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All Journal Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences
S Sopiyana
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Veterinary

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Development and Conservation of Gonadal Primordial Germ Cells for Preservation of Local Chicken in Indonesia Tatan Kostaman; S Sopiyana
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 26, No 3 (2016): SEPTEMBER 2016
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.097 KB) | DOI: 10.14334/wartazoa.v26i3.1394

Abstract

One of the ex situ conservation techniques for poultry that recently developed was to collect primordial germ cell (PGC) or gonadal primordial germ cell (gPGC) that isolated from embryo development. Primordial germ cells (PGC) are embryonic cells that migrate to the gonads and form the precursors of gametes. The unique nature and accessibility of PGC during the early development provides an opportunity to manipulate the poultry germplasm, for example by forming germline chimeras. There are some stages that must be done through isolation and collection of PGC from its resources i.e. blastoderm, embryonic circulation blood and gonad. PGC collection originating from the gonads is one of existing PGC resources and technologies. gonadal PGC have advantages compared with other sources, namely (1) A large number of gonadal PGC can be taken from an embryo; and (2) A collection of gonadal PGC can be used in developing management systems of local avian germplasm conservation. This review is intended to describe the usefulness of isolation and collection technology of gonadal PGC as the local poultry germplasm conservation in Indonesia.
Analysis of Meat Mineral Content in Cemani Chicken with Homozygous (Fm/Fm) and Heterozygous (Fm/fm+) Genotypes Rona Saumy Safitry; A B Dharmayanthi; K Kinoshita; T Akiyama; S Darwati; Tatan Kostaman; S Sopiyana; I Khaerunnisa; Cece Sumantri
Jurnal Ilmu Ternak dan Veteriner Vol 27, No 4 (2022): December 2022
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v27i4.3075

Abstract

Cemani chicken is an Indonesian native chicken with black hyperpigmentation on feathers, skin, beak, comb, and flesh. Hyperpigmentation in chickens is called Fibromelanosis. Fibromelanosis in Cemani chickens is semi–dominant, producing two genotypes: homozygous (Fm/Fm) and heterozygous (Fm/fm+). Cemani chicken meat’s black color may indicate a higher mineral content than regular chicken meat. The study’s is aims are to detect genotype homozygous (Fm/Fm) and heterozygous (Fm/fm+) mutations and to determine the mineral content of Cemani chickens with homozygous (Fm/Fm) and heterozygous (Fm/fm+) genotypes. In the Cemani chicken population (n = 32), the Fm–specific allele genotype was detected using a Polymerase Chain Reaction–Restriction Fragment Length Polymorphism (PCR–RFLP) and the MluI restriction enzyme. The mineral contents of Cemani chicken tested were Fe, Zn, Mn, and Se. The results showed that homozygous Cemani chickens (Fm/Fm) had higher Fe and Zn mineral content. However, the homozygous (Fm/Fm) and heterozygous (Fm/fm+) Cemani chicken were not different in mineral content of Se and Mn. This study found that different genotypes of Cemani chicken had different mineral compositions. In the future, this analysis supports the selection of chicken strains with high antioxidant levels.
Co-Authors A B Dharmayanthi Cece Sumantri I Khaerunnisa K Kinoshita Rona Saumy Safitry S Darwati T Akiyama Tatan Kostaman