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Unggul P. Juswono
Jurusan Fisika Fakultas MIPA Universitas Brawijaya Malang
Environmental Science

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Effect of Rosella Petal Extract (Hibiscus sabdariffa L.) On SOD Activity and Mouse Eye Rat (Rattus Wistar) Exposed to Gamma Radiation Rara Pramita; Chomsin S. Widodo; Unggul P. Juswono
Natural B, Journal of Health and Environmental Sciences Vol 2, No 4 (2014)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.225 KB) | DOI: 10.21776/ub.natural-b.2014.002.04.13

Abstract

Measurement activity of superoxide dismutase (SOD) and Malondhyaldehyde (MDA) to analyze the effect of roselle calyx extract (Hibiscus sabdariffa L) after exposure 300 rad gamma radiation. 35 male white rats (Rattus Wistar) were exposed to fractination dose of 300 rad for 7 day, are given in the sonde 2cc/days roselle extract for 16 days with a dose variation 62.5 mg/200 g BW, 125 mg/200 g BW, 250 mg/200 g BW, 400 mg/200 g BW, and 550 mg/200 g BW. Measurement of the activity of SOD and MDA were done by thiobarbituric acid (TBA) methode. Extract Roselle (Hibiscus sabdariffa L) 250 mg/200 gr BW increase the activity of superoxide dismutase (SOD) and decrease Malondialdehyde (MDA) after being exposed to radiation of 300 rad.
Studies on the Effect of Pollutant Waste on Free Radical Content in Tilapia Gill Organs (Oreochromis Niloticus) Nindha A. Berlianti; Chomsin S. Widodo; Unggul P. Juswono
Natural B, Journal of Health and Environmental Sciences Vol 2, No 4 (2014)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (589.771 KB) | DOI: 10.21776/ub.natural-b.2014.002.04.10

Abstract

Studies on the effect of heavy metal pollution on the appearance of free radicals in aquatic biota has been done by applying heavy metals Pb, Cd and Hg in tilapia fish (Oreochromis Niloticus). Free radicals research was done to organ of tilapia gills using ESR. The result showed that the concentration of Pb Nitrate 0.2 ppm – 1.4 ppm, Cd Nitrate 0.02 ppm – 0.14 ppm and Hg Nitrate 0.006 ppm – 0.018 ppm could improve free radical O2-. Free radicals concentration in organ of tilapia gills will increase with increasing concentration of heavy metals.
Effects of Gamma Radiation Exposure and Giving of Green Bean Sprout Extract (Phaseoulus radiatus) to Gonad Animal Fertility Rate of Mice Feris Kamlasi; Unggul P. Juswono
Natural B, Journal of Health and Environmental Sciences Vol 2, No 4 (2014)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (300.549 KB) | DOI: 10.21776/ub.natural-b.2014.002.04.14

Abstract

Gamma rays is an ionizing radiation that can causes infertilities.Cell defense mechanism against free radicals are involving active compound called antioxidant. Goal of this research is aim to analyze the influence of gamma radiation and review the influence of green beansprout extract (Phaseoulus radiatus) in gonad fertility rate with threevariables there are motility, viability and abnormality of spermatozoa. Male mice divided into three groups, control (K), non-extract radiation (RNE), and radiation plus extract (R+E).Mice was treated orally by green bean sprout extract with several dose 0.2 mL kg-1 body weight, 0.4 mL kg-1 body weight, 0.6 mL kg-1 body weight, 0.8 mL kg-1 body weight, and 1.0 mL kg-1 body weight. Radiation was given by fractionation starting from 100 rad, 150 rad, 200 rad, 250 rad, and 300 rad.The result shows that increasing radiation dose can reducing gonad fertility rate and treatment of Phaseoulus radiatus extract can suppress this reduction and preserve gonad fertility rate. 
The Effect of Exposure to Gamma Radiation And Giving Extracts Of White Watermelon (Citrullus vulgaris Schrad) Part of Kidney Health In Animal Trying Mice Pujianti B. Donuata; Muhaimin Rifa'i; Unggul P. Juswono
Natural B, Journal of Health and Environmental Sciences Vol 2, No 4 (2014)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (823.346 KB) | DOI: 10.21776/ub.natural-b.2014.002.04.15

Abstract

Cancer is a worldwide problem. People take medication in late stadium therefore radioteraphy plays important role in healing cancer. Side effect of cancer therapy such as cancers of urethra, colon, stomach or rectum may have bad influence for kidney which causes the decrease kidney function. Goal of this research is to understand the influence of gamma radiation on urine and kidney tubules necrosis. Mice assigned into two groups, which are extract and non-extract radiation groups. Each group has five variations of radiation doses, including 200rad, 250rad, 300rad, 350rad, and 400rad. White part extract of watermelon (Citrullus vulgaris Schrad) also used with five variations including 0.3mL, 0.6mL, 0.9mL and 1.2mL. Non-extract radiation group was only exposed to radiation without treatment with white part extract of watermelon. Profile of kidney health can be seen from the percentage of epithelium, erythrocyte and leukocyte after mice were put into metabolism cage for 24hours and percentage of kidney tubules necrosis observed under Microscope BX-51 and analyzed with Ms.Excel. Result of research concludes that gamma radiation increase epithelium, erythrocyte and leukocyte in urine, and kidney tubules necrosis. White part extract of watermelon can improve kidney health by reducing the percentage of epithelium, erythrocyte leukocyte and kidney tubules necrosis of mouse.
Co-Authors Chomsin Sulistiya Widodo Donuata, Pujianti Bejahida Feris Kamlasi Muhaimin Rifa'i Nindha A. Berlianti Rara Pramita