the reactivity of a monospecific, polyclonal antibody against the epitope. The Antibody was produced by immunising a goat with the peptide in the form of multiple antigen peptide (MAP). The specificity of the antibody was estimated by assaying its reactivity against influenza virus subtypes H3N3, H4N4, H5N1, H6N5, H7N7, H9N2, H10N7 and H11N9; and recombinant hemagglutinins H1-H12, H14 and H15 with ELISA and immunoblot. The results of the assay showed that CNTKCQTP antibody was not specific for H5 haemagglutinin because it cross-reacted with other haemagglutinins especially H7, H8 and H9. The potential of the peptide containing the epitope, GNCNTKCQTPMGAINSS. as an ELISA reagent for assaying H5 antibodies in chickens previously vaccinated and challenged with the H5N1 virus was also evaluated in this study. In contrast, the results of previous studies, the ELISA using GNCNTKCQTPMGAINSS as coating antigen was not sensitive in detecting antibody to haemagglutinin H5 in chickens.Key Words: AI Virus, Hemagglutinin H5, CNTKCQTP Epitope, MAP, Immunoassay

Title

Found 3 Documents
Search

Abstract

Abstract

Abstract

Title Search

Found 3 Documents Search

Abstract

Abstract

Abstract

Title

Found 3 Documents
Search