Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences
Eni Kusumaningtyas
Indonesian Research Centre for Veterinary Science
Veterinary

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Molecular Profile of Trichophyton mentagrophytes and Microsporum canis based on PCR-RFLP of Internal Transcribed Spacer Dwi Endrawati; Eni Kusumaningtyas
Jurnal Ilmu Ternak dan Veteriner Vol 26, No 1 (2021): March 2021
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v26i1.2546

Abstract

Trichophyton mentagrophytes and Microsporum canis are dermatophytes fungi which commonly infect animal and human. Conventional and molecular methods were used for identification of the fungus. The region of internal transcribed spacer (ITS) has a high probability for fungal identification. PCR-RFLP was reported as a useful method to differentiate dermatophytes fungi. The objective of the study was to compare molecular profile of T. mentagrophytes and M. canis based on the result of ITS fragment digestion using Dde I, Hinf I and Mva I. The molds were isolated from skin scrapping of 18 animals which showed dermatophytosis lesion. The isolated molds were grown on agar plate for 14 days of incubation at 37oC and then identified based on macro and microscopic morphologies. Amplification of chitin synthase gene was used for confirmation and separation of dermatophytes from other fungi. ITS fragment was amplified and then digested using restriction enzymes Dde I, Hinf I and Mva I. The result showed that digestion products from ITS fragment of T. mentagrophytes and M. canis were different. The fragment 159 bp from Dde I, 374 bp from Hinf I and 89 bp from Mva I were present in T. mentagrophytes but absent in M. canis.   Based on these results, specific RFLP profile of digestion ITS region by Dde I, Hinf I and Mva I can be used as a specific marker for species of dermatophytes fungi.
Application of Peptide for Improving Animal Health and Livestock Productivity Eni Kusumaningtyas
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 28, No 2 (2018): June 2018
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.116 KB) | DOI: 10.14334/wartazoa.v28i2.1783

Abstract

The use of antibiotics as growth promoter has been banned in Indonesia. One potential alternative antibiotic is peptide compounds. Peptides composed of several amino acid monomers that bind through peptide or amide bonds. Peptides are found in natural or synthetic forms that can be used to improve animal health and livestock productivity. This paper discusses the application of peptides in animal health and livestock productivity and the possibility of their utilization in Indonesia. Antimicrobial peptides are used to overcome bacterial infections, especially that are resistant to antibiotics and to preserve semen. Peptide vaccine can be used to replace conventional vaccines, especially for malignant viruses such as foot and mouth disease virus (FMD). Other peptides are used for infectious diseases and diabetes diagnostic tool in animals, medical treatment and animal reproduction system. Some peptides can be isolated from venom and have several benefits as an alternative fiber sutures for surgery and pain relief. Peptide-based drugs are metabolized faster than other small molecules, therefore, reducing the risk of contamination in livestock products.
Co-Authors Dwi Endrawati