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All Journal Jurnal Ilmu Kelautan Spermonde Jurnal Biologi Tropis
Warsidah
Tanjungpura University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology

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OPTIMIZATION OF Î’-CAROTENE PRODUCTION IN Dunaliella salina USING LED AND DIFFERENT CULTURE MEDIA Mega Sari Juane Sofiana; Shifa Helena; Ikha; Warsidah
Jurnal Ilmu Kelautan SPERMONDE VOLUME 7 NOMOR 1, 2021
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jiks.v7i1.14767

Abstract

Dunaliella salina is a green microalgae that has the ability to produce β-carotene used in various fields, such as food supplements, natural colorant, antioxidants, anti-cancer, and anti-aging. D. salina is capable of producing large amounts of carotenoids under stressful conditions, including light and nutrient. Light is the main factor that stimulate the production of carotenoid pigments and media composition plays an important role for growth, biomass, and β-carotene production. The accumulation of β-carotene in microalgae is closely related to the type and quality of light and the composition of the culture media. This study aimed to determine the production of β-carotene in microalgae D. salina using LEDs and different culture media to select the best culture conditions for producing high value compounds. The results showed that D. salina cultured using red LED light and technical Walne media was able to produce β-carotene with the highest amount of 767,499 mg/100 g. In blue LED light and Walne Pro-analysis media, the β-carotene content was 380,522 mg/100 g, while the ZA+NPK media has the lowest value. In this study, Walne Pro-analysis media became the best culture medium for D. salina. Therefore, natural sources of β-carotene can be obtained from D. salina, so it could reduce the use of synthetic carotene in meeting global demand.
Macronutrient Content of Sipunculus nudus as an Antistunting Candidate Warsidah
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6244

Abstract

Consuming balanced nutritious food with macronutrient content including carbohydrates, protein and fat for pregnant and breastfeeding mothers as well as children under five during the growth and development period is one of the efforts to prevent and treat anti-stunting, up to the age of 2 years. Waters and beaches are one of the habitats for marine biota such as bivalve shells, gastropods, various fish and other marine biota whose names and uses are not yet popular but are widely consumed by coastal communities, including Sipunculus nudus. Marine biota from the phylum Sipuncula in some areas are called sea worms, are one of the marine biota whose habitat is in the sand, and are the favorite food of coastal communities. Information about the nutritional and mineral content of this marine biota species has not been widely reported. This research aims to determine the macronutrient content of Sipunculus nudus collected from the coastal waters of Samboang, South Sulawesi. Determination of macronutrients was carried out using several methods, including for protein using the Kjeldahl method, fat using extraction with n-hexane solvent, water and ash content using an oven and furnace. The results of measuring the macronutrient content of Sipunculus nudus showed a water content of 72%, ash content of 1.19%, protein content of 6.5%, fat content of 2.07% and carbohydrate content using difference calculations was obtained at 18.24%.
Co-Authors Helena, Shifa Ikha Mega Sari Juane Sofiana