Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal JURNAL TEKNOLOGI LINGKUNGAN BERITA BIOLOGI
Djadja Siti Hazar Hoesen
Puslitbang Biologi, LIPI, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

Published : 10 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : JURNAL TEKNOLOGI LINGKUNGAN

 1 
TEKNIK BUDIDAYA IN VITRO Eleutherine sp. (Bawang Sabrang) Hoesen, Djadja Siti Hazar
Jurnal Teknologi Lingkungan Vol. 11 No. 3 (2010)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1146.938 KB) | DOI: 10.29122/jtl.v11i3.1179

Abstract

Vegetative propagation from bulb excised of Eleutherine sp. (Iridaceae) were cultured in Murashige and Skoog (MS) medium supplemented with plant growth regulator (PGR) Benzyl adenine (BA) 1 mg/l at initiation stage, BA (2 and 5) mg/l for induced shoot buds formation at multiplication stage. In this study also BA 2 mg/l and BA 2 mg/l combined with naphthalein acetic acid (NAA) 0.5 mg/l were treated for rooting planlets formation. Calli formation were induced with auxin PGR picloram 1 mg/l combined with 2,4 dichlorophenoxy acetic acid (2,4-D) 0.5 and 1 mg/l in concentrations. The media contained 2 mg/l cytokinin (BA) without auxin (NAA), resulted the highest shoot buds formation. Rooting planlets were produced in MS medium combined with BA and NAA.MS medium contains Picloram 1 mg/l and 2,4-D 1 mg/l was optimal for frequency oncalli initiation (100%) and the largest diameter of calli also represented in cultured MS medium with picloram 1 mg/l and 2,4-D 1mg/l. In acclimatization stage, 100% of planlets survived and successfully transplanted to soil medium in the field.Key words: in vitro, Eleutherine sp.
PEMBENTUKAN TUNAS Lilium sp. SECARA EX VITRO DAN IN VITRO Hoesen, Djadja Siti Hazar
Jurnal Teknologi Lingkungan Vol. 10 No. 2 (2009)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.926 KB) | DOI: 10.29122/jtl.v10i2.1491

Abstract

Buds, planlets and bulblets formation from excised bulbscales was the preferredmethod for vegetative propagation of Lilium sp (Liliaceae). The ex vitro techniqueswith Gibberellic acid (GA3) pretreatment was induced buds formation on scalescutting which planted on sterilized sand media. Buds rised from basal scales 7days after planted. However scales untreated GA3 obtained in 35-42 after planted.In vitro methods to promote buds initiated from bulbscales explants, was inducedon media MS (Murashige and Skoog) supplemented with GA3 1 mg/l. Media forinduced buds formation, MS contained Benzyl adenine (BA) 1 mg/l and 2 mg/lincreased multiple shoots formation significantly compared cultured on mediawithout BA. Roots growth improved on media contained NAA, but the highestplanlets achieved on cultured MS media without BA. Bulblets formation obtainedon media contained higher concentration of BA (5 mg/l).
Co-Authors Danu Danu, Danu Ning Wikan Utami Sukamto, LA Witjaksono Witjaksono