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Identification of Water Bacteria from Nickel Post Mining in East Halmahera Margaretta Christita; Iwanuddin Iwanuddin; Yermias Kafiar; Supratman Tabba; Hendra S Mokodompit
Jurnal Wasian Vol 5, No 1 (2018): Jurnal Wasian
Publisher : Balai Penerapan Standar Instrumen Lingkungan Hidup dan Kehutanan (BPPLHK)Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jwas.v5i1.4265

Nickel mining impact in heavy metal pollution on both soil and water. A method that is widely applied to reduce heavy metal contamination is bioremediation. Selection of bacteria that have potential reduction of heavy metal contamination in soil is very important in bioremediation process. The first step to select the potential bacteria is identification of samples. The purpose of this study is to identify existings bacteria in pond after nickel mining area PT. Antam, East Halmahera. Methods used in this research are isolation and identification of bacteria by conventional methods includes morphological, physiologica,l and biochemical test. Identification using Bergey’s Manual Determinative. The results showed there are 6 genera types of bacteria there are Bacillus, Esherichia, Enterococcus, Pseudomonas, Staphylococcus, dan Klebsiella, with 18 species in water of nickel post mining. Morphologically it is dominated by genus Bacillus as 50 % of the species. The identification of bacteria proved an existence of indigenous bacteria which is resistant to heavy metal stress.Keywords: bacteria, soil, water, contamination, post mining, East Halmahera

Distribution of Avifauna in Aketajawe Lolobata National Park Based on Zone and Land Cover Typology Supratman Tabba; Lis Nurrani
Jurnal Wasian Vol 3, No 1 (2016): Jurnal Wasian
Publisher : Balai Penerapan Standar Instrumen Lingkungan Hidup dan Kehutanan (BPPLHK)Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jwas.v3i1.891

Aketajawe Lolobata National Park (TNAL) is a protected area for paradise birds, parrots and others North Maluku endemic wildlife. As a former of commercial logging concessions, this areas have been damaged and loss of wildlife habitat. The purpose of this research was to determine bird species of TNAL area based on zone category, this research is important in order to validate data related to the birds species diversity. Data collection used the observation point system with purposive sampling by direct observation and audio. The number of observation points were six points in core zone, ten points in both of jungle and traditional zone, and six points in buffer zone. Research showed that there are 39 species found in the buffer zone, 45 species in jungle and traditional zone, and 19 species in core zone. Wallace’s standardwing (Semioptera wallacei) is one of the 15 endemic species of North Maluku was found along drummer rail (Habroptila wallacii) which is Halmahera’s endemic species. Distribution pattern of birds in TNAL is very varied. The jungle and traditional zone have the amount and the highest species diversity while the highest species variation was in the buffer zone. The natural habitat of bird in this area generally located in open area along former road skid as well as forest edge and only a few were found in primary forest of core zone.

BIOAKTIVITAS Crotalaria striata DC DAN Cinnamomum cullilawan Bl TERHADAP SEL KANKER SERVIKS HeLa Lis Nurrani; Supratman Tabba; Arif Irawan
Buletin Penelitian Tanaman Rempah dan Obat Vol 27, No 1 (2016): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v27n1.2016.1-9

Kanker serviks merupakan jenis kanker mematikan yang secara spesifik menyerang kaum wanita. Berbagai cara pencegahan dilakukan terhadap penyakit ini melalui deteksi dini dengan menggunakan alat-alat kedokteran yang canggih, hingga penggunaan bahan-bahan alami yang berasal dari berbagai macam tumbuhan dengan cara-cara tradisional. Penelitian ini bertujuan untuk mengetahui bioaktivitas kulit kayu lawang (Cinnamomun cullilawan), dan daun kuhung-kuhung (Crotalaria striata) terhadap sel kanker serviks HeLa (ATCC CCl 2). Serbuk sampel dimaserasi dengan pelarut polar etanol kualitas teknis 70% dengan perbandingan 1:5 selama 24 jam. Konsentrasi ekstrak yang dimasukkan ke dalam cawan petri yang mengandung sel kanker adalah 25, 50, 100, 200 dan 500 µg ml-1. Hasil penelitian menunjukkan bahwa ekstrak daun C. striata memiliki aktivitas antikanker serviks lebih baik dibandingkan ekstrak kulit C. cullilawan. Ekstrak daun C. striata mampu membunuh sel kanker HeLa pada konsentrasi 635,289 µg ml-1, sedangkan ekstrak kulit kayu C. cullilawan baru mampu membunuh sel kanker HeLa dengan nilai IC50 sebesar 1.435,79 µg ml-1 (>1.000 µg ml-1). Kedua ekstrak tersebut tidak mengandung senyawa alfatokoferol dan quercetin.

Identification of Water Bacteria from Nickel Post Mining in East Halmahera Margaretta Christita; Iwanuddin Iwanuddin; Yermias Kafiar; Supratman Tabba; Hendra S Mokodompit
Jurnal Wasian Vol 5, No 1 (2018): Jurnal Wasian
Publisher : Balai Penerapan Standar Instrumen Lingkungan Hidup dan Kehutanan (BPPLHK)Manado

Nickel mining impact in heavy metal pollution on both soil and water. A method that is widely applied to reduce heavy metal contamination is bioremediation. Selection of bacteria that have potential reduction of heavy metal contamination in soil is very important in bioremediation process. The first step to select the potential bacteria is identification of samples. The purpose of this study is to identify existings bacteria in pond after nickel mining area PT. Antam, East Halmahera. Methods used in this research are isolation and identification of bacteria by conventional methods includes morphological, physiologica,l and biochemical test. Identification using Bergey’s Manual Determinative. The results showed there are 6 genera types of bacteria there are Bacillus, Esherichia, Enterococcus, Pseudomonas, Staphylococcus, dan Klebsiella, with 18 species in water of nickel post mining. Morphologically it is dominated by genus Bacillus as 50 % of the species. The identification of bacteria proved an existence of indigenous bacteria which is resistant to heavy metal stress.Keywords: bacteria, soil, water, contamination, post mining, East Halmahera

Distribution of Avifauna in Aketajawe Lolobata National Park Based on Zone and Land Cover Typology Supratman Tabba; Lis Nurrani
Jurnal Wasian Vol 3, No 1 (2016): Jurnal Wasian
Publisher : Balai Penerapan Standar Instrumen Lingkungan Hidup dan Kehutanan (BPPLHK)Manado

Aketajawe Lolobata National Park (TNAL) is a protected area for paradise birds, parrots and others North Maluku endemic wildlife. As a former of commercial logging concessions, this areas have been damaged and loss of wildlife habitat. The purpose of this research was to determine bird species of TNAL area based on zone category, this research is important in order to validate data related to the birds species diversity. Data collection used the observation point system with purposive sampling by direct observation and audio. The number of observation points were six points in core zone, ten points in both of jungle and traditional zone, and six points in buffer zone. Research showed that there are 39 species found in the buffer zone, 45 species in jungle and traditional zone, and 19 species in core zone. Wallace’s standardwing (Semioptera wallacei) is one of the 15 endemic species of North Maluku was found along drummer rail (Habroptila wallacii) which is Halmahera’s endemic species. Distribution pattern of birds in TNAL is very varied. The jungle and traditional zone have the amount and the highest species diversity while the highest species variation was in the buffer zone. The natural habitat of bird in this area generally located in open area along former road skid as well as forest edge and only a few were found in primary forest of core zone.

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