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Estimated analysis criteria of hatched weight and body weight 12 weeks of Kampung chicken selection Fitra Aji Pamungkas
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 4 (2005): DECEMBER 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Genetic parameter estimation for production traits are important in designing genetic selection program for Kampung chicken. The aimed of this research is to study heritability, accuracy of selection, and phenotypic and genotypic correlation of hatched weight and body weight at 12 weeks of Kampung chicken. Five hundred and fourteen head of Kampung chicken consist of 13 cocks, 65 hens, and 436 chicks were used in this study. Nested design analysis were used as described by Becker. The heritability estimation of hatched weight was calculated based on paternal half-sib, maternal half-sib, and full-sib corelation and it’s values were 0.35, 0.37, and 0.36 respectively. Heritability of body weight at 12 weeks based on paternal half-sib, maternal half-sib, and full-sib corelation were 0.27, 0.18, and 0.22 respectively. Selection accuracy of hatched weight were 59-61%, and selection accuracy of body weight at 12 weeks were 42 up to 52%. Genotypic and phenotypic correlation of hatched weight and body weight at 12 weeks estimation based on paternal half-sib, maternal half-sib, full-sib corelation were 0.29, 0.78, 0.51, and 0.17 respectively, indicated selection on one trait will affected the response on other traits positively. Key Words: Heritability, Selection, Kampung Chicken

The Use of Vitrification Method For Cryopreservation of Mammalian Oocyte Fitra Aji Pamungkas
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 20, No 3 (2010): SEPTEMBER 2010
Publisher : Indonesian Center for Animal Research and Development

Technique cryopreservation of oocyte is a way to storage, maintenance, and guarantee the survival of frozen cells. Vitrification is a cryopreservation method which is increasingly popular in reproduction but it is still difficult to be done because of the size, shape, and numbers of oocytes, as well as osmotic shock and fractures. The efforts to improve the method and technique vitrification are by reducing the concentration of cryoprotectants, increasing the cooling rate and warming, recovery of meiotic spindles, and the time of fertilization. Vitrification solution consist of 15% (v/v) ethylene glycol, 15% (v/v) dimethylsulfoxide or 1,2-propanediol, and 0.5 mol/L sucrose was less toxic. Therefore, at 37°C, 2 – 3 minutes are usually used for the pretreatment solution and 20 – 30 seconds for exposure to the vitrification solution. In contrast, at room temperature, 5 – 15 minutes are commonly used for pretreatment and 30 – 60 seconds for exposure to the vitrification solution. Warming procedure is performed by direct immersion of the straw into a water bath. Holding the straw in air for 5 seconds before immersion can avoid bursting or performed warming and dilution at 37°C. While the time of fertilization performed at 2 – 3 hours after thawing and incubation for oocyte spindle to recover which is essential for the successful of oocyte cryopreservation program. Key words: Cryopreservation, vitrification, oocyte

Utilization of Assisted Hatching Techniques to Enhance Embryo Implantation Arie Febretrisiana; Fitra Aji Pamungkas
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 27, No 1 (2017): March 2017
Publisher : Indonesian Center for Animal Research and Development

Gestation is the main goal for in vitro fertilization. The embryo that has been developed outside the body will be transferred directly into uterus leading to the process of hatching, implantation, and pregnancy. However, approximately 85% of embryos that have been transferred were failed to implant and it might be caused by hatching failure. Hatching is the process of releasing embryo from zona pellucida. If this process does not occur, it will cause pregnancy failure. Assisted hatching is a mechanism that dealing with thinning, slicing or artificially making holes in the zona pellucida to improve hatching. The process can be applied both in fresh or frozen embryos. This review describes various methods in assisted hatching such as enzymatic, chemical, mechanical, and laser beam as well as their advantages and disadvantages. Generally, some researches show that the technology of assisted hatching can improve the percentage of hatching and implantation of the embryo. However, in spite of the benefits, there are such weaknesses find in the zona pellucida of the embryo that has been manipulated such as toxic hazard medium, the risk of damage to the blastomeres or monozygotic twinning. Therefore, it is advisable to perform assisted hatching in certain cases that tends to face obstacles during the process of hatching such factors as age, embryo quality, the thickness of the zona pellucida and the number of failures in the in vitro fertilization program.

Characteristics and Applications of Nanoparticles in Manipulation of Livestock Reproductive Hormones Fitra Aji Pamungkas; Elizabeth Wina
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 25, No 4 (2015): DECEMBER 2015
Publisher : Indonesian Center for Animal Research and Development

The research on hormone packaged in very small size particles began to develop in recent years. Nanoparticles are defined as particulate dispersions or solid particles with a polymer used as a component of potential hormone carrier as effective drug because of their ability to circulate and to release in a controlled period in the body. This review describes a variety of methods, characteristics and applications of nanoparticles hormones usages for animals. In general, several studies indicated that the formation of the hormone nanoparticles using polymer accompanied by distributing a good and stable of molecular mass, can be used as a carrier component of hormones as well as considering the negative effect.

The Potency and Quality of Goat’s Semen for Technological Application of Artificial Insemination Fitra Aji Pamungkas
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 19, No 1 (2009): MARCH 2009
Publisher : Indonesian Center for Animal Research and Development

The productivity of local goat is still relatively lower compared to that of other breed for sub-tropic area. Efforts for increasing its productivity through crossbreeding with genotypes goat could be approached by technological application of artificial insemination (AI). In supporting this technology, the viability of semen for both quality and quantity is needed. Evaluation of Indonesian goat semen characterisation shows a potency for frozen semen. The survive ability of sperm in fresh semen is very limited therefore reducing the temperature to -5°C (chilled semen) or -196°C (frozen semen) could be done to maintain its survive ability. Optimalization of frozen semen could be done by diluting in Tris extender with 6% glycerol, equilibrating for 4 hours and cooling for 4 – 5 minutes above surface of LN2 before stored in LN2 (-196°C). Thawing at > 7°C for 30 seconds resulted in the highest percentage of mortility (52.0%) and survivability (65.03%). Chilled semen is the best alternative for artificial insemination (AI) in the field condition where the supply of container and liquid nitrogen are limited. The survivability of chilled semen could be maintained for 8 days and the highest percentage of pregnancy resulted from chilled semen stored up to 24 – 48 hours. Key words : Goat, semen quality, frozen semen, chilled semen, artificial insemination

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