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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology International Journal of Advances in Intelligent Informatics
Tri Handayani
Indonesian Institute of Science
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Immunology & microbiology Veterinary

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Mathematics and statistics related studies in Indonesia using co-authorship network analysis Irene Muflikh Nadhiroh; Ria Hardiyati; Mia Amelia; Tri Handayani
International Journal of Advances in Intelligent Informatics Vol 4, No 2 (2018): July 2018
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26555/ijain.v4i2.120

Abstract

Indonesian scholars have published a numbers of articles in numerous international publications, however, it still lags behind other Singapore, Malaysia, and Vietnam. This article performs a bibliometrics analysis and examine the collaboration network in Mathematics and Statistics related subject of scholars with Indonesian affiliation as recorded in Web of Science. In total, based on article publications during 2009-2017, 426 articles were retrieved. Bandung Institute of Technology (ITB) was the affiliation with the highest number of articles (48%) and number of authors (27%). Using Social Network Analysis to examine co-authorship networks, this research shows that the co-author network has the highest centrality in the ITB affiliation. Meanwhile, dependency of foreign affiliation is still high, shown as a high percentage (84% of all articles) of international co-authorship. Co-authorship network of Mathematics and Statistics related studies in Indonesia possesses as a scale-free network and followed the power law distribution. This research showed the achievement of Indonesian scholars of Mathematics and Statistics, and can be used to evaluate the knowledge transfer in these subjects and related areas.
BINARY VECTOR CONSTRUCTION AND Agrobacterium tumefaciens-MEDIATED TRANSFORMATION OF LYSOZYME GENE IN SEAWEED Kappaphycus alvarezii Tri handayani; Alimuddin Alimuddin; Utut Widyastuti; Emma Suryati; Andi Parenrengi
BIOTROPIA Vol. 21 No. 2 (2014): BIOTROPIA Vol. 21 No. 2 December 2014
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2014.21.2.348

Abstract

Ice-ice disease is the biggest problem in the cultivation of seaweed Kappaphycus alvarezii. The disease is caused by bacterial infection and induced by drastic changes of water quality. Lysozyme has the ability to break down bacterial cell wall. The purpose of this research was to construct of a binary vector pMSH1-Lys carrying chicken Lysozyme (Lis) gene and introduce pMSH1-Lis on K. alvarezii. The binary vector expression was transformed into Agrobacterium tumefaciens LBA4404 by triparental mating. Thallus was inoculated with A. tumefaciens carrying pMSH1-Lis and then the transformed thallus was selected by adding 20 mg/L hygromycin to the culture medium. PCR analysis showed that the construction of the binary plasmid pMSH1-Lys was established. Percentage of transformation of pMSH1-Lis on K. alvarezii was 23.56%, while the efficiency of regeneration was 11.32%. PCR analysis showed that three of the regenerated thallus contained Lysozyme gene. Thus, transgenic K. alvarezii was produced successfully and this can be useful for studying the mechanisms of seaweed defense against bacterial infection.
Co-Authors Alimuddin Alimuddin Andi Parenrengi Emma Suryati Irene Muflikh Nadhiroh Mia Amelia Ria Hardiyati UTUT WIDYASTUTI