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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Sumberdaya Hayati BERITA BIOLOGI BIOMA
I MADE SAMUDRA
Indonesian Centre for Agricultural Biotechnology and Genetic Resources Research and Development (ICABGRD)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology

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Insecticidal Activity of Extracellular Protein of PRU8 Isolate against Tenebrio molitor Larvae Alina Akhdiya; Etty Pratiwi; I Made Samudra
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 2 (2009): June 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i2.2684

Abstract

Sel ataupun supernatan bebas sel dari kultur cair isolat bakteri entomopatogen PRU8 memiliki toksisitas tinggi terhadap larva Tenebrio molitor. Bioesei protein ekstrasel kasar hasil pengendapan amonium sulfat menunjukkan bahwa toksisitas tertinggi terdapat pada protein yang diendapkan pada kejenuhan amonium sulfat 70%. Pemurnian protein menggunakan kolom Hi Prep 16/60 Sephacryl S-200 HR menghasilkan satu fraksi protein toksin. Mortalitas larva uji yang disuntik dengan 19,2 nanogram toksin murni mencapai 71%. Uji toksisitas menggunakan toksin murni menunjukkan bahwa toksin tersebut termasuk kelompok toksin tipe injeksi. Berdasarkan analisa SDS-PAGE toksin tersebut tersusun atas dua protein dengan berat molekul 116,25 dan 66,24 kDa
Biologi Penggerek Batang Jagung Ostrinia furnacalis Gueneé yang diberi Pakan Buatan ARIF MUKHTAR HASBI; RIKA RAFFIUDIN; I MADE SAMUDRA
Jurnal Sumberdaya Hayati Vol. 2 No. 1 (2016)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.2.1.13-18

Abstract

Ostrinia furnacalis is a corn stem-borer that develops complete metamorphosis and all stages in life cycle in corn. This research was aimed to examine several biology aspects of O. furnacalis such as life cycle, egg incubaton period, egg fertility, female fecundity, longevity of imago, and copulation time on artificial diet, based on the previous study. The results of the observations showed that the life cycle of artificial-diet-given O. furnacalis was between 27-34 days range. Female fecundity was 16-452 eggs with fertility rate of 61,97% and 3-5 days renge of egg incubation period. Longevity of imago was between 6-11 days range, and the longevity was longer in female compared to the male. The imago of O. furnacalis copulate on 0-3 days after emerge from pupae and the highest number in on the day 1. Copulation time was occurred at 3-8 hour after scotophase commenced and the highest was at third hour. The artificial diet used in this research can be used for O. furnacalis mass rearing purpose and performed shorter length of egg stage until pupal stage compared to mass rearing with natural diet.
KARAKTERISASI BAKTERI PENGHASIL ASAM INDOL ASETAT DAN PENGARUHNYA TERHADAP VIGOR BENIH PADI Puji Lestari; Yadi Suryadi; Dwi Ningsih Susilowati; Tri Puji Priyatno; I Made Samudra
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1859

Abstract

The ability to produce indole acetic acid (IAA) by endophytic bacteria is one of the basic criteria for the use of bacteria as plant growth promoter agent which is essential for the agricultural production.The objectives of this study were to evaluate the ability of 17 bacterial isolates to produce IAA and its effect on improvement of rice seed germination and molecular identification of the selected isolates based on the 16S rRNA gene. The IAA content was determined using Salkowski method measured by spectrophotometer UV-Vis and the effect of endophytic bacteria inoculation on seed germination was done by in vitro assay. Sequences of the selected isolates 16S rRNA amplified by PCR were analyzed the homology against bacterial 16S rRNA database in Genebank. IAA values ranged from 6.632 to 50.053 mg/L with the highest IAA production shown by isolate 6KJ which was followed by 4PB (41.807 mg/L). Bacterial IAA increased rice seed vigor significantly compared to control. However, bacterial inoculation with different concentrations of IAA did not significantly affect the growth of rice plants. Based on the IAA and its effect on seed vigor, 6KJ, 4PB and 2KB were selected for molecular identification. Results showed that the three isolates belonged to Bacillus genus, 6KJ as B. aryabhattai, 4PB belonging to B. cibi and 2KB having 97% homology with B. marisflavi. Further evaluation of the selected endophytic isolates producing IAA is necessary to be carried out to explore their potency as a source of hormone to promote plant growth.
KARAKTERISASI KITINASE ISOLAT BAKTERI RHIZOSFIR ASAL CIANJUR DAN AKTIVITASNYA TERHADAP PATOGEN Colletotrichum sp. Yadi Suryadi; Dwiningsih Susilowati; I Made Samudra; Mustika Permatasari; Laksmi Ambarsari
Bioma : Jurnal Ilmiah Biologi Vol. 9 No. 1: April 2020
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v9i1.6034

Abstract

Kitinase mampu menghidrolisis kitin dan berpotensi sebagai agen biokontrol. Tujuan penelitian adalah memurnikan dan mengkarakterisasi kitinase dari isolat terpilih, serta menentukan kemampuan kitinolitiknya dalam penghambatan jamur Colletotrichum sp. Aktivitas kitinase ditentukan dengan metode Spindler, sementara kadar protein ditentukan dengan metode Bradford. Produksi kitinase isolat rizobakteri (isolat C5C) menunjukkan aktivitas enzim spesifik tertinggi sebesar 0.0489 U/mg melalui pemurnian parsial NH4SO4 70%, serta dapat meningkatkan kemurnian 13.97 kali dibandingkan ekstrak kasar. Karakterisasi kitinase isolat C5C menunjukkan bahwa enzim aktif optimal  pada suhu 55°C, pH 7, waktu inkubasi 120 menit, serta memiliki nilai Km sebesar 1.300 x 103 mg/L dan Vmaks sebesar 0.0294 mgL-1detik-1. Aktivitas antifungi pada uji in vitro menunjukkan bahwa isolat C5C dapat menghambat pertumbuhan Colletotrichum sp.Kata kunci: biokontrol,Colletotrichum sp.,  kitinase, rizobakteri
Co-Authors ALINA AKHDIYA ARIF MUKHTAR HASBI DWI NINGSIH SUSILOWATI Dwiningsih Susilowati Etty Pratiwi LAKSMI AMBARSARI Mustika Permatasari Puji Lestari RIKA RAFFIUDIN Tri Puji Priyatno Yadi Suryadi