Triana Susanti
Indonesian Research Institute for Animal Production

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Single nucleotide polymorphism of prolactin gene exon two in ducks of Pekin, Mojosari and Pekin Mojosari crossbred Irma .; Cece Sumantri; Triana Susanti
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 2 (2014): JUNE 2014
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v19i2.1038

Abstract

Prolactin gene plays crucial role in the reproduction and egg production of birds. The objectives of this study were to characterize single nucleotide polymorphism in partial intron and coding region of duck prolactin gene. Blood samples were collected from 168 ducks consisted of 19 Pekin, 36 Mojosari, and 113 of their crossbreds collected from Indonesian Research Institute for Animal Production (IRIAP). Primer pairs for the coding regions in prolactin gene were self designed based on the duck genomic sequence database (GeneBank: AB158611.1). PCR products based on DNA of prolactin gene exon two was amplified approximately 400 bp. There is one base insertion of Adenin at the position of 2001 bp intron two region of duck prolactin. Homology test based on BLAST method indicated 99% identity with duck refference (Code Access GeneBank: AB158611.1). Adenin composition in all of duck samples was higher than refference. Triplet hydrogen bonds between Guanine and Cytosin pairs was higher than those at duplet hydrogen bonds between Adenine and Thymine. All duck samples were homozigous and monomorphyc.
Breeding Strategy of Local Muscovy to Support the Development of Meat Type Duck Industry Triana Susanti
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 31, No 3 (2021): September 2021
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (619.796 KB) | DOI: 10.14334/wartazoa.v31i3.2671

Abstract

Muscovy duck is one of the genetic resources of waterfowl that spreads almost all over Indonesia. Muscovy duck is classified as meat-producer waterfowl, because of its relatively large body weight and better meat quality compared to other waterfowl. Therefore, cross breeding between Muscovy and ducks were often conducted by farmers to produce offspring with large body weight in order to anticipate the higher demand for duck meat. If this crossing pattern has been widely adopted by breeders, it will require a larger number of Muscovy duck. Whereas, currently the population of Muscovy is relatively low and no breeding system exists both at the community farm level or industrial level. Therefore, a development program of local Muscovy duck, especially its breeding program needs to be done immediately. As an information material to facilitate the preparation of the program, this paper describes factors that affect the breeding program including production performance and reproduction, as well as the breeding strategy of Muscovy duck as candidates of male line to form special type of meat type ducks through 3 groups of livestock breeds in the shape of a conical triangle at the top. The top position was the pure stock (nucleus), the middle was the parent stock (multiplier) and the bottom was the commercial stock. The genetic quality of livestock flows from the top position to the bottom and  cannot  be the other way around, because the breeding system is closed in the pure stock (nucleus).