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All Journal WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences
Susan Maphilindawati Noor
Indonesian Research Center for Veterinary Science (IRCVS)
Veterinary

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Awareness of Emerging Glanders in Horses in Indonesia Susan Maphilindawati Noor; T Ariyanti
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 29, No 3 (2019): September 2019
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.322 KB) | DOI: 10.14334/wartazoa.v29i3.2061

Abstract

Glanders is a zoonotic disease that is highly contagious in animals and humans, caused by Burkholderia mallei. The clinical manifestations of glanders in horses are in the form of lesions on skin, nose and lungs. Horses play a role in transmitting glanders to healthy animal populations around them due to latent infections. Infection of glanders in humans is acute causing respiratory failure and could be fatal without proper treatment. Recently re-emerging glanders is reported in several countries that have eradicated diseases such as in India, Germany and China. The status of glanders in Indonesia is declared free, but surveillance showed positive antibodies to B. mallei in horses, as had been reported in 1939 and in 2018 in Jakarta. Glanders has a negative impact on a country's economy resulting in restrictions on international trade. Prevention of emerging glanders to Indonesia needs to be alerted because there is no effective treatment, no vaccines available for prevention and causing negative economic impacts. This paper aims to discuss glanders in horses, countermeasures through monitoring and surveillance, early detection in order to increase awareness of emerging glanders in Indonesia.
DNA Amplification Technique for Detection of Bovine Brucellosis Susan Maphilindawati Noor
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 28, No 2 (2018): June 2018
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.58 KB) | DOI: 10.14334/wartazoa.v28i2.1829

Abstract

Brucellosis is one of cattle diseases which causes a very significant economic loss and categorized as zoonotic disease. Early detection of Brucellosis in livestock is very important to prevent the spread of disease to livestock and humans. The success of Brucellosis control depends on rapid, sensitive and specific detection methods. The aim of this paper is to review several methods of Brucellosis detection in cattle. Currently, the detection of Brucellosis in Indonesia is using serological and isolation methods. The latter method is the gold standard of Brucellosis diagnosis, however, its sensitivity is low. Therefore, molecular techniques with DNA amplification have been developed and applied in many countries both in livestock and humans because they are more sensitive, specific and rapid in detecting Brucella sp in blood, milk and semen samples. Various DNA amplification methods for detection of Brucellosis that have been developed including polymerase chain reaction (PCR), finger printing and loop-mediated isothermal amplificatiom (LAMP). Both PCR and LAMP are more sensitive and specific in detecting Brucella sp than conventional techniques. PCR technique has advantages in detecting Brucella sp species to serotype and biovar levels. In addition, PCR reagents are cheaper and easier to obtain than LAMP eventhough, LAMP procedure is simpler and faster.
Co-Authors T Ariyanti