Article Per Year (5 Year)
p-Index From 2020 - 2025
0.444
P-Index
This Author published in this journals
All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Natural B
Nunung Harijati
Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Calcium Oxalate Crystals (CaOx) on Porang (Amorphopallus muelleri Blume) Exposed and Unexposed Sun Nurul Chairiyah; Nunung Harijati; Retno Mastuti
Natural B, Journal of Health and Environmental Sciences Vol 1, No 2 (2011)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (66.045 KB) | DOI: 10.21776/ub.natural-b.2011.001.02.6

Abstract

Sunlight was suspected to affect CaOx crystal formation in porang. This research had aim to determine variations in forms of CaOx crystals and the influence of shade on the density of CaOx crystals in porang. Preparations for microscopic observation derived from sliced leaf, petiole, and tuber of porang that grown under shaded and exposed to sunlight conditions. Sliced organ was cleared by using the modified clearing method. The parameters that observed included shape, shape variety, and density of CaOx crystals. CaOx crystal density were analyzed using ANOVA followed by Tukey (α 0.05). The differences of crystal density between the edges and middle of the organ were analyzed using Paired Samples T Test. Microscopic observation showed that CaOx crystals were grouped into large (20-710 m) and small (1-15 m) crystals size. The density of CaOx crystals in plants exposed to sunlight was 3 times higher than the shaded plants. Leaf organ had the highest number of crystal compared to others organ. The tuber had the lowest density of CaOx crystals among organ. In addition, the shaded or exposed to sunlight condition had no effect on CaOx crystal density between the edges and center of the organ.
The Dynamic of Calcium Oxalate (CaOx) in Porang Corms (Amorphophallus muelleri Blume) at Different Harvest Time Nurul Chairiyah; Nunung Harijati; Retno Mastuti
Journal of Tropical Life Science Vol. 11 No. 1 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.01.05

Abstract

The research aims to observe the influence of harvesting time on the change of calcium oxalate (CaOx) content and crystal density in Porang corms. The corms were harvested at different times, i.e., (1) two weeks before the plants shed (R0-1), (2) when the plants shed (R0), and (3) two weeks after the plants shed (R0+1). CaOx was obtained using the modified extracting method. Microscopic observations were obtained from the slices of the edge and center part of porang corms. Parameter observed including CaOx content, corm weight, shape, and density of CaOx crystal. CaOx content and crystal density in corms were analyzed using One way ANOVA. If the results are significant, it will be followed by Tukey Test α 0.05. In the meantime, the relation between CaOx content and corm weight was analyzed using Correlation Test Bivariate. The results showed that CaOx content was relatively higher in porang corms, i.e., 15.98 ± 0.60g/100g. On the other hand, the increasing of CaOx content might improve corm weight. The total density of druse, styloid, and prism crystal was pretty high in corms obtained when the plants shed compared to another harvest time, i.e., 1,494 ± 286; 31,280 ± 17,406 and 6,256 ± 1,533 crystals/cm2 . Raphide crystal density, by contrast, increased in corms obtained after the plants shed, i.e.,1,656 ± 368 crystals/cm2 . Total CaOx crystal density in the edge parts of corms harvested when the plants shed was proportionately higher than in the other harvest times, i.e., 12,292 ± 4,687.89 crystals/cm2 . In contrast, CaOx crystal densities in the center parts of corms were not much different at three harvesting times. The density of druse and prism crystals was somewhat higher in the center part of corms than in the edge parts. In opposition to, the density of raphide and styloid crystals was fairly higher in the edge part of corms than it was in the center parts. However, only raphide crystal density found in the edge and center part of corms was significantly affected by harvest time from all these results.
Physiological and Genetic Variations of Amorphophallus variabilis in Bojonegoro based on Glucomannan Content, Calcium Oxalate and RAPD Markers Achmad Yogi Pambudi; Nunung Harijati; Estri Laras Arumingtyas
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1169.253 KB) | DOI: 10.21776/ub.jels.2019.010.01.09

Abstract

This study had purpose to determine the glucomannan and calcium oxalate content in 7 variants of A. variabilis and their genetic relationships based on RAPD markers. Amorphophallus variabilis Tuber samples were taken from Karangdowo village, Sumberrejo Sub-district, Bojonegoro District, East Java. Each variant was analyzed for its glucomannan content by the spectrophotometric method using 3,5-DNS reagents and calcium oxalate by the 0.1N KMnO4 titration method. Leaf DNA extraction was carried out using the CTAB method. Relationship analysis used RAPD markers with 5 primers (OPA-11, OPC-04, OPU-06, OPC-07, and OPN-1). The obtained data were analyzed using the Numerical Taxonomy and Multivariate System (NTSYS-pc) version 2.1. The grouping of glucomannan content or oxalium oxalate used hierarchical clustering analysis (SPSS 16.0). This research found that the calcium oxalate content in seven variants of A. variabilis ranged from 0.01 to 0.03 g, where the variant with the lowest calcium oxalate is V6 with a value of 0.01 g and the highest is the V7 variant with a value of 0.03 g, while the glucomannan content ranges from 9 - 38%. The highest glucomannan content is V3, while the lowest is V6. Phenograms formed based on RAPD markers showed the formation of two groups of A. variabilis. Group one has two subgroups. Subgroup one consists of variants V1 and V4, while subgroup two consists of V6 and V7. Meanwhile, the second group consists of variants V2, V3, and V5. The seven variant grouping pattern of A. variabilis based on RAPD markers has no similarity to the grouping pattern based on the results of glucomannan or calcium oxalate analysis.
Co-Authors Achmad Yogi Pambudi Estri Laras Arumingtyas Nurul Chairiyah Nurul Chairiyah Retno Mastuti Retno Mastuti